Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from a uranium mine pond

Marques, Sérgio M.; Antunes, Sara C.; Pissarra, Hugo; Pereira, Manuela; Gonçalves, Fernando; Pereira, Ricardo

doi:10.1016/j.aquatox.2008.04.010

Cited by 59 publications

(36 citation statements)

References 46 publications

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“…Con respecto a la morfología de los hematíes, se determinó la frecuencia de macrocitos (MA), microcitos (MI), hematíes policromatófilos (PO) e hipocrómicos (HY). Se calculó la frecuencia de eritrocitos inmaduros, como medida indirecta de la hematopoyesis, a través de la cantidad de eritroblastos y PO (Marques et al 2009). También se consignaron las alteraciones nucleares que pudieran presentarse en los hematíes: núcleo arriñonado o escotado (KN) o "mellado" (EN), presencia de lóbulos (LN), micronú-cleos (MN) y células binucleadas (BN), con las que se calculó la frecuencia total de alteraciones nucleares (FAN=KN+EN+LN+MN+BN).…”

Section: Morfología Sanguíneaunclassified

“…A pesar de esto, se ha propuesto que se consideren como alteraciones celulares producidas como respuesta a los agentes generadores de estrés (Çava & Ergene-Gözükara 2003, Barni et al 2007. En anfibios adultos, ha sido utilizado como marcador de genotoxicidad en Pelophylax perezi (Rana perezi Seoane, 1885) expuestas a efluentes de minas (Marques et al 2009).…”

Section: Cuadro 3 Puntaje De Las Reacciones Citoquímicasunclassified

“…Asimismo, las variaciones en la morfología eritrocítica se asocian con exposición de aminas aromáticas, metales y estrés (Krauter 1993, Perí et al 1998, Chiesa et al 2006, Barni et al 2007. Las alteraciones nucleares, como la presencia de micronúcleos, son utilizadas como marcadores de daño cromosómico (Békaert et al 2002, Huanga et al 2007, Marques et al 2009). Por otro lado deben considerarse distintos factores, como la variabilidad del cuadro hemático a lo largo del año y la edad de los individuos, ya que características citológicas normales podrían ser confundidas con procesos patológicos o respuestas a modificaciones del ambiente (varela & Sellares 1937a, Arserim & Mermer 2008, Marques et al 2009).…”

unclassified

“…Las alteraciones nucleares, como la presencia de micronúcleos, son utilizadas como marcadores de daño cromosómico (Békaert et al 2002, Huanga et al 2007, Marques et al 2009). Por otro lado deben considerarse distintos factores, como la variabilidad del cuadro hemático a lo largo del año y la edad de los individuos, ya que características citológicas normales podrían ser confundidas con procesos patológicos o respuestas a modificaciones del ambiente (varela & Sellares 1937a, Arserim & Mermer 2008, Marques et al 2009). El objetivo de este trabajo fue obtener datos hematológicos y citoquímicos basales para Rhinella fernandezae, Gallardo 1957, especie ampliamente distribuida en Uruguay, noreste de Argentina, sur de Brasil y Paraguay (IUCN 2009) y cuyas poblaciones son frecuentes en los agroecosistemas argentinos (Peltzer et al 2007).…”

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Hematología y citoquímica de las células sanguíneas de Rhinella fernandezae (Anura: Bufonidae) en Espinal y Delta-Islas del río Paraná, Argentina

Zenklusen¹,

Attademo²,

Peltzer³

et al. 2010

RBT

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Recibido 12-v-2010.Corregido 15-vIII-2010. Aceptado 17-IX-2010.Abstract: Hematology and blood cell cytochemistry of Rhinella fernandezae (Amphibia: Anura) from Espinal and Delta-Islands of Paraná River, Argentina. The description of amphibian hematology is scarce and most of these studies have been done in species from North America, Asia and Europe. With the purpose to obtain basic hematological information of Rhinella fernandezae, 23 blood samples from Santa Fe and Entre Ríos natural reserves were studied. Blood of each individual was extracted by cardiac puncture and hemograms were carried out. Morphological and cytochemical description of blood cells were analyzed in slides and were inspected for extra and intra cellular parasites. Five leucocytes types were observed, being lymphocytes the predominant ones followed by basophiles. Heterophils and eosinophils were positive to PAS, Sudan B and peroxidase. The erythrocytes and its precursors were negative for cytochemical reactions. Micronuclei and nuclear alterations frequencies were scarce. No significant differences (p>0.05) were observed between sexes neither in hemograms nor in blood cells morphology. Microfilarias were the only hemoparasites found with a relative low prevalence and infection intensity. The hematological characteristics studied were similar to those reported for other amphibians, suggesting that R. fernandezae individuals present optimal nutritional and immunological status. Rev. Biol. Trop. 59 (1): 17-28. Epub 2011 March 01.

