Histopathological Changes in the Reproductive Organs of Manchego Ewes Grazing on Lucerne

Cantero, Antonio; Sancha, J. L.; Flores, Juana M.; Rodrı́guez, Ana Valdeolmillos; González, Teresa Artola

doi:10.1111/j.1439-0442.1996.tb00459.x

Cited by 22 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[23] Coumestrol, a coumestan, is found in alfalfa. [24] Genistein, daidzein, and equol (5, 50 µM) decreased (P < 0.05) T secretion by incubated gander Leydig cells (Fig. 1).…”

Section: Experiments 1: Interaction Of Pe With Ermentioning

confidence: 89%

Mechanism of phytoestrogen action in Leydig cells of ganders (Anser anser domesticus): Interaction with estrogen receptors and steroidogenic enzymes

Opałka

Kamińska

Leska

et al. 2012

Journal of Environmental Science and Health, Part A

View full text Add to dashboard Cite

Phytoestrogens (PE) are plant-derived compounds that have an estrogen-like activity and they can influence male and female reproduction. The possible mechanisms of PE action may be including: the binding to estrogen receptors (ER) and the interaction with the key steroidogenic enzymes. The aim of this study was to investigate if PE has effect on steroidogenesis of gander testicular cells by above-described pathways. The Leydig cells were isolated from testes of White Kołuda ganders at the peak of their reproductive activity (March). These Leydig cells (1 × 10(5)per mL) were pre-incubated with the ER inhibitor - ICI 182, 780 (100 nM) for 3 h and then these cells were incubated with PE (5 and 50 μM): genistein, daidzein, equol and coumestrol during next 20 h or untreated control and the Leydig cells that were previously treated (20 h) with genistein (5 and 50 μM) were incubated for next 6 h with steroid intermediates (20 μM) as testosterone (T) precursors: hydroxycholesterol, pregnenolone, progesterone and androstenedione. Concentrations of T in the samples of incubation medium were measured using radioimmunoassay. Genistein, daidzein, and equol (5 and 50 μM) decreased (P < 0.05) T secretion by incubated gander Leydig cells and ICI 182, 780 did not eliminate the inhibitory effect of these PE. After genistein (50 μM) treatment, basal and stimulated with 22R-hydroxycholesterol, pregnenolone, progesterone and androstenedione, T production by testicular cells was decreased (P < 0.05). In contrast, genistein at lower dose (5 μM) did not affect the stimulatory effects of testosterone precursors. In conclusion, the inhibition of testosterone secretion by the phytoestrogens in gander Leydig cells did not depend on estrogen receptors. The suppression of steroidogenesis in these cells may be in part conducted by interaction of phytoestrogens with key steroidogenic enzymes. However, further studies are required to elucidate the phytoestrogen mechanism of action in gander testicular cells.

show abstract

“…[23] Coumestrol, a coumestan, is found in alfalfa. [24] Genistein, daidzein, and equol (5, 50 µM) decreased (P < 0.05) T secretion by incubated gander Leydig cells (Fig. 1).…”

Section: Experiments 1: Interaction Of Pe With Ermentioning

confidence: 89%

Mechanism of phytoestrogen action in Leydig cells of ganders (Anser anser domesticus): Interaction with estrogen receptors and steroidogenic enzymes

Opałka

Kamińska

Leska

et al. 2012

Journal of Environmental Science and Health, Part A

View full text Add to dashboard Cite

show abstract

“…Equol is exclusively a product of intestinal bacterial biotransformation of dietary daidzein (Setchell et al, 2002). Coumestrol is the best known coumestan with the highest oestrogenic potency (Whitten and Patisaul, 2001), but a significant source of this phytoestrogens for animals is alfalfa (Cantero et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Opałka¹,

