Histopathologic analysis of bone marrow and bone metastasis in murine neuroblastoma

Yoshino, K.; Tanabe, Masahiro; Ohnuma, Naomi; Takahashi, Hideyo

doi:10.1007/bf00128962

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Almost half of NBs have metastases at the time of diagnosis (3,17). Although the most common localization of metastases is the BM, metastases can be observed in the skeleton, lymph nodes, liver, and intracranial and orbital regions (3,4,6,7,17,21,22). Despite aggressive multimodality therapy, the long-term survival rate at diagnosis of metastatic disease for patients older than 18 months is still less than 30% (15).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Neuroblastomas (NB) are the most common extracranial solid tumors in children less than 15 years of age (1)(2)(3)(4)(5)(6)(7)(8)(9). They are responsible for approximately 15% of cancerrelated deaths in this age group (2)(3)(4)(5)(6)(7)(8)(9)(10). There are established parameters in the risk stratification of NB patients such as tumor differentiation and mitosis-karyorrhexis index (MKI), NMYC amplification, the patient's age, and tumor stage (11).…”

Section: Introductionmentioning

confidence: 99%

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The significance of histopathologic assessment in bone marrow disease in neuroblastoma

Ekmekçi¹,

İnce²,

Olgun³

et al. 2021

TJPATH

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Objective: Neuroblastoma (NB) is the most common extracranial solid tumor in children and is responsible for 12% of cancer-related deaths. The status of metastatic disease in the bone marrow (BM) is a predictor of poor outcome. The purpose of this study was to investigate the predictive significance of histopathological examination of BM in NB. Material and Method:The study included 61 cases with archival bone marrow biopsy tissues. The cases were evaluated regarding the percentage of metastatic tissue and its differentiation. Primary tumor slides were also reviewed to perform the Shimada classification based on the differentiation status and mitosis-karyorrhexis index. The patients' age, gender, NMYC amplification, clinical risk group, and disease outcome were also noted. Results:Of the 61 cases, 17 had BM involvement. Of those, eight cases (47.1%) were refractory NB showing disease relapse. Based on BM examination, five cases (29.4%) were categorized as complete response, seven (41.2%) as progressive disease, three (17.6%) as minimal disease, and two (11.8%) as stable disease. The progressive disease category was significantly related with refractory disease and NMYC amplification along with the high-risk category (p =0.002 and p= 0.003 respectively). Undifferentiated histology and presence of more than 20% of tumor tissue in the BM biopsy at diagnosis were significantly associated with the progressive disease category (p=0.01 and p<0.001, respectively). Conclusion:We conclude that evaluating the percentage of metastatic tumor tissue and tumor differentiation in BM biopsies is of clinical importance in the management of neuroblastoma patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The significance of histopathologic assessment in bone marrow disease in neuroblastoma

Ekmekçi¹,

İnce²,

Olgun³

et al. 2021

TJPATH

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show abstract

“…These low-throughput methods require invasive and potentially incomprehensive biopsies and usually suffer from labor-intensive tissue preparation procedures (5)(6)(7)(8)(9)(10). Fluorescence imaging (11) and bioluminescence imaging (12) (BLI) also have been applied to detect metastasized tumor cells labeled with optically active proteins.…”

Section: Introductionmentioning

confidence: 99%

Assessment of anti-metastatic drug efficacy via localization and quantification ofex vivo murine bone tumor load using high-throughput MRIT1 parametric analysis

Pagel¹,

Baldwin²,

Rader³

et al. 2006

NMR Biomed.

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MRI methods show great utility for assessing the growth of tumors metastasized to bone in clinical studies. However, preclinical MRI methods in rodents do not translate well to high-throughput studies of bone tumors, especially for early-stage tumors typically examined in pharmaceutical discovery efforts. To overcome these limitations, an ex vivo MR T1 parametric mapping method has been developed to measure metastasized bone tumor load in murine long bones. This method has been used to assess the therapeutic efficacy of SU11248, a multi-targeted inhibitor with demonstrated anti-tumor activity and reduction of bone loss, in a murine model of metastasized breast tumor cells. The results show precise localizations of relative tumor loads within the bones and reveal significant differences between SU11248-treated and untreated animal groups. The procedures were optimized for simultaneous, high-throughput parallel image acquisition of MRI data for 30 samples and included an automated segmentation method for image processing. The merits of this T1 parametric mapping method are compared with clinical T1-weighted MRI methods, histopathology and bioluminescence imaging of the same murine bone tumor model.

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Murine model for skeletal metastases of Ewing's sarcoma

Scotlandi

Benini

Manara

et al. 2000

Journal Orthopaedic Research

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Ewing's sarcoma shows a strong tendency to metastasize to the lungs or the skeleton, or both. A peculiar feature of the secondary involvement of bone with this tumor is that it may also appear in the absence of clinically evident lung metastases, both at clinical presentation and during the course of the disease. Although osseous metastases are critically relevant for prognosis, the pathogenesis of this peculiar feature of Ewing's sarcoma is poorly understood, partly due to the lack of appropriate experimental in vivo models. We show that the intravenous injection of TC-71 Ewing's sarcoma cells into athymic 4-5-week-old Crl/nu/nu (CD1) BR mice reproducibly colonizes specific sites of the skeleton in addition to the lungs and lymph nodes. The distribution and the morphologic appearance of these experimental bone metastases mimic the pattern of skeletal involvement observed in humans. This experimental model of bone metastasis of Ewing's sarcoma may be the basis for future studies aimed at understanding the pathophysiology and treatment of Ewing's sarcoma.

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Histopathologic analysis of bone marrow and bone metastasis in murine neuroblastoma

Cited by 5 publications

References 15 publications

The significance of histopathologic assessment in bone marrow disease in neuroblastoma

The significance of histopathologic assessment in bone marrow disease in neuroblastoma

Assessment of anti-metastatic drug efficacy via localization and quantification ofex vivo murine bone tumor load using high-throughput MRIT1 parametric analysis

Murine model for skeletal metastases of Ewing's sarcoma

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