HIV serology using piezoelectric immunosensors

Aberl, Franz; Wolf, Hans; Kößlinger, Conrad; Drost, S.; Woias, Peter; Koch, Sabine

doi:10.1016/0925-4005(94)87093-4

Cited by 70 publications

(29 citation statements)

References 2 publications

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“…The applicability of piezoimmunosensors in various fields is now well established A B [98]. The range extends from applications in clinical analysis [93,95,[99][100][101][102][103][104][105][106][107][108] through the determination of bacteria [94,[109][110][111][112][113][114] in the food industry to environmental analysis for the detection of organic compounds by using an antigen-antibody reaction [101,[115][116][117][118][119]. Since the invention of phage libraries, immunosensing systems based on the QCM have been successfully applied as devices for the screening of phage libraries and determination of antibody affinity [120][121][122].…”

Section: Piezoimmunosensorsmentioning

confidence: 99%

Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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The quartz crystal microbalance (QCM) was first introduced as a mass sensor in gas phase and in vacuum. Since oscillator circuits capable of exciting shear vibrations of quartz resonators under liquid load have been developed, the QCM became accepted as a new powerful technique to monitor adsorption processes at solid/liquid interfaces in chemical and biological research rendering the method an attractive low‐cost alternative for bioanalytic applications. In the last decade, adsorption of biomolecules on functionalized surfaces turned out to be one of the paramount applications of piezoelectric transducers comprising the interaction of DNA and RNA with complementary strands, specific recognition of protein‐ligands by immobilized receptors, the detection of virus capsids, bacteria, mammalian cells and last but not least the development of complete immunosensors. Piezoelectric transducers allow a label‐free detection of molecules; they are more than mere mass sensors since the sensor response is also influenced by interfacial phenomena, viscoelastic properties of the adhered biomaterial, surface charges of adsorbed molecules and surface roughness. These new insights have recently been used to investigate the adhesion of cells, liposomes and proteins onto surfaces allowing to determine morphological changes of cells as a response to pharmacological substances and changes in the water content of biopolymers in situ. However, future will show whether the quartz crystal microbalance will assert itself against established label‐free sensor devices like surface plasmon resonance spectroscopy and interferometry.

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Section: Piezoimmunosensorsmentioning

confidence: 99%

Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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“…Furthermore, piezoelectric sensors are inexpensive, easy-to-use, and feature rapid response; hence, they may allow for wide screenings and the development of effective preventive strategies for a broad range of diseases. Such sensors are used to determine the concentration of immunoglobulin IgM and C-reactive protein [112]; human cells such as T-lymphocytes [113], erythrocytes [114], herbicides in drinking water [115]; bacteria such as E.coli [116], Stalphylococcus aureus [117]; drugs (methamphetamine) in human urine [118]; and viruses such as human herpes [119], hepatitis [120], African swine fever [121], and HIV [122]. In particular, piezoelectric sensors facilitated the detection of antibodies that are specific against HIV within a few minutes in human serum sample.…”

Section: ) Acoustic Waves Piezoelectric Transducers and Acoustic Sementioning

confidence: 99%

Emerging biomedical sensing technologies and their applications

2003

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Abstract-Recent progress in biomedical sensing technologies has resulted in the development of several novel sensor products and new applications. Modern biomedical sensors developed with advanced microfabrication and signal processing techniques are becoming inexpensive, accurate, and reliable. A broad range of sensing mechanisms has significantly increased the number of possible target measurands that can be detected. The miniaturization of classical "bulky" measurement techniques has led to the realization of complex analytical systems, including such sensors as the BioChemLab-on-a-Chip. This rapid progress in miniature devices and instrumentation development will significantly impact the practice of medical care as well as future advances in the biomedical industry. Currently, electrochemical, optical, and acoustic wave sensing technologies have emerged as some of the most promising biomedical sensor technologies. In this paper, important features of these technologies, along with new developments and some of the applications, are presented.

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“…The resulting signals were converted in the computer program Origin and presented as a frequency/time profile. For the measurements gold-coated 20 MHz AT-cut quartz crystals (XA 1600) 8 mm in diameter and 84 m in thickness were used with gold electrodes (100 nm layers with a diameter of 4 mm) connecting to an oscillator circuit (24,25), which regulates its frequency at the minimum impedance of the quartz crystal. Twenty-megahertz quartz crystals were used due to an increased sensitivity of these sensors in comparison to the commonly used 5-MHz piezoelectric plates (26).…”

Section: Methodsmentioning

confidence: 99%