Histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in Poland

Damek-Poprawa, Monika; Sawicka-Kapusta, K.

doi:10.1016/j.envres.2004.02.003

Cited by 114 publications

(60 citation statements)

References 23 publications

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“…In the present study, no genotoxic effects were observed after exposure to Sinos substances in the Sinos River. Most data concerning the toxic effects of pollutants on the structure and function of organs come from experiments in laboratory animals exposed to a single compound, such as heavy metals (Damek-Poprawa and Sawicka-Kapusta, 2004;Koyu et al, 2006), and less frequently to complex mixtures, such as rivers. Contaminated rivers often contain a wide variety of organic and inorganic pollutants, including potentially toxic metals such as Pb, Cd, Fe, Zn, Cu, Mn, Mo, and Cr (Sánchez-Chardi et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

et al. 2010

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The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver.Keywords: genotoxicity, pollution, biomarkers, oxidative stress, tissue damage. Análise de múltiplos biomarcadores em ratos para determinar o impacto da poluição do rio dos SinosResumo O objetivo deste estudo foi determinar a viabilidade de combinar a análise da qualidade da água com o estudo de biomarcadores para caracterizar a relação entre contaminação antropogênica e resposta biótica no rio dos Sinos, sul do Brasil. Ratos Wistar machos foram estudados usando três biomarcadores combinados com análises físicas, químicas e microbiológicas para determinar os efeitos da poluição em quatro locais de amostragem de água. A indução do estresse oxidativo foi quantificada pelos níveis de MDA no sangue periférico, o dano do DNA linfocitário foi determinado usando o ensaio cometa, e as alterações histopatológicas foram analisadas no fígado. As amostras da água do rio dos Sinos (dos quatro pontos selecionados) foram administradas por via oral (como água para beber) por 48 horas. Nenhum aumento do estresse oxidativo ou do dano do DNA foi observado. Entretanto, dano do tecido hepático foi verificado nos animais expostos à ingestão das amostras da água, indicando que o rio dos Sinos está contaminado com substâncias hepatotóxicas. As análises das amostras de água confirmaram a redução da qualidade da água em direção à foz do rio.Palavras-chave: genotoxicidade, poluição, biomarcadores, estresse oxidativo, dano tecidual.

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Section: Discussionmentioning

confidence: 99%

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

et al. 2010

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“…Many metals can be mutagenic Hei and Filipic 2004), carcinogenic (Waalkes 2003;Waisberg et al 2003), teratogenic (Calevro et al 1998), and can also alter the activity of enzymes, transport proteins, cell and tissue structure and functions (Wlostowski et al 2000;Yeh et al 2000;Damek-Poprawa and Sawicka-Kapusta 2004). Trace metals may also induce apoptosis (Lin et al 2003;Wätjen and Beyersmann 2004;Banasik et al 2005;Xu et al 2006) and metallothionein expression (Bracken and Klaassen 1987;Jeffery et al 1987;Huang et al 2007) in several tissues and organs.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis, metallothionein, and bioavailable metals in domestic mice (Mus musculus L.) from a human-inhabited volcanic area

et al. 2007

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The influence of extreme environments of volcanic origin over vertebrates and the cellular responses that these may give are almost unknown. The main objectives were to evaluate the exposure of mice to metals in the interior of houses of a small village settled inside a volcanic crater (Furnas, Azores), and the levels of apoptosis and metallothionein in the organs (lung, liver, and kidney) of those animals. Adult mice (Mus musculus) were captured in two areas, one with volcanic activity and the other without it over the last three centuries. In the excised organs, analysis of metals (Al, Cd, Pb, Zn), TUNEL assay for apoptosis, and immunohistochemistry for metallothionein were undertook. Mice from the area with volcanic activity presented higher levels of apoptosis and metallothionein than those from the area without volcanic activity. Such results were in agreement with the differences in metal burdens of the three organs, and interestingly these concentrations were similar to or higher than others found in heavily polluted areas outside the Azores. Thus, there may be a high risk of harmful effects for organisms, including humans, inhabiting areas with volcanism, where hazardous gases and metals in the air are very common during the entire day or even all year round.