Kamińska²,

Piskuła

et al. 2006

British Poultry Science

View full text Add to dashboard Cite

The aim of this study was to examine ganders at different stages of the reproductive season and the effect of: (1) diets with high phytoestrogen content and (2) in vitro phytoestrogen treatment on testosterone secretion by isolated Leydig cells. Thirty-six male Biłgoraj geese were fed control diets with low phytoestrogen content (containing grass meal) and diets with high phytoestrogens (containing alfalfa meal and soy). Testes were obtained from both groups of ganders at three different times of the breeding season: peak of reproductive activity (March), second half of reproductive activity (May) and beginning of photorefractoriness (July). Isolated Leydig cells were incubated with LH as well as genistein, daidzein, equol and coumestrol and the concentration of testosterone in the medium was determined by radioimmunoassay. The mean weight of testes from ganders in May and July decreased relative to their weights in March, but no significant differences among experimental groups were noted. No differences were observed in basal and LH-stimulated testosterone secretion by Leydig cells of ganders fed the control diets and the diets with higher phytoestrogen content. In July, LH did not stimulate testosterone secretion in either group. In vitro treatment with genistein, daidzein and equol (5 and 50 microM) inhibited basal and LH-stimulated testosterone production by Leydig cells from both groups. Coumestrol (5 and 50 microM) inhibited basal testosterone secretion only in March in the control group. Dietary exposure to phytoestrogens had a slight effect on in vitro testicular secretion in ganders. In vitro treatment with phytoestrogen inhibited testosterone production by Leydig cells. Genistein showed the strongest effect and coumestrol had the weakest influence on testicular secretion.

show abstract

“…Alterations were especially noted in the uterus (greater than normal development of the cervical folds); prolonged exposure to lucerne led to permanent effects on the reproductive organs [119]. Coumestans can produce an oestrogenic syndrome; lambs fed lucerne containing coumestrol (119 cf.…”

Section: Coumestan Infertilitymentioning

confidence: 99%

Fertility of Herbivores Consuming Phytoestrogen-containing Medicago and Trifolium Species

Reed¹

2016

Agriculture

View full text Add to dashboard Cite

Despite their unrivalled value in livestock systems, certain temperate, pasture, legume species and varieties may contain phytoestrogens which can lower flock/herd fertility. Such compounds, whose chemical structure and biological activity resembles that of estradiol-17α, include the isoflavones that have caused devastating effects (some of them permanent) on the fertility of many Australian sheep flocks. While the persistence of old 'oestrogenic' ecotypes of subterranean clover (Trifolium subterraneum) in pasture remains a risk, genetic improvement has been most effective in lowering isoflavone production in Trifolium species; infertility due to 'clover disease' has been greatly reduced. Coumestans, which can be produced in Medicago species responding to stress, remain a potential risk in cultivars susceptible to, for example, foliar diseases. In the field, coumestrol is often not detected in healthy vegetative Medicago species. Wide variation in its concentration is influenced by environmental factors and stage of growth. Biotic stress is the most studied environmental factor and, in lucerne/alfalfa (Medicago sativa), it is the major determinant of oestrogenicity. Concentrations up to 90 mg coumestrol/kg (all concentrations expressed as DM) have been recorded for lucerne damaged by aphids and up to 600 mg/kg for lucerne stressed by foliar disease(s). Other significant coumestans, e.g., 4'-methoxy-coumestrol, are usually present at the same time. Concentrations exceeding 2000 mg coumestrol/kg have been recorded in diseased, annual species of Medicago. Oestrogenicity of some Medicago species is also influenced by maturity and senescence. Studies in Israel, North America, Europe, New Zealand and Australia have confirmed that coumestans in lucerne, represent an acute or sub-acute loss of reproductive efficiency in herbivores, e.g., sheep, cattle, and possibly horses. When sufficiently exposed peri-conception, coumestrol, sometimes present in lucerne, be it as pasture, hay, silage, pellets, meal, and sprouts, is associated with what can be an insidious, asymptomatic, infertility syndrome. Most livestock research with oestrogenic lucerne has been conducted with sheep. Ewes may be at risk when the coumestrol concentration in their diet exceeds 25 mg/kg. In studies where lambing was compared for lucerne and a phytoestrogen-free treatment, the mean decrease in lambs born/ewe was 13%; ewes on lucerne, exhibited a lower frequency of multiple births.

show abstract

Histopathological Changes in the Reproductive Organs of Manchego Ewes Grazing on Lucerne

Cited by 22 publications

References 13 publications

Mechanism of phytoestrogen action in Leydig cells of ganders (Anser anser domesticus): Interaction with estrogen receptors and steroidogenic enzymes

Mechanism of phytoestrogen action in Leydig cells of ganders (Anser anser domesticus): Interaction with estrogen receptors and steroidogenic enzymes

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Fertility of Herbivores Consuming Phytoestrogen-containing Medicago and Trifolium Species

Contact Info

Product

Resources

About