HIWI2 induces G2/M cell cycle arrest and apoptosis in human fibrosarcoma via the ROS/DNA damage/p53 axis

Das, Basudeb; Sahoo, Swapnil; Mallick, Bibekanand

doi:10.1016/j.lfs.2022.120353

Cited by 18 publications

(11 citation statements)

References 49 publications

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“…Even several studies also reported that pro-apoptotic protein also activated without p53 through extrinsic insults that directly induced apoptosis [ 42 ]. The Bax protein is localized in the outer mitochondrial membranes and various reports imply that it induced apoptosis by promoting the release of cytochrome c from mitochondria to activate caspase-9 [ 43 ]. We found that AGA extract increases the apoptosis in all colon cancer cells by upregulating the expression levels of pro-apoptotic proteins Bax and caspase-9 while downregulating the p53 expression in SW620 and SW480 colon cancer cell line to mediate p53-independent apoptosis.…”

Section: Discussionmentioning

confidence: 99%

AGA induces sub-G1 cell cycle arrest and apoptosis in human colon cancer cells through p53-independent/p53-dependent pathway

et al. 2023

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Background Despite the advancement in chemotherapeutic drugs for colon cancer treatment, it is still a life-threatening disease worldwide due to drug resistance. Therefore, an urgently needed to develop novel drugs for colon cancer therapies. AGA is a combination of traditional Chinese medicine Antler’s extract (A), Ganoderma lucidum (G), and Antrodia camphorata (A); it contains a lot of biomolecules like polysaccharides, fatty acids, and triterpenoids that are known to exerting anti-oxidative, anti-inflammatory, anti-microbial and anti-tumor activities in oral cancer. In this study, we investigate AGA anti-proliferative, anti-metastatic and apoptotic activity to explore its anti-cancer activity against colon cancer cells and its underlying mechanism. Method Here, in-vitro studies were performed to determine the antiproliferative activity of AGA through MTT and colony formation assays. Wound healing and transwell migration assay were used to evaluate the metastasis. Flow cytometry and protein expression were used to investigate the involved molecular mechanism by evaluating the cell cycle and apoptosis. The in-vivo anti-cancerous activity of AGA was assessed by xenograft mice model of colon cancer cells. Results We found that AGA significantly inhibited the proliferative capacity and metastasis of colon cancer cells in-vitro. In addition, AGA induced cell cycle arrest in the sub-G1 phase through upregulating p21 and downregulating CDK2, CDK6 in SW620, and CDK4 in SW480 and HT29, respectively. Annexin-v assay indicated that colon cancer cells had entered early and late apoptosis after treatment with AGA. Furthermore, a mechanistic protein expressions study revealed that AGA in p53-dependent and independent regulated the apoptosis of colon cancer by downregulating the p53 protein expression in SW620 and SW480 cells but upregulating in a dose-dependent manner in HT29 cells and increasing the expression of Bax and caspase-9 to inhibit the colon cancer cells. In vivo study, we found that AGA significantly reduced the xenograft tumor growth in NOD/SCID mice with no adverse effect on the kidney and liver. Conclusion Collectively, AGA has the potential to inhibit colon cancer through inhibiting proliferation, migration, and cell cycle kinase by upregulating p21 protein expression and promoting the apoptotic protein in a p53-dependent and independent manner.

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Section: Discussionmentioning

confidence: 99%

AGA induces sub-G1 cell cycle arrest and apoptosis in human colon cancer cells through p53-independent/p53-dependent pathway

et al. 2023

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“…In the present study, the presence of Zn 2+ ions on the surface of the composite coating is evident from the obtained XPS data (Figure ), which further confirms the possibility for bacterial death through this mechanism. Finally, the ROS generation capability of the rGO–ZnO nanocomposite can also induce bacterial cell death by damaging cellular components, such as proteins, enzymes, lipids, and DNA. , The photocatalytic activity (Figure B) and effective electron–hole charge separation achieved in the rGO–ZnO nanocomposite by adjusting the composition can result in the generation of ROS when it comes in contact with the medium, in the presence of light. , Thus, generated ROS species such as superoxide anion (O 2 – ), superoxide radical ( • O 2 – ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (HO • ) can interact with the bacterial cells and lead to bacterial death . Fluorometric assay was employed to detect the presence of reactive oxygen species on the coating surface to confirm the mechanism of bacterial death due to ROS .…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the obtained FESEM results support that the existence of needle-like ZnO, spherical ZnO nanoparticles, and rGO nanosheets causes physical damage to bacterial cells, and the ROS generation on the surface also leads to the damage of DNA, cell wall, and cell-proteins of the bacteria. 32,33 3.5.2.2. EDAX.…”

