Histone Variants as Stem Cell Biomarkers for Long‐Term Injection Medialization Laryngoplasty

Voss, Stephen G.; San‐Marina, Serban; Oldenburg, Michael S.; Ekbom, Dale C.; Madden, Benjamin J.; Charlesworth, M. Cristine; Janus, Jeffrey R.

doi:10.1002/lary.27429

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…Z that are associated with open chromatin states and ADSC proliferation were overrepresented in the MACD + ADSC group relative to structural histones. 13 In the present study, ADSCs co-injected with MX-JC did not boost medialization volumes relative to MX-JC alone. ADSCs, with well-described immunosuppressive effects, appeared to dampen the T cell-mediated immune response to MX-JC, characterized by dendritic cells and macrophage infiltration, which may explain this observation (Table I and Figure 6).…”

Section: Discussioncontrasting

confidence: 49%

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MRI Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty

et al. 2021

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Objectives/Hypothesis: Test a new jellyfish collagen biomaterial aimed to increase duration of injection medialization laryngoplasty (IL) against two products in clinical practice.Study Design: Animal model. Methods: Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits (N = 6/group). Group 1 received micronized cross-linked jellyfish collagen (MX-JC) and adipose derived stem cells (ADSCs), Group 2, MX-JC alone, Group 3, cross-linked hyaluronic acid (X-HA), and Group 4, micronized acellular dermis (MACD). Animals were sacrificed at 4 and 12 weeks. Major outcomes were MRI tissue volumes and histopathology.Results: After 100 μL IL MRI volumes (means AE STD) at 4 and 12 weeks were:

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Section: Discussioncontrasting

confidence: 49%

“…Fixed tissues were embedded in paraffin and 5 μm sections were cut and stained with hematoxylin and eosin as previously described. 9,13 Briefly, slides were deparaffinized and stained in Harris hematoxylin solution for10 minutes then rinsed 5 minutes in running tap water. After 1-5 sec.…”

Section: Histologymentioning

confidence: 99%

MRI Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty

et al. 2021

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“… 24 , 25 , 26 , 27 , 28 , 29 However, in a literature search on laryngeal proteomics following IL, the only entries were two previous studies from our group. 16 , 30 …”

Section: Discussionmentioning

confidence: 99%

“…The detailed protocol for nLC‐MS/MS analysis was previously reported. 16 Briefly, solubilization of collected tissue was performed in 2% sodium dodecyl sulfate (SDS)/50 mM Tris (pH 8.2), followed by reduction with 10 mM Tris‐(carboxyethyl) phosphine hydrochloride and cysteine alkylation with 10‐mM iodoacetemide. After SDS removal samples were digested with 1:50 trypsin solution and peptides were separated by nano‐liquid chromatography (nLC) using a Q‐Exactive mass spectrometer coupled to a Dionex 3000 Rapid Separation Liquid Chromatography nano liquid chromatograph (Thermo Fisher Scientific, Bremen, Germany), with a mixture of solvents containing water, formic acid, acetonitrile, and iso‐propanol at a rate of 400 nl/min using PicoFrit/Agilent Poroshell‐packed columns.…”

Section: Methodsmentioning

confidence: 99%

Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation

Bowen

Ekbom

Hunter

et al. 2022

Laryngoscope Investig Oto

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Objectives/Hypothesis Compare proteomic profiles of rabbit vocal folds (VFs) injected with micronized cross‐linked jellyfish collagen “collagen Type 0” (MX‐JC) against two clinical products for injection medialization laryngoplasty (IL). Study Design Animal model. Methods Left recurrent laryngeal nerve sectioning and IL were performed in New Zealand White rabbits ( N = 6/group). Group 1 received (MX‐JC) and adipose‐derived stem cells (ADSCs), Group 2, MX‐JC alone; Group 3, cross‐linked hyaluronic acid; and Group 4, micronized acellular dermis. Animals were sacrificed at 4 and 12 weeks. Proteomic profiling of injected versus noninjected VFs by nano‐liquid chromatography, tandem mass spectrometry, and reactome gene ontology analysis was performed. Results Overall, 37–61 proteins were found to be upregulated and 60–284 downregulated in injected versus non‐injected VFs (>1.5 fold, false discovery rate‐adjusted p < .05). Over‐representation analysis (% of total) revealed top up‐regulated pathways at 4 and 12 weeks, respectively: Group 1, keratan sulfate metabolism (46%) and cellular processes (29%); Group 2, extracellular matrix (ECM)/collagen processes (33%) and beta oxidation (39%); Group 3, cellular processes (50%) and energy metabolism (100%); and Group 4, keratan sulfate metabolism (31%) and inflammation (50%). Top downregulated pathways were: Group 1, Inflammation (36%) and glucose/citric acid metabolism (42%); Group 2, cell signaling (38%) and glucose/citric acid metabolism (35%); Group 3, keratan sulfate metabolism (31%) and ECM/collagen processes (48%); and Group 4, glucose/citric acid metabolism (33%) and ECM/collagen processes (43%). Conclusions MX‐JC “collagen Type 0” upregulates pathways related to ECM/collagen formation and downregulates pathways related to inflammation suggesting that it is promising biomaterial for IL. Level of Evidence NA

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“…This kind of specific marker for MSCs would be helpful for accurate evaluation of MSCs implantation approach for VF scars in the future [137]. Although, not for VF scar treatment, Voss et al recently demonstrated that histone variants are useful as ASCs biomarkers for long-term injection medialization laryngoplasty [138]. These markers would be helpful for the accurate identification of the stem cell differentiation process in the stem cell implantation approach for VF scar.…”

Section: Current Obstacles To Restoring Slp Pliabilitymentioning

confidence: 99%

Pathophysiology of Fibrosis in the Vocal Fold: Current Research, Future Treatment Strategies, and Obstacles to Restoring Vocal Fold Pliability

Kumai

2019

IJMS

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Communication by voice depends on symmetrical vibrations within the vocal folds (VFs) and is indispensable for various occupations. VF scarring is one of the main reasons for permanent dysphonia and results from injury to the unique layered structure of the VFs. The increased collagen and decreased hyaluronic acid within VF scars lead to a loss of pliability of the VFs and significantly decreases their capacity to vibrate. As there is currently no definitive treatment for VF scarring, regenerative medicine and tissue engineering have become increasingly important research areas within otolaryngology. Several recent reviews have described the problem of VF scarring and various possible solutions, including tissue engineered cells and tissues, biomaterial implants, stem cells, growth factors, anti-inflammatory cytokines antifibrotic agents. Despite considerable research progress, these technical advances have not been established as routine clinical procedures. This review focuses on emerging techniques for restoring VF pliability using various approaches. We discuss our studies on interactions among adipose-derived stem/stromal cells, antifibrotic agents, and VF fibroblasts using an in vitro model. We also identify some obstacles to advances in research.

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Histone Variants as Stem Cell Biomarkers for Long‐Term Injection Medialization Laryngoplasty

Abstract: NA.

Cited by 10 publications

References 31 publications

MRI Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty

MRI Study of Jellyfish Collagen, Hyaluronic Acid, and Cadaveric Dermis for Injection Laryngoplasty

Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation

Pathophysiology of Fibrosis in the Vocal Fold: Current Research, Future Treatment Strategies, and Obstacles to Restoring Vocal Fold Pliability

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