Histopathology combined with transcriptome analyses reveals the mechanism of resistance to Meloidogyne incognita in Cucumis metuliferus

Ye, De-You; Yonghong, Qi; Sufang, Cao; Wei, Bingqiang; Zhang, Huasheng

doi:10.1016/j.jplph.2017.02.002

Cited by 27 publications

(22 citation statements)

References 40 publications

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“…The C. metuliferus accessions assessed in this study were highly resistant (RI < 1%) or resistant (1% ≤ RI ≤ 10%) to most RKN isolates tested. This is in agreement with previous reports by other authors (Fassuliotis, , ; Sigüenza et al ., ; Walters et al ., ; Guan et al ., ; Ye et al ., ). The host suitability of C. metuliferus was not affected by the Mi1.2 (a)virulence of the nematode isolate.…”

Section: Discussionmentioning

confidence: 97%

“…Empty or poorly developed giant cells with multiple vacuoles were observed at 7 and 14 dpi, with giant cells appearing to be collapsed or without cytoplasm. In addition, several genes related to plant defence mechanisms were significantly modified and, in contrast with previous reports (Fassuliotis, ; Walters et al ., ), hypersensitive necrosis was observed (Ye et al ., ). The results of this study are consistent with those previously reported, in which giant cells were multivacuolated or appeared collapsed without cytoplasm.…”

Section: Discussionmentioning

confidence: 97%

“…() in the accession PI 482454 inoculated with M. arenaria , M. hapla , M. incognita or M. javanica . Recent studies (Ye et al ., ) have reported a reduction of the number of M. incognita J2 in roots of the C. metuliferus accession PI 482443 at 7 days post‐inoculation (dpi) compared to at 4 dpi, indicating death or emigration from roots and a delayed development of the nematodes remaining in the roots. Empty or poorly developed giant cells with multiple vacuoles were observed at 7 and 14 dpi, with giant cells appearing to be collapsed or without cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

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Cucumis metuliferus is resistant to root‐knot nematode Mi1.2 gene (a)virulent isolates and a promising melon rootstock

et al. 2018

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Pot experiments were carried out to characterize the response of two Cucumis metuliferus accessions (BGV11135 and BGV10762) against Mi1.2 gene (a)virulent Meloidogyne arenaria, M. incognita and M. javanica isolates and to determine the compatibility and the effect on physicochemical properties of fruit melons. In addition, histopathological studies were conducted. One week after transplanting, plants were inoculated with one J2 cm−3 of sterilized sand (200 cm3 pots) and maintained in a growth chamber at 25 °C for 40 days. The susceptible cucumber cv. Dasher II or melon cv. Paloma were included for comparison. The number of egg masses and number of eggs per plant were assessed, and the reproduction index (RI) was calculated as the percentage of eggs produced on the C. metuliferus accessions compared to those produced on the susceptible cultivars. The compatibility and fruit quality were assessed by grafting three scions, two of Charentais type and one of type piel de sapo, under commercial greenhouse conditions. The resistance level of both C. metuliferus accessions ranged from highly resistant (RI < 1%) to resistant (1% ≤ RI ≤ 10%) irrespective of Meloidogyne isolates. Melon plants grafted onto C. metuliferus accession BGV11135 grew as self‐grafted plants without negatively impacting fruit quality traits. Giant cells induced by Meloidogyne spp. on C. metuliferus were in general poorly developed compared to those on cucumber. Furthermore, necrotic areas surrounding the nematode were observed. Cucumis metuliferus accession BGV11135 could be a promising melon rootstock to manage Meloidogyne spp., irrespective of their Mi1.2 (a)virulence, without melon fruit quality reduction.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Cucumis metuliferus is resistant to root‐knot nematode Mi1.2 gene (a)virulent isolates and a promising melon rootstock

et al. 2018

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“…Additionally, the suppression of ROS detoxifying mechanisms is also essential for the onset of cell death [19]. Feeding cells are also disassociated from surrounding tissue in resistant plants after infection with RKNs [63,64]. As a result, resistant plants produce more ROS, and the accumulation of these components may lead to HR.…”