Section: Evaluation Of Bacterial Adhesion and The Effect Of Biofilm F...mentioning

confidence: 99%

Multifunctional rGO–ZnO Composite Coatings with Enhanced Mechanical, Anticorrosion, and Antibacterial Characteristics for Practical Applications

Geethanjali,

Elias,

Bijimol

et al. 2023

ACS Appl. Eng. Mater.

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This paper reports the development of multifunctional rGO–ZnO composite (RGZ) coatings with enhanced mechanical, anticorrosion, and antibacterial characteristics for practical marine applications. The structure and composition of the RGZ composite coatings are tuned to achieve distinct and compact intermetallic alloy layers, leading to improved hardness, wear resistance, friction resistance, electrochemical stability, and antibacterial activity. 0.3 wt %-RGZ composite coating with the highest R ct value (367.8 Ω cm2) exhibits the least corrosion rate (0.75 mmpy) as compared to the other compositions of the developed coatings. All the RGZ composite coatings exhibit enhanced antibacterial activity in different bacterial suspensions selected for the present study (Escherichia coli, Bacillus subtilis, and seawater consortium), and the 0.3 wt %-RGZ composite coating is demonstrated to be suitable for marine applications with the lowest bacterial survivability (20.3%) in the seawater consortium. The electrochemical stability and anticorrosion performance of the 0.3 wt %-RGZ composite coating even after long-term exposure (28 days) to seawater consortium confirmed from the largest R ct value (1154.7 Ω cm2) and the least i corr value (223.4 μA/cm2) indicate its suitability for practical application with excellent reusability. The enhanced antibacterial activity of the 0.3 wt %-RGZ composite coating as compared to other compositions of RGZ coatings and bare-Zn coating is attributed to its three-level bacterial destruction capability by inducing damage to the bacterial cell wall through physical damage, penetration of Zn2+ ions, and the activity of the photogenerated reactive oxygen species (ROS). The enhanced antibacterial activity and excellent electrochemical stability with attractive anticorrosion performance of 0.3 wt %-RGZ composite coating even after long-term exposure (28 days) in different bacterial suspensions, especially in seawater consortium, confirm its suitability for practical applications.

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“…In brain at 28 th day of exposure with As 2 O 3 , there is increase expression of p53 was seen. Individually delayed division of cells as well as decreases the time taken for G1-cell cycle arrested and damage of DNA/mitochondrial membrane have been generally stated that elevated expressions of p53 in exposed cells to As 2 O 3 as an agents for genotoxicity [Figure 3] [16].…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization and expression profiling of arsenic mediated stress-responsive genes in Dawkinsia tambraparniei (Silas, 1954)

Sakthivel¹,

Dhanapal²,

Munisamy³

et al. 2022

J App Biol Biotech

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HIWI2 induces G2/M cell cycle arrest and apoptosis in human fibrosarcoma via the ROS/DNA damage/p53 axis

Cited by 18 publications

References 49 publications

AGA induces sub-G1 cell cycle arrest and apoptosis in human colon cancer cells through p53-independent/p53-dependent pathway

AGA induces sub-G1 cell cycle arrest and apoptosis in human colon cancer cells through p53-independent/p53-dependent pathway

Multifunctional rGO–ZnO Composite Coatings with Enhanced Mechanical, Anticorrosion, and Antibacterial Characteristics for Practical Applications

Molecular characterization and expression profiling of arsenic mediated stress-responsive genes in Dawkinsia tambraparniei (Silas, 1954)

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