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Section: Morfología Sanguíneaunclassified

Section: Cuadro 3 Puntaje De Las Reacciones Citoquímicasunclassified

unclassified

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Hematología y citoquímica de las células sanguíneas de Rhinella fernandezae (Anura: Bufonidae) en Espinal y Delta-Islas del río Paraná, Argentina

Zenklusen¹,

Attademo²,

Peltzer³

et al. 2010

RBT

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“…The analyses of these types of damage using peripheral blood (erythrocyte) cells instead of tissues is highly useful, since peripheral blood cells are considered sensitive -as is the case of kidney cells, the main organ responsible for blood cell formation in fish, besides which they are easier to sample [10]. Nuclear abnormalities in erythrocytes (NAEs), such as the presence of micronuclei (MNs), are recognized as important genotoxic biomarkers [11,12]. MNs are particularly responsible for the clastogenic and an eugenic compounds in the aquatic environment and can indicate latent risk to the biota Hoshina et al [11,].…”

Section: Introductionmentioning

confidence: 99%

Trace Elements and Nuclear Abnormalities in Fish Species of Two Brazilianestuarine Regions – An Attempt to Increase the Matrix for Environmental Monitoring

ES¹

2017

JAMB

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Trace elements As, Co, Fe, Hg, Se, Zn and Cr were determined in muscle tissue, gills and liver and nuclear abnormalities in erythrocytes (NAEs) were observed in 7 fish species (Chaetodipterusfaber, Paralanchurusbrasiliensis, Micropogoniasfurnieri, Achiruslineatus, Genyatremusluteus, Menticirrhus Americanos and Micropogoniasfurnieri), from two estuarine regions subject to different levels of anthropogenic impact (preserved and polluted) to provide information on contamination levels. The bioaccumulation in C. faber suggested that it should be proposed as a bioindicator of pollution in estuaries. The hydrological data: temperature, salinity and dissolved oxygen characterized the estuarine waters. A paradoxical result relating to As, Co, Fe and Zn concentrations was that, in general, these were higher in the fish species of the Cananeia estuary (a preserved area), while Hg concentrations and NAEs were higher in the species of the Santos-São Vicente estuary (SSV) (the polluted area). The bioaccumulation in tissue-organs decreased in the following order: liver>gill>muscle, with the exception of Hg that was liver>muscle with no detection in gills. Concerning the genotoxic effects, C. faber showed a more significant NAE, besides clastogenic effects, demonstrated by the greater frequency of micronuclei. Differences found between the fish species could be related to their feeding habits and to salinity. This is a contribution to making fishery efforts more profitable and enhancing the bioindicator bank.

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Threatened aquatic Andean frogs and mining activity in the Lithium Triangle of South America: Can both coexist?

Salica,

Gastón,

Akmentins

et al. 2023

Aquatic Conservation

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The aquatic Andean frogs of genus Telmatobius are one of the most threatened amphibian taxa, with many species having suffered drastic declines and even possible extinction. The Laguna de los Pozuelos' rusted frog Telmatobius rubigo is a fully aquatic species that inhabits lotic systems in the Argentinean vertex of the Lithium Triangle of South America. This study estimated the environmental suitability of an area and geographical distribution of T. rubigo through an ecological niche model. This study also aimed to assess the possible extent of impacts related to mining operations on this threatened species. The ecological niche model of T. rubigo was obtained using specific freshwater habitat variables and the MaxEnt algorithm. The possible extent of impacts related to mining operations was evaluated by overlaying the mining tenure and the estimated distribution of T. rubigo resulting from the model. According to the ecological niche models, T. rubigo could occupy 2,001 km2 in four Argentine basins and three hydrographical units of southern Bolivia and is unlikely to occur in northern Chile. Five environmental variables had the highest contribution to the best selected model: shrub coverture, soil pH, soil organic carbon, and two variables related to the upstream air temperature. The suitable area for T. rubigo overlaps with mining operations by 61.8%. We advise the local communities, governmental environmental agencies, mining companies, and environmental assessors to consider the presence of threatened Telmatobius species in areas subject to mining to protect them and their environment. We encourage incorporating the aquatic Andean frogs as key species into management, environmental monitoring, and remediation plans.

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Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from a uranium mine pond

Cited by 59 publications

References 46 publications

Hematología y citoquímica de las células sanguíneas de Rhinella fernandezae (Anura: Bufonidae) en Espinal y Delta-Islas del río Paraná, Argentina

Hematología y citoquímica de las células sanguíneas de Rhinella fernandezae (Anura: Bufonidae) en Espinal y Delta-Islas del río Paraná, Argentina

Trace Elements and Nuclear Abnormalities in Fish Species of Two Brazilianestuarine Regions – An Attempt to Increase the Matrix for Environmental Monitoring

Threatened aquatic Andean frogs and mining activity in the Lithium Triangle of South America: Can both coexist?

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