Histopathological and Digital Morphometrical Evaluation of Uterine Leiomyoma in Brazilian Women

Silva, Ana Paula Fernandes da; Mello, Luciano de Albuquerque; Santos, Erlene Roberta Ribeiro dos; Paz, Silvânia Tavares; Cavalcanti, C. L. B.; Melo-Júnior, Mário Ribeiro de

doi:10.1155/2016/2968410

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…1 These are the most common benign tumors of uterus affecting 20-40% women of reproductive age group. [2][3][4] The diagnosis is easily established by complete history and radiological findings. The histopathological examination analyses are the important tool to qualify and quantify the tumor according to the histopathological parameters that enable differential diagnosis of the neoplasm.…”

Section: Introductionmentioning

confidence: 99%

Histomorphological spectrum of Leiomyoma – a one year retrospective study

Baral¹,

Shrestha²

2021

Nepal J Obstet Gynaecol

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Aims: To analyze the histomorphology of leiomyoma in specimens received in Pathology. Methods: This is a retrospective descriptive study of histopathology database of histomorphologic spectrum of leiomyoma at Pathology Laboratory of Nobel Medical College Teaching Hospital, Biratnagar, Nepal from April 2020 to April 2021. Data for the leiomyoma were analyzed descriptively. Ethical approval was obtained from the Institutional review committee. Results: A total of 1705 histopathology specimens were received in one year from April 2020 to April 2021 out of which 620 (37%) were from the Department of Gynecology and Obstetrics for histopathological analysis of various specimens. Total of 106 specimens of hysterectomy and myomectomy were included. Maximum number of leiomyoma was seen in the body 48 (45%) followed by fundus 34 (32%) and one from the lateral wall of the vagina 1 (0.9%). Mean age was 42 (24-70) years. Maximum size was 35 cm and minimum was 0.5 cm in diameter. Out of the rare ones one case of STUMP, Lipoleiomyoma and Mitotically active leiomyoma each were seen. Degenerative change was in 40 cases with hyaline type as the commonest one (33%); 61% were intramural; and 12% were multiple. Presentation was lower abdominal pain and abnormal uterine bleeding in 39.6%. Conclusions: Cases of leiomyoma may present with abdominal mass, pain and bleeding but the degenerative changes and malignant transformation can't be identified without histopathological examination.

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Section: Introductionmentioning

confidence: 99%

Histomorphological spectrum of Leiomyoma – a one year retrospective study

Baral¹,

Shrestha²

2021

Nepal J Obstet Gynaecol

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“…Flake and colleagues categorized uterine leiomyoma in 4 different phases on the basis of percent collagen content ranging from none (phase 1) up to 72% (>50%, phase 4) independently of the tumor size 67 . The collagen content in myometrium ranged from 0.3% to 23% in our previous samples 11 and from 1.6% up to 20.70% in Da Silva’s study 66 . Interestingly, the leiomyoma tissues appeared inhomogeneous also in stiffness, quantified through rheometry by measuring complex shear moduli of the tissues.…”

Section: Discussionmentioning

confidence: 46%

“…We and others have recently observed that the amount of collagen inside tissues within and between biopsies is heterogeneous mainly in leiomyoma, but also in myometrial tissue. For example, morphometric analysis of Masson’s trichrome stained tissue reported values of collagen in leiomyoma ranging from 4.7% to 31,3% in our previous study 11 ; from 37% to 77% in Jayes 65 study and from 3.9% up to 70% in a large study of brazilian cases (n = 150) performed by Da Silva 66 . Flake and colleagues categorized uterine leiomyoma in 4 different phases on the basis of percent collagen content ranging from none (phase 1) up to 72% (>50%, phase 4) independently of the tumor size 67 .…”

Section: Discussionmentioning

confidence: 70%

Advanced 3D Imaging of Uterine Leiomyoma’s Morphology by Propagation-based Phase-Contrast Microtomography

