Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice

Heidarpour, Mojgan; Ennaifer, Emna; Ahari, Hamed; Srairi-Abid, Najet; Borchani, Lamia; Khalili, Ghader; Amini, Hossein; Anvar, Amir Ali; Boubaker, Samir; Elayeb, Mohamed; Shahbazzadeh, Delavar

doi:10.1016/j.toxicon.2011.12.011

Cited by 38 publications

(13 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Which have been observed by several authors, indeed, intestine and stomach suffer from envenomation by H. lepturus (Heidarpour et al, 2012). Such alteration was also observed after injection of brown spiders (Loxosceles genus) venom (Babcock et al, 1981).…”

Section: Discussionmentioning

confidence: 54%

“…Substantial evidences support this view. In fact, histopathological studies of H. lepturus envenomation showed massive cardiac myocytolysis with interstitial necrosis (Heidarpour et al, 2012), and after injection of sublethal doses of T. serrulatus venoms, which induce Hemorrhage, ruptured and hypertrophied fibers in heart (Corrêa et al, 1997). Acute myocarditis was also observed after accidental envenomation of humans by the venom of Tityus trinitatis (Daisley et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Hidan

Touloun

Hiba

et al. 2015

Toxicon

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Hidan

Touloun

Hiba

et al. 2015

Toxicon

View full text Add to dashboard Cite

“…This survival strongly affirms that all mice were completely protected against wide range of different lethal dosages of crude venom (10 LD 100 , equal to 50 mg/Kg), thus illustrating a successful and 100% immunization in the mice. Heidarpour et al determined the LD 50 amount of H. lepturus crude venom as 5 mg/Kg in BALB/c mice [36]. These data are greatly in accordance with ELISA in which a raised antibody titer was observed at the first injection of mut-rPLD1 followed by reaching the plateau state from the 3rd injection to the 6th injection.…”

Section: Discussionmentioning

confidence: 58%

Novel Mutant Phospholipase D from Hemiscorpius lepturus Acts as A Highly Immunogen in BALB/c Mice Against the Lethality of Scorpion Venom

et al. 2020

Self Cite

View full text Add to dashboard Cite

Hemiscorpius lepturus (H. lepturus) which belongs to the Scorpionidae family, is the deadliest scorpion in Iran. It causes pathological manifestations like dermonecrosis, hemolysis, renal failure, necrotic ulcers, and in some cases, even death. The venom of this scorpion is well-known for its cytotoxic effects in comparison with the other venomous scorpions which show significant neurotoxic effects. Due to the painless nature of the sting of this scorpion, the clinical symptoms occur in victims 24 to 72 h post-sting. In our previous studies during the last decade, we demonstrated that the medical complications are attributable to the presence of phospholipase D (PLD) as a major toxin in the venom. With the purpose of designing and constructing a vaccine against H. lepturus for humans, animal model experiments were performed. To achieve this goal, non-toxic PLD was developed by mutation of two critical catalytic residues—His12 and His48—into alanines and the product was then denominated mut-rPLD1. The in-vivo tests showed that the mice immunized with interval doses of 10 µg of mut-rPLD1, were completely protected against 10× the LD100 of the venom. In conclusion, this mutant may be an effective vaccine candidate against scorpion envenomation by H. lepturus in future clinical studies.

show abstract

“…However, in addition, immunopathologic changes have been observed, with accompanying tubular necrosis in mice injected with Androctonus australis hector scorpion venom [8]. Tubular necrosis has also been observed following envenomation with Hemiscorpius Lepturus scorpion venom [22, 23]. However, it is unclear whether the renal injury resulting from scorpion venoms is due to a direct effect of the venom components, or a whether renal injury depends upon the catecholamine storm and/or the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

In vitro studies with renal proximal tubule cells show direct cytotoxicity of Androctonus australis hector scorpion venom triggered by oxidative stress, caspase activation and apoptosis

Saidani

Hammoudi-Triki

Laraba-Djebari

et al. 2016

Toxicon

View full text Add to dashboard Cite

Scorpion envenomation injures a number of organs, including the kidney. Mechanisms proposed to explain the renal tubule injury include direct effects of venom on tubule epithelial cells, as well as indirect effects of the autonomic nervous system, and inflammation. Here, we report direct effects of Androctonus australis hector (Aah) scorpion venom on the viability of Renal Proximal Tubule (RPT) cells in vitro, unlike distal tubule and collecting duct cells. Extensive NucGreen nuclear staining was observed in immortalized rabbit RPT cells following treatment with Aah venom, consistent with cytotoxicity. The involvement of oxidative stress is supported by the observations that 1) anti-oxidants mitigated the Aah venom-induced decrease in the number of viable RPT cells, and 2) Aah venom-treated RPT cells were intensively stained with the CellROX® Deep Red reagent, an indicator of Reactive Oxygen Species (ROS). Relevance to normal RPT cells is supported by the red fluorescence observed in Aah venom treated primary rabbit RPT cell cultures following their incubation with the Flica reagent (indicative of caspase activation and apoptosis), and the green fluorescence of Sytox Green (indicative of dead cells).

show abstract

Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice

Cited by 38 publications

References 27 publications

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Behavioral, histopathological and biochemical impairments observed in mice envenomed by the scorpion: Hottentota gentili (Pallary, 1924)

Novel Mutant Phospholipase D from Hemiscorpius lepturus Acts as A Highly Immunogen in BALB/c Mice Against the Lethality of Scorpion Venom

In vitro studies with renal proximal tubule cells show direct cytotoxicity of Androctonus australis hector scorpion venom triggered by oxidative stress, caspase activation and apoptosis

Contact Info

Product

Resources

About