Section: Discussionmentioning

confidence: 99%

Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars

Chen

Zhao

et al. 2020

IJMS

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In plant immune responses, reactive oxygen species (ROS) act as signaling molecules that activate defense pathways against pathogens, especially following resistance (R) gene-mediated pathogen recognition. Glutathione (GSH), an antioxidant and redox regulator, participates in the removal of hydrogen peroxide (H2O2). However, the mechanism of GSH-mediated H2O2 generation in soybeans (Glycine max (L.) Merr.) that are resistant to the soybean cyst nematode (SCN; Heterodera glycines Ichinohe) remains unclear. To elucidate this underlying relationship, the feeding of race 3 of H. glycines with resistant cultivars, Peking and PI88788, was compared with that on a susceptible soybean cultivar, Williams 82. After 5, 10, and 15 days of SCN infection, we quantified γ-glutamylcysteine (γ-EC) and (homo)glutathione ((h)GSH), and a gene expression analysis showed that GSH metabolism in resistant cultivars differed from that in susceptible soybean roots. ROS accumulation was examined both in resistant and susceptible roots upon SCN infection. The time of intense ROS generation was related to the differences of resistance mechanisms in Peking and PI88788. ROS accumulation that was caused by the (h)GSH depletion-arrested nematode development in susceptible Williams 82. These results suggest that (h)GSH metabolism in resistant soybeans plays a key role in the regulation of ROS-generated signals, leading to resistance against nematodes.

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“…In the case of C. metuliferus , the resistance response to RKN is associated with hindrance of larval development, delayed development from second‐stage juveniles (J2) to adults, increased maleness of J2, migration of J2 from the root, differential expression of several genes related to plant defence mechanisms, and the appearance of necrotic areas surrounding the nematode . Rotation sequences including solanaceous and cucurbits species in protected cultivation are very common because these crops represent the main source of income for many growers .…”

Section: Introductionmentioning

confidence: 99%

Cucumis metuliferus reduces Meloidogyne incognita virulence against the Mi1.2 resistance gene in a tomato–melon rotation sequence

Expósito

García

Giné

et al. 2019

Pest Management Science

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BACKGROUND: Susceptible tomato cv. Durinta, ungrafted or grafted onto cv. Aligator resistant rootstock, both followed by the susceptible melon cv. Paloma, ungrafted or grafted onto Cucumis metuliferus BGV11135, and in the reverse order, were cultivated from 2015 to 2017 in the same plots in a plastic greenhouse, infested or not with Meloidogyne incognita. For each crop, soil nematode densities, galling index, number of eggs per plant and crop yield were determined. Virulence selection was evaluated in pot experiments. RESULTS:In the tomato-melon rotation, nematode densities increased progressively for the grafted tomato, being higher than for ungrafted plants at the end of the study; this was not the case in the melon-tomato rotation. Grafted crops yielded more than ungrafted crops in the infested plots. Virulence against the Mi1.2 gene was detected, but not against C. metuliferus. Reproduction of M. incognita on the resistant tomato was ∼ 120% that on the susceptible cultivar after the first grafted tomato crop, but this decreased to just 25% at the end of the experiment. CONCLUSION: Alternating different resistant plant species suppresses nematode population growth rate and yield losses.Although this strategy does not prevent virulence selection, the level was reduced.

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Histopathology combined with transcriptome analyses reveals the mechanism of resistance to Meloidogyne incognita in Cucumis metuliferus

Cited by 27 publications

References 40 publications

Cucumis metuliferus is resistant to root‐knot nematode Mi1.2 gene (a)virulent isolates and a promising melon rootstock

Cucumis metuliferus is resistant to root‐knot nematode Mi1.2 gene (a)virulent isolates and a promising melon rootstock

Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars

Cucumis metuliferus reduces Meloidogyne incognita virulence against the Mi1.2 resistance gene in a tomato–melon rotation sequence

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