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“…17 However, after the coating of PGA, the pH value declined to 2.4 amid the presence of abundant carboxylic acid groups. The low pH PZC of PGA-SPIONs indicated that their negative surface charges at most of the environmental pH (3-9) and biological pH (1)(2)(3)(4)(5)(6)(7)(8) should prevent aggregation and facilitate the …”

Section: Magnetic Propertymentioning

confidence: 99%

“…This variation in removal capacity may be due to differences in electronegativity between lead (2.33) and cadmium (1.69). It is also possible that the high affinity of several labile hydrolysis and polymeric species formed by lead at high concentrations (such as [Pb 4 base through covalent interactions. Given the classification of lead as a borderline acid and cadmium as a soft acid, their interaction with a hard base like carboxylate anions on PGA may not be possible unless through contribution from some other functional groups like amides, as pointed out by Siao et al 24 …”

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confidence: 99%

In vitro removal of toxic heavy metals by poly(γ-glutamic acid)-coated superparamagnetic nanoparticles

Inbaraj¹,

Chen²

2012

IJN

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Background: Chelation therapy involving organic chelators for treatment of heavy metal intoxication can cause cardiac arrest, kidney overload, mineral deficiency, and anemia. Methods: In this study, superparamagnetic iron oxide nanoparticles (SPIONs) modified with an edible biopolymer poly(γ-glutamic acid) (PGA) were synthesized by coprecipitation method, characterized and evaluated for their removal efficiency of heavy metals from a metal solution, and simulated gastrointestinal fluid (SGIF). Results: Instrumental characterization of bare-and PGA-SPIONs revealed 7% coating of PGA on SPIONs with a spherical shape and an iron oxide spinel structure belonging to magnetite. The particle sizes as determined from transmission electron microscopy images were 8.5 and 11.7 nm for bare-and PGA-SPIONs, respectively, while the magnetization values were 70.3 and 61.5 emu/g. Upon coating with PGA, the zeta potentials were shifted from positive to negative at most of the environmental pH (3-8) and biological pH (1-8), implying good dispersion in aqueous suspension and favorable conditions for heavy metal removal. Batch studies showed rapid removal of lead and cadmium with the kinetic rates estimated by pseudo-second-order model being 0.212 and 0.424 g/mg⋅min, respectively. A maximum removal occurred in the pH range 4-8 in deionized water and 5-8 in SGIF corresponding to most gastrointestinal pH except for the stomach. Addition of different ionic strengths (0.001-1 M sodium acetate) and essential metals (Cu, Fe, Zn, Mg, Ca, and K) did not show any marked influence on lead removal by PGASPIONs, but significantly reduced the binding of cadmium. Compared to deionized water, the lead removal from SGIF was high at all pH with the Langmuir monolayer removal capacity being 98.70 mg/g for the former and 147.71 mg/g for the latter. However, a lower cadmium removal capacity was shown for SGIF (23.15 mg/g) than for deionized water (31.13 mg/g). Conclusion: These results suggest that PGA-SPIONs could be used as a metal chelator for clinical treatment of metal poisoning.

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Histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in Poland

Cited by 114 publications

References 23 publications

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

Apoptosis, metallothionein, and bioavailable metals in domestic mice (Mus musculus L.) from a human-inhabited volcanic area

In vitro removal of toxic heavy metals by poly(γ-glutamic acid)-coated superparamagnetic nanoparticles

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Histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in Poland

Cited by 114 publications

References 23 publications

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

Apoptosis, metallothionein, and bioavailable metals in domestic mice (Mus musculus L.) from a human-inhabited volcanic area

In vitro removal of toxic heavy metals by poly(&gamma;-glutamic acid)-coated superparamagnetic nanoparticles

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In vitro removal of toxic heavy metals by poly(γ-glutamic acid)-coated superparamagnetic nanoparticles