Giuliani

Greco

Pacilè

et al. 2019

Sci Rep

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Uterine leiomyoma is the most common benign smooth muscle tumor in women pelvis, originating from the myometrium. It is caused by a disorder of fibrosis, with a large production and disruption of extracellular matrix (ECM). Medical treatments are still very limited and no preventative therapies have been developed. We supposed that synchrotron-based phase-contrast microtomography (PhC-microCT) may be an appropriate tool to assess the 3D morphology of uterine leiomyoma, without the use of any contrast agent. We used this technique to perform the imaging and the quantitative morphometric analysis of healthy myometrium and pathologic leiomyomas. The quantitative morphometric analysis of collagen bundles was coupled to the Roschger approach. This method, previously only used to evaluate mineralized bone density distribution, was applied here to study the fibrosis mass density distribution in healthy and pathologic biopsies from two patients. This protocol was shown to be powerful in studying uterine leiomyomas, detecting also small signs of the ECM alteration. This is of paramount importance not only for the follow-up of the present study, i.e. the investigation of different compounds and their possible therapeutic benefits, but also because it offers new methodologic possibilities for future studies of the ECM in soft tissues of different body districts. Uterine leiomyoma (myoma or fibroid) is the most common benign smooth muscle tumor in women pelvis causing significant morbidity in a large segment of reproductive-aged women 1,2 , including significant impact on the reproductive healthy status and on the pregnancy outcome of affected patients 3,4. Fibroids remain the leading indication for hysterectomy 5,6 especially during the peri-menopause period, although the assumption that leiomyoma symptoms will resolve with the onset of the menopause is too simplistic and may not be always valid 7. Medical treatments for leiomyoma are still very limited and no preventative therapies have been developed 8. The precise pathogenesis of uterine leiomyoma is not well understood. Genetic alterations, epigenetic mechanisms, steroids, growth factors, cytokines and chemokines provide the clue of initiators and promoters of leiomyoma growth 9. Uterine myoma is thought to be a consequence of myofibroblasts activation and extracellular matrix (ECM) production, following an improper inflammatory response inside the myometrium 10-12. Excessive accumulation of ECM components including collagens, fibronectin and proteoglycans are the major structural part of leiomyoma tissue that are abnormally orientated modifying mechanical stress on cells and leading to activation of internal mechanical signaling and dynamic reciprocity 13-15. The ECM stiffness causes the rigid structure of the leiomyoma and it is thought to be a cause of abnormal bleeding and pelvic pressure and pain 16. The role of inflammation and reparative processes as well as the effect of some potential therapeutic compounds (tranilast, strawberry extracts, omega-3 fatty acids, g...

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“…Миома матки -одна из наиболее распространенных доброкачественных опухолей малого таза у женщин. Она выявляется у 20-40 % пациенток репродуктивного возраста [51]. Миома матки больших размеров, которая сдавливает окружающие ткани, нарушая циркуляцию крови в венах малого таза, также может стать причиной ТЭЛА [52,53].…”

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Features of pulmonary thromboembolism in women

2021

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Pulmonary artery embolism (PAE) is usually a diagnosis of exclusion. Verification of a more common pathology takes time, which may become critical for treatment of pulmonary embolism and saving the patient’s life. Since PAE is an acute disease, the time window for medical care largely determines the prognosis. Therefore, the differential diagnostic process should include thromboembolism already at the first visit. It is important to determine risk factors for PAE taking into account the patient’s personality and gender. Obtained data may help the physician to determine quickly the expedience of visualizing studies, such as ventilation/perfusion scintigraphy, angiopulmonography, computed tomographic angiopulmonography. For women, it is important to collect specific information, such as the presence of large uterine fibroids, use of combined oral contraceptives or hormonal replacement therapy; to ask how long ago the patient had pregnancy and delivery, whether she has thrombophilia or oncological diseases.

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Histopathological and Digital Morphometrical Evaluation of Uterine Leiomyoma in Brazilian Women

Cited by 4 publications

References 23 publications

Histomorphological spectrum of Leiomyoma – a one year retrospective study

Histomorphological spectrum of Leiomyoma – a one year retrospective study

Advanced 3D Imaging of Uterine Leiomyoma’s Morphology by Propagation-based Phase-Contrast Microtomography

Features of pulmonary thromboembolism in women

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