Histopathology, antioxidant responses, transcriptome and gene expression analysis in triangle sail mussel Hyriopsis cumingii after bacterial infection

Yang, Qinglin; Guo, Kefan; Zhou, Xicheng; Tang, Xiaoqi; Yu, Xiaobo; Yao, Weizhi; Wu, Zhengli

doi:10.1016/j.dci.2021.104175

Cited by 26 publications

(9 citation statements)

References 62 publications

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“…Most of the 293 DAS genes mainly involved in the GO terms of binding, cell part, cellular process, catalytic activity and membrane part which correlated closely with cell function and anti-infection (Ma et al 2022). Furthermore, most of these genes positively get involved in the KEGG pathways of anti-bacterial or viral pathogens (Yang et al 2021).…”

Section: Discussionmentioning

confidence: 99%

The role of outer membrane protein A in OmpA vaccine was revealed by the analysis of alternative splicing and lncRNA after the Edwardsiella anguillarum infected the immunized European eels (Anguilla anguilla)

Guo

Chen

Wan

et al. 2023

Preprint

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Edwardsiella anguillarum is a common bacterial pathogen mainly infected cultivated eel, and outer membrane protein A (OmpA) emulsified with Freund's adjuvant was evaluated as an effective fishery vaccine. However, the role of solo OmpA in the vaccine have not been explored on the molecular mechanism. In this study, we examined the RNA-seq in the liver of European eel (Anguilla anguilla) post the challenge of E. anguillarum in eels injected with the OmpA vaccine, Freund's adjuvant, or PBS to elucidate the alternative splicing (AS) and long noncoding RNA (lncRNA) in the process of E. anguillarum infection and host anti-infection using genome-wide transcriptome. After all eels were challenged by E. anguillarum at 28 d post the injection (dpi), compared to severe pathological changes in the liver of PBS injected eels (Con_inf group), the OmpA immunized eels (OmpA_inf group) showed infiltrated lymphocytes in the liver as well as the Freund's adjuvant inoculated eels (FCIA_inf group) showed slight edema of hepatocytes and blood coagulation. Compared to the Con_inf group, the relative percent survival (RPS) of eels was 77.7% and 44.4% in OmpA_inf and FCIA_inf group. The results of DEGs and DE-transcripts showed 6 samples in the OmpA_inf and FCIA_inf groups were clustered together, which different to 3 samples in the Con_inf group. We found only 35 DEGs between OmpA_inf and FCIA_inf compare to thousands of DEGs in two compares of OmpA_inf vs Con_inf and FCIA_inf Con_inf. GO and KEGG analysis of genes targeted by 37 DE-lncRNAs in co-expression and co-location showed at least 52 GO terms and 2 KEGG pathways were enrichment. The results of AS analysis showed 293 differentially alternative splicing (DAS) genes between OmpA_inf and FCIA_inf were mainly involved in GO terms of catalytic activity, membrane part and biological regulation, and KEGG pathways of signal transduction, immune system and infection disease. Finally, the interaction between proteins expressed by DAS genes and the interaction between DE-lncRNAs and target genes were explored using Cytoscape 3.9.1. The results indicated 66 DAS gene expressed proteins formed total of 50 degrees in 20 networks, and 33 DE-lncRNAs interacted with 194 target genes formed total of 246 and 41 networks in co-expression and co-location. Taken together, after the immunization of OmpA vaccine, compared to Freund's adjuvant inoculation, higher RPS was closely related to DAS genes and DE-lncRNAs in response to E. anguillarum infection in European eels. The OmpA role in vaccine was firstly revealed through AS genes and lncRNAs, and results of this study are of great significance to the development of fishery subunit vaccines.

