Histopathological evaluation of Atlantic tomcod (Microgadus tomcod) collected at estuarine sites receiving pulp and paper mill effluent

Couillard, Catherine M.; Williams, P. James; Courtenay, Simon C.; Rawn, G. P.

doi:10.1016/s0166-445x(98)00085-x

Cited by 36 publications

(29 citation statements)

References 26 publications

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“…Morphological signs of exposure to chemical stress, resulting in hepatic melanomacrophage centers, free melanomacrophages and necrosis, were frequent in both reference and contaminated sites, either before or after the VOS (Table 3). Melanomacrophage centers in liver are related to a higher phagocytotic activity, as part of the immune response to tissue lesions in exposed organisms (Couillard et al 1999, Oliveira Ribeiro et al 2005, Rabitto et al 2005. Hemorrhage foci have been found only in the contaminated site after VOS (Table 2).…”

Section: Discussionmentioning

confidence: 99%

An assessment of acute biomarker responses in the demersal catfish Cathorops spixii after the Vicuña Oil Spill in a harbour estuarine area in Southern Brazil

Katsumiti

Domingos

Azevedo

et al. 2008

Environ Monit Assess

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The Vicuña oil tanker exploded in Paranaguá Bay (South of Brazil), during methanol unloading operations in front of Paranaguá Harbour, on November 15th, 2004, releasing a large amount of bunker oil and methanol. Two weeks after the accident, the acute effects of the Vicuña Oil Spill (VOS) were evaluated in the demersal catfish Cathorops spixii, comparing a contaminated (at the spill site) and a reference site inside the Bay. Data were compared to previous measurements, taken before the accident, in the same species, from the same sites. The physiological biomarkers were the ones that best reflected acute effects of the spill: plasma osmolality, chloride, calcium, magnesium, and potassium. Morphological (liver and gill histopathology) and genetic (piscine micronucleus and DNA strand breaks) biomarkers revealed that damage was already present in fishes from both reference and contaminated sites inside the Bay. Thus, the reference site is not devoid of contamination, as water circulation tends to spread the contaminants released into other areas of the Bay. Acute field surveys of oil spill effects in harbour areas with a long history of contamination should thus be viewed with caution, and whenever possible previous evaluations should be considered for proper appraisal of biomarker sensitivity, especially in mobile bioindicators such as fish.

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Section: Discussionmentioning

confidence: 99%

An assessment of acute biomarker responses in the demersal catfish Cathorops spixii after the Vicuña Oil Spill in a harbour estuarine area in Southern Brazil

Katsumiti

Domingos

Azevedo

et al. 2008

Environ Monit Assess

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“…Increase in GSI is associated with advanced maturity of testis. Couillard et al, 1999 USA, California, BKME site…”

Section: Atlantic Tomcod (Microgadus Tomcod)mentioning

confidence: 99%

Effects of Endocrine Disrupters on Sexual, Gonadal Development in Fish

Scholz¹,

Klüver²

2009

Sex Dev

120

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Steroid sex hormones play an important role in the sexual differentiation of fish. Thus, it is not surprising that chemical contaminants with steroid-like activities were considered as responsible for the unusual occurrence of gonadal intersex conditions and other gonadal aberrations in feral fish. In this review, we give an overview about field data and summarise and categorise experimental evidence that links disruption of gonadal development in gonochoristic fish to contaminations by endocrine disrupting chemicals. A comprehensive overview on laboratory studies using water-borne exposures and histopathological analysis is given. Parameters ranging from simple quantitative characteristics such as sex ratio, number of sex reversed fish, and gonadosomatic index (GSI) to detailed morphometric analyses have been considered. Categorisation of the data indicates 2 major groups of chemicals with apparently conserved effects across species, i.e. estrogenic/anti-androgenic or androgenic/anti-estrogenic compounds. Since gross morphological parameters and histological analysis are often the first parameters measured in field campaigns for sampling of feral fish, the review supports the critical evaluation of present and future field studies and the confirmation or rejection of causative links to exposure with endocrine disrupting chemicals. Furthermore, in combination with the analysis of molecular endpoints the processed data will be useful to deduce mechanistic information on potential endocrine disrupting compounds.

