Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila

Chen, Nan; Jiang, Jingjing; Gao, Xiaojian; Li, Xixi; Zhang, Yue; Liu, Xiaodan; Yang, Hui; Bing, Xuwen; Zhang, Xiaojun

doi:10.1016/j.fsi.2018.09.023

Cited by 60 publications

(25 citation statements)

References 33 publications

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“…infection. In liver, changes were manifested as necrosis of liver parenchyma [15,39,40]. Splenic necrosis was also observed, similarly reported by previous studies [39,40].…”

Section: Discussionsupporting

confidence: 77%

“…In liver, changes were manifested as necrosis of liver parenchyma [15,39,40]. Splenic necrosis was also observed, similarly reported by previous studies [39,40]. Necrosis of kidney was characterized by swollen, desquamated and vacuolated tubular epithelial cells with some cells having complete necrosis, while atrophied glomerulus were also reported in previous studies [15,40,41].…”

Section: Discussionsupporting

confidence: 75%

“…Necrosis of kidney was characterized by swollen, desquamated and vacuolated tubular epithelial cells with some cells having complete necrosis, while atrophied glomerulus were also reported in previous studies [15,40,41]. Other histopathological changes such as the presence of polymorphonuclear cells in the inter tubular area, along with proliferated cell of glomerulus, were not observed in this study [15,39]. Alternatively, the presence of haemocyte aggregation and congestion in all selected internal organs indicated that liver, spleen and kidney are the target organ for Aeromonas systemic infection [16,39,40].…”

Section: Discussionsupporting

confidence: 61%

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Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Azzam-Sayuti

Ina‐Salwany

Zamri‐Saad

et al. 2021

Biology

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The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of Aeromonas spp. were initially screened for the ability to grow on M9 agar with myo-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent A. hydrophila (vAh) pathotype. Subsequently, LD50 of six selected Aeromonas spp. were determined by intraperitoneal injection of bacterial suspension containing 103, 105, and 107 CFU/mL of the respective Aeromonas sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only A. dhakensis 1P11S3 was able to grow using myo-inositol as a sole carbon source, and no vAh strains were identified. The LD50–240h of A. dhakensis 1P11S3 was 107 CFU/mL, while the non-myo-inositol utilizing A. dhakensis 4PS2 and A. hydrophila 8TK3 was lower at 105 CFU/mL. Similarly, tilapia challenged with the myo-inositol A. dhakensis 1P11S3 showed significantly (p < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-myo-inositol A. dhakensis 4PS2 and A. hydrophila 8TK3. These findings suggested that myo-inositol utilizing A. dhakensis 1P11S3 was not a hypervirulent Aeromonas sp. under current experimental disease challenge conditions, and that diverse Aeromonas spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of myo-inositol utilizing ability on the pathogenesis of Aeromonas spp., since this ability correlates with hypervirulence in A. hydrophila strains.

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“…infection. In liver, changes were manifested as necrosis of liver parenchyma [15,39,40]. Splenic necrosis was also observed, similarly reported by previous studies [39,40].…”

Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 61%

See 1 more Smart Citation

Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Azzam-Sayuti

Ina‐Salwany

Zamri‐Saad

et al. 2021

Biology

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“…Among of which, bacterial infections have become the main obstruction to freshwater farming [33]. Epidemic disease caused by A. hydrophila has been reported, which may result in millions of dollars' economic losses worldwide [34].…”

Section: Discussionmentioning

confidence: 99%

Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin

et al. 2020

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“…Histologically, liver cell of hypothermic rats appear normal, unlike the ones of the normothermic ones, suggesting that the radiation dosage was high enough to induce damage visible after a short time (4 h). Liver cells are extensively vacuolised, a sign that was reported to be a response to a stress agent [30,31]. Testicles also appear to be damaged by the irradiation in the normothermic group.…”

Section: Discussionmentioning

confidence: 99%

Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor

Tinganelli

Hitrec

Romani

et al. 2019

IJMS

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Hibernation has been proposed as a tool for human space travel. In recent years, a procedure to induce a metabolic state known as “synthetic torpor” in non-hibernating mammals was successfully developed. Synthetic torpor may not only be an efficient method to spare resources and reduce psychological problems in long-term exploratory-class missions, but may also represent a countermeasure against cosmic rays. Here we show the preliminary results from an experiment in rats exposed to ionizing radiation in normothermic conditions or synthetic torpor. Animals were irradiated with 3 Gy X-rays and organs were collected 4 h after exposure. Histological analysis of liver and testicle showed a reduced toxicity in animals irradiated in torpor compared to controls irradiated at normal temperature and metabolic activity. The expression of ataxia telangiectasia mutated (ATM) in the liver was significantly downregulated in the group of animal in synthetic torpor. In the testicle, more genes involved in the DNA damage signaling were downregulated during synthetic torpor. These data show for the first time that synthetic torpor is a radioprotector in non-hibernators, similarly to natural torpor in hibernating animals. Synthetic torpor can be an effective strategy to protect humans during long term space exploration of the solar system.

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Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila

Cited by 60 publications

References 33 publications

Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin

Hibernation and Radioprotection: Gene Expression in the Liver and Testicle of Rats Irradiated under Synthetic Torpor

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