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Section: Discussionmentioning

confidence: 99%

The role of outer membrane protein A in OmpA vaccine was revealed by the analysis of alternative splicing and lncRNA after the Edwardsiella anguillarum infected the immunized European eels (Anguilla anguilla)

Guo

Chen

Wan

et al. 2023

Preprint

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“…Consequently, the cytoskeleton is closely linked to secretion (Kardon and Vale, 2009;Roberts et al, 2013). As discussed in the prior topics (3.1.1, 3.1.2, and 3.1.3), the main role of ciliated basophilic cells of hepatopancreas is protein secretion (Tunali and Erkan, 2008;Yang et al, 2021). In front of a bacterial challenge, basophilic cells likely secret proteins and other molecules related to mussel defense, such as humoral effectors observed in other bivalves infected with Vibrio (Ji et al, 2013;Smits et al, 2020;Yang et al, 2016).…”

Section: B)mentioning

confidence: 96%

“…Cilia-and flagella-associated proteins act on the assembly of protein complexes, such as axoneme (Feng et al, 2022). Indeed, hepatopancreatic tubules of bivalves comprise two types of cells, as follows: cylindrical-shaped acidophilic digestive cells and pyramid-shaped basophilic cells (Tunali and Erkan, 2008;Yang et al, 2021). In most bivalve species, basophilic cells have cilia on their apical surfaces and are specialized in protein secretion (Tunali and Erkan, 2008;Yang et al, 2021).…”

Section: Salmonella Entericamentioning

confidence: 99%

“…Indeed, hepatopancreatic tubules of bivalves comprise two types of cells, as follows: cylindrical-shaped acidophilic digestive cells and pyramid-shaped basophilic cells (Tunali and Erkan, 2008;Yang et al, 2021). In most bivalve species, basophilic cells have cilia on their apical surfaces and are specialized in protein secretion (Tunali and Erkan, 2008;Yang et al, 2021). According to Mirvis et al (2018) and Smith et al, (2020), actin can promote cilia disassembly.…”

Section: Salmonella Entericamentioning

confidence: 99%

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The first use of LC-MS/MS proteomic approach in the brown mussel Perna perna after bacterial challenge: Searching for key proteins on immune response

Santos

Neves

Bernay

et al. 2023

Fish & Shellfish Immunology

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“…Despite the unarguable accomplishments of these early molecular studies, the availability of large-scale and more biologically informative genomics resources is almost inexistent, not only for U. delphinus but also for all freshwater mussels. In fact, for approximately 1000 known species, only four whole genome assemblies [25][26][27][28] and less than 20 transcriptomes are currently available [29][30][31][32][33][34][35][36][37][38][39][40][41][42] . Recently, the first transcriptome assemblies of five threatened European freshwater mussels species have been published, including the gill transcriptome of the dolphin freshwater mussel…”

mentioning

confidence: 99%

PacBio Hi-Fi genome assembly of the Iberian dolphin freshwater musselUnio delphinusSpengler, 1793

Gomes‐dos‐Santos

Lopes‐Lima

Machado

et al. 2023

Preprint

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Mussels of order Unionida are a group of strictly freshwater bivalves with nearly 1,000 described species widely dispersed across world freshwater ecosystems. They are highly threatened showing the highest record of extinction events within faunal taxa. Conservation is particularly concerning in species occurring in the Mediterranean biodiversity hotspot that are exposed to multiple anthropogenic threats, possibly acting in synergy. That is the case of the dolphin freshwater mussel Unio delphinus Spengler, 1793, endemic to the western Iberian Peninsula with recently strong population declines. To date, only four genome assemblies are available for the order Unionida and only one European species. We present the first genome assembly of Unio delphinus. We used the PacBio HiFi to generate a highly contiguous genome assembly. The assembly is 2.5 Gb long, possessing 1254 contigs with a contig N50 length of 10 Mbp. This is the most contiguous freshwater mussel genome assembly to date and is an essential resource for investigating the species' biology and evolutionary history that ultimately will help to support conservation strategies.

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Histopathology, antioxidant responses, transcriptome and gene expression analysis in triangle sail mussel Hyriopsis cumingii after bacterial infection

Cited by 26 publications

References 62 publications

The role of outer membrane protein A in OmpA vaccine was revealed by the analysis of alternative splicing and lncRNA after the Edwardsiella anguillarum infected the immunized European eels (Anguilla anguilla)

The role of outer membrane protein A in OmpA vaccine was revealed by the analysis of alternative splicing and lncRNA after the Edwardsiella anguillarum infected the immunized European eels (Anguilla anguilla)

The first use of LC-MS/MS proteomic approach in the brown mussel Perna perna after bacterial challenge: Searching for key proteins on immune response

PacBio Hi-Fi genome assembly of the Iberian dolphin freshwater musselUnio delphinusSpengler, 1793

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