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“…aggregations of MAs, EGCs and epithelioid cells) at the interface between host and parasite. The MAs were observed in histological sections of 14 powan hearts, and their proliferation has been frequently associated with both physiological and pathological factors including starvation, aging, intoxication and the presence of infectious disease (Vogelbein et al 1987, Couillard & Hodson 1996, Couillard et al 1999. The presence of MAs have been reported for Rivulus marmoratus (Poey) experimentally infected with the protozoan Calyptospora funduli Overstreet, Hawkins & Fournie, 1984(Vogelbein et al 1987) and also for Gasterosteus aculeatus infected with the microsporean Glugea anomala (Dezfuli et al 2004).…”

Section: Resultsmentioning

confidence: 96%

“…During the present study, aggregates of macrophages (MAs) were noted within the fibroconnective capsule surrounding the metacercariae of I. erraticus. Although there is no accepted view regarding the role of MAs in fishes, their proliferation is frequently associated with a variety of pathological and physiological factors (Vogelbein et al 1987, Wolke 1992, Couillard et al 1999. This paper therefore attempts to establish a relationship between the MAs and the digenean larvae on heart tissue, and comments on the histopathology and ultrastructure of the tissue at the site of the host -parasite interface.…”

Section: Introductionmentioning

confidence: 99%

Histopathology, ultrastructure and immunohistochemistry of Coregonus lavaretus hearts naturally infected with Ichthyocotylurus erraticus (Trematoda)

Dezfuli¹,

Giari²,

Simoni³

et al. 2005

Dis. Aquat. Org.

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Histopathological, ultrastructural and immunohistochemical investigations were conducted on 26 specimens of powan Coregonus lavaretus (L.) from Loch Lomond (Scotland). The hearts of all 26 powan (15 females and 11 males) investigated harboured metacercariae of the digenean trematode Ichthyocotylurus erraticus (Rudolphi, 1809). The vast majority of metacercariae were located either singly or as an aggregation of white cysts on the surface of the bulbus arteriosus. The intensity of infection ranged from 2 to 200 larvae heart -1 , although the number of metacercariae found on male powan did not exceed 13. Histochemically, the parasite cyst wall gave a strong positive reaction with periodic acid schiff (PAS) and a faint positive signal with Azan-Mallory stain. All the metacercariae cysts were embedded in a granulomatous proliferation of heart epicardium tissue, forming a reactive fibroconnective capsule around the parasite. The capsule enclosing the parasite (produced by the host's reaction to the parasite) measured 13.57 to 90.20 µm (37.43 ± 3.56) in thickness. Within the capsule wall, eosinophilic granular cells (EGCs), granulocytes, melanocytes and, in some instances, partially degenerated or vacuolated epithelioid cells were observed in close proximity to the cyst wall. Pigment-bearing macrophages were scattered throughout the granulomatous host-tissue reaction and as macrophage aggregates (MAs) within the capsules surrounding parasites. Immunohistochemical tests were applied to infected heart sections using 12 different antisera. Nerve fibres immunoreactive to bombesin, substance P (SP), and atrial natriuretic peptide (ANP) antisera were observed in close proximity to the parasite larvae. The presence of a serotonin-like substance was also observed within host immune-cells surrounding trematode cysts. Large cells of the epicardium were found to be immunoreactive to met-enkephalin and vasoactive intestinal peptide (VIP) antisera but not immunoreactive to anti-protein gene-product 9.5 (PGP9.5) sera. KEY WORDS: Trematode infection · Immunohistochemistry · Neuromodulators · Heart · Coregonus lavaretusResale or republication not permitted without written consent of the publisher

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Histopathological evaluation of Atlantic tomcod (Microgadus tomcod) collected at estuarine sites receiving pulp and paper mill effluent

Cited by 36 publications

References 26 publications

An assessment of acute biomarker responses in the demersal catfish Cathorops spixii after the Vicuña Oil Spill in a harbour estuarine area in Southern Brazil

An assessment of acute biomarker responses in the demersal catfish Cathorops spixii after the Vicuña Oil Spill in a harbour estuarine area in Southern Brazil

Effects of Endocrine Disrupters on Sexual, Gonadal Development in Fish

Histopathology, ultrastructure and immunohistochemistry of Coregonus lavaretus hearts naturally infected with Ichthyocotylurus erraticus (Trematoda)

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