1996
DOI: 10.1016/0165-2478(96)02579-5
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HIV-1 epitopes exposed by hybrid hepatitis B core particles affect proliferation of peripheral blood mononuclear cells from HIV-1 positive donors

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Cited by 6 publications
(3 citation statements)
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“…70 This same epitope was more successful in neutralizing antibody production when presented on the influenza A vaccine platform, thus the molecular context seems to be important when considering the ability to elicit protective responses. However, correct processing of particles or T‐cell recognition of HIV epitopes on particles is implied on HBV because both peptides and particles with gp41 epitope elicited similar responses 71…”
Section: Using Vlps As Platforms For Displaying and Inducing Immune Rmentioning
confidence: 99%
“…70 This same epitope was more successful in neutralizing antibody production when presented on the influenza A vaccine platform, thus the molecular context seems to be important when considering the ability to elicit protective responses. However, correct processing of particles or T‐cell recognition of HIV epitopes on particles is implied on HBV because both peptides and particles with gp41 epitope elicited similar responses 71…”
Section: Using Vlps As Platforms For Displaying and Inducing Immune Rmentioning
confidence: 99%
“…The N-terminus of HBc molecule was used also as a target for insertion of relatively short epitopes from HBV preS [102][103][104]; HIV-1 gp120 and p24 [105], gp41, p34 Pol, and p17 Gag [106,107]; and from human cytomegalovirus gp58 [108]. The latter could not be purified or characterized immunologically, although it formed VLPs.…”
Section: N-terminal Insertionsmentioning
confidence: 99%
“…Peripheral mononuclear cells (PBMCs) were isolated by Ficoll Paque gradient centrifugation of blood which was collected in heparinized Vacutainer tubes. PBMCs were subjected to in vitro stimulation with core-derived synthetic peptides ( Table 1 ) using the procedure described by us earlier [ 38 ]. In brief, T-cell proliferation assay was performed in triplicate with RPMI containing HCV core-derived peptides, all at 1 mcg/well; phytohemagglutinin (PHA; 10 mcg/well) was used as positive and RPMI alone and control peptide representing aa 605–613 of gp41 of HIV-1 were used as negative controls.…”
Section: Methodsmentioning
confidence: 99%