History, Evolution and Current State of Ovarian Tissue Auto-Transplantation with Cryopreserved Tissue: a Successful Translational Research Journey from 1999 to 2020

Marin, Loris; Bedoschi, Giuliano; Kojima, Tai; Oktay, Kutluk

doi:10.1007/s43032-019-00066-9

Cited by 56 publications

(29 citation statements)

References 51 publications

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“…More and more data and evidence support the use of ovarian tissue cryopreservation for fertility protection, and we are gaining increasing knowledge about how this technique could be further improved (Forman 2018;Marin et al, 2020;Dolmans et al, 2013;Ruan et al, 2019;Jin et al, 2019).…”

Section: Low-temperature Transportmentioning

confidence: 99%

Long-time low-temperature transportation of human ovarian tissue before cryopreservation

Cheng

Ruan

Zhou

et al. 2021

Reproductive BioMedicine Online

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Research question: Can the low-temperature transport time of removed human ovarian tissue be prolonged until cryopreservation? Design: Fresh ovarian cortex from nine premenopausal patients was either slow-programmed frozen immediately or stored at 4℃ for 24h or 48h before slow-freezing. The fresh and frozen-thawed biopsies were evaluated by follicle counting via calcein-staining, histologic analyses via HE, and apoptosis via TUNEL. The fresh cortex was assessed by ROS and TAC assay for oxidative stress 2 2 detection. The frozen-thawed cortex biopsies were also evaluated by qPCR for mRNA expression of BCL-2, BAX, TNFa, HIF-1a, BMP15, and GDF9, western-blot for detection of BCL-2, BMP15, GDF9, and CASPASE3. The frozen-thawed cortex was cultured in-vitro for four-days, AMH and glucose were assessed in the supernatant, and ROS and TAC assay detected cortex's oxidative stress.Results: In the fresh cortex, no significant differences between the three groups. In the frozen-thawed cortex, no significant differences between the three groups regarding follicle viability, TUNEL, mRNA expression of TNFa, HIF-1a, and BMP15. GDF9 mRNA and BAX/BCL-2 were lower and higher at 48h than at 0h, respectively. However, the protein expression of BCL-2, CASPASE3, GDF9, and BMP15 were not different. In the cultured cortex, ROS, TAC, and glucose uptake were not different in the three groups. Conclusion:Ovarian tissue transportation was validated for up to 24h in the practical procedure. This study showed that 4-8℃ transportation for 24h or 48h does not seem to damage the ovarian tissue. However, ovarian tissue transportation above 48h still needs to be further studied for conclusions to be made.

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Section: Low-temperature Transportmentioning

confidence: 99%

Long-time low-temperature transportation of human ovarian tissue before cryopreservation

Cheng

Ruan

Zhou

et al. 2021

Reproductive BioMedicine Online

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“…I Norge er dette et tilbud til kvinner <35 år med minst 50 % risiko for infertilitet som følge av kreftbehandling (180;181;183). Foreløpig er det et fåtall fødsler etter denne metoden i Norge, men nyere forskning rapporterer 25-30 % «live birth rates» etter retransplantasjon av ovarialvev i bekkenet/kontralaterale ovar (184). Det lave antall fødsler i Norge er vesentlig betinget i at få kvinner kommer tilbake med ønske om retransplantasjon.…”

Section: Kvinnerunclassified

Sykepleiere må kunne snakke om seksuell helse etter gynekologisk kreft

Hoel¹,

Leite²,

Grønvik³

2021

Sykepleien

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“…Since then, the number has risen exponentially, more than doubling between 2015 and 2017. 13 As of 2017, over 130 live-births have been reported worldwide, 14 with the actual number likely much higher due to underreporting (Fig. 2).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ovarian Tissue for Pediatric Fertility

Hinkle

Orwig

Valli-Pulaski

et al. 2021

Biopreservation and Biobanking

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Intensive treatments necessary to treat some childhood malignancies and other conditions, as well as certain anatomic variations, may lead to infertility in adulthood. Until recently, no fertility preservation options for prepubertal females were available. However, ovarian tissue cryopreservation has emerged as a safe and effective option for these children. In the next several years, it is likely that more pediatric patients, their families, and medical teams will pursue an ovarian cryopreservation protocol at their institutions. Patient selection, consenting, and laparoscopic oophorectomy can be done at many centers. Then, the ovarian tissue is initially processed and transported to a specialized center for processing for cryopreservation. The cryopreservation techniques are best performed at appropriately certified centers processing high volumes of reproductive cells/tissues with expert personnel and specialized equipment. This article aims to provide an overview for pediatric biobank professionals who may be called to participate in this or similar protocols.

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History, Evolution and Current State of Ovarian Tissue Auto-Transplantation with Cryopreserved Tissue: a Successful Translational Research Journey from 1999 to 2020

Cited by 56 publications

References 51 publications

Long-time low-temperature transportation of human ovarian tissue before cryopreservation

Long-time low-temperature transportation of human ovarian tissue before cryopreservation

Sykepleiere må kunne snakke om seksuell helse etter gynekologisk kreft

Cryopreservation of Ovarian Tissue for Pediatric Fertility

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