Historical Perspective — Development of nif Genetics and Regulation in Klebsiella pneumoniae

Dixon, Ray

doi:10.1007/1-4020-2179-8_1

Cited by 1 publication

(1 citation statement)

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“…This nitrogen fixation process requires an enzyme called nitrogenase (Dixon and Kahn 2004). Most regulatory studies on nitrogen fixation are based on the Gram‐negative model using Klebsiella pneumoniae and Azotobacter vinelandii (Dixon 2004, Kennedy and Bishop 2004). Clostridium pasteurianum was one of the few Gram‐positive diazotrophs used as a model.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of nitrogen fixation in Paenibacillus durus during growth in nitrogen‐enriched medium

Halim

Choo

Ghazali

et al. 2021

Lett Appl Microbiol

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Paenibacillus durus strain ATCC 35681T is a Gram‐positive diazotroph that displayed capability of fixing nitrogen even in the presence of nitrate or ammonium. However, the nitrogen fixation activity was detected only at day 1 of growth when cultured in liquid nitrogen‐enriched medium. The transcripts of all the nifH homologues were present throughout the 9‐day study. When grown in nitrogen‐depleted medium, nitrogenase activities occurred from day 1 until day 6 and the nifH transcripts were also present during the course of the study albeit at different levels. In both studies, the absence of nitrogen fixation activity regardless of the presence of the nifH transcripts raised the possibility of a post‐transcriptional or post‐translational regulation of the system. A putative SigA box sequence was found upstream of the transcription start site of nifB1, the first gene in the major nitrogen fixation cluster. The upstream region of nifB2 showed a promoter recognizable by SigE, a sigma factor normally involved in sporulation.

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Section: Introductionmentioning

confidence: 99%