HLA-A, -B, -DRB1, -DQA1, and -DQB1 polymorphism in thais

Chandanayingyong, Dasnayanee; Stephens, Henry A.F.; Klaythong, R.; Sirikong, Monchan; Udee, Sudchai; Longta, Panimon; Chantangpol, Rapeepun; Bejrachandra, Sasitorn; Rungruang, Ekaraj

doi:10.1016/s0198-8859(96)00284-4

Cited by 72 publications

(95 citation statements)

References 16 publications

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“…HLA-B*07 and other alleles of the B*07-like "supertype" (HLA-B35, -B51, -B53, -B54, -B55, -B56, -B67, and -B78) are common in Caucasoid, African, and northeast Asian populations (49) but are less well represented in mainland southeast Asians, including the Thais (24). These alleles share a preference for binding peptides characterized by a proline at position 2 and an aromatic or hydrophobic residue at the C terminus and also have similar amino acids forming the B peptide-binding pocket (49).…”

Section: Discussionmentioning

confidence: 99%

“…Serologic HLA class I typing was performed on fresh blood obtained 6 mo or more after the acute infection at the Department of Transfusion Medicine, Siriraj Hospital (Bangkok, Thailand), using standard techniques (24). The presence of HLA-B*07-related alleles was confirmed by direct and semiautomated sequencing of HLA-B exons 2 and 3 in genomic DNA, using the primers and protocols developed for the 12th International HLA Workshop (25).…”

Section: Study Design and Blood Samplesmentioning

confidence: 99%

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T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

Zivna

Green

Vaughn

et al. 2002

The Journal of Immunology

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128

117

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Dengue hemorrhagic fever (DHF), the severe manifestation of dengue virus (DV) infection characterized by plasma leakage, is more common in secondary DV infections in previously infected individuals and is associated with high levels of immune activation. To determine the Ag specificity of this immune response, we studied the response to an HLA-B*07-restricted T cell epitope, residues 221–232 of the DV NS3 protein, in 10 HLA-B*07+ Thai children who were studied during and after acute DV infections. Peptide-specific T cells were detected in 9 of 10 subjects. The frequency of peptide-specific T cells was higher in subjects who had experienced DHF than in those who had experienced DF. We also detected peptide-specific T cells in PBMC obtained at the time of the acute DV infection in 2 of 5 subjects. These data suggest that the NS3 (221–232) epitope is an important target of CD8+ T cells in secondary DV infection and that the activation and expansion of DV-specific T cells is greater in subjects with DHF than in those with dengue fever. These findings support the hypothesis that activation of DV-specific CD8+ T cells plays an important role in the pathogenesis of DHF.

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Section: Discussionmentioning

confidence: 99%

Section: Study Design and Blood Samplesmentioning

confidence: 99%

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

Zivna

Green

Vaughn

et al. 2002

The Journal of Immunology

Self Cite

128

117

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show abstract

“…For this reason, we used a dual approach to define epitopes, studying samples from both convalescent children and healthy immune adults. To study the role of T cell responses in the pathology of Dengue, we were particularly interested to define epitopes restricted by common HLA alleles found in Southeast Asia, namely for MHC class I, A*02 (20%), A*11 (30%), A*24 (20%), and A*33 (15%) (17). Dengue is a negative-strand RNA virus, which encodes a polyprotein of ϳ3300 aas, which is cleaved into 10 polypeptides by both host and virally encoded proteases.…”

Section: Definition Of a Dengue Cd8mentioning

confidence: 99%

T Cell Responses in Dengue Hemorrhagic Fever: Are Cross-Reactive T Cells Suboptimal?

Mongkolsapaya

Duangchinda

Dejnirattisai

et al. 2006

The Journal of Immunology

234

221

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Dengue virus infection poses a growing public health and economic burden in a number of tropical and subtropical countries. Dengue circulates as a number of quasispecies, which can be divided by serology into four groups or serotypes. An interesting feature of Dengue, recognized over five decades ago, is that most severe cases that show hemorrhagic fever are not suffering from a primary infection. Instead, they are reinfected with a virus of different serotype. This observation poses considerable problems in vaccine design, and it is therefore imperative to gain a full understanding of the mechanisms underlying this immunological enhancement of disease. In this study, we examined a T cell epitope restricted by HLA-A*24, a major MHC class I allele, in Southeast Asia in a cohort of children admitted to a hospital with acute Dengue infection. The cytokine profiles and the degranulation capacity of T cells generated to this epitope are defined and compared across different viral serotypes. Cross-reactive Dengue-specific T cells seem to show suboptimal degranulation but high cytokine production, which may contribute to the development of the vascular leak characteristic of Dengue hemorrhagic fever.

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“…HLA Class I serotyping was performed on peripheral blood lymphocytes within 24 hr of collection, using a standard two-stage microlymphocytotoxicity test for HLA-A, B, and C, as previously described. 22 HLA class II molecular typing of the DRB1, DQA1, DQB1, and DPB1 gene loci was performed on genomic DNA, using the 11th and 12th International Histocompatibility Workshop primers, sequence-specific oligonucleotide probes and protocols as previously described. 22,23 Statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

“…22 HLA class II molecular typing of the DRB1, DQA1, DQB1, and DPB1 gene loci was performed on genomic DNA, using the 11th and 12th International Histocompatibility Workshop primers, sequence-specific oligonucleotide probes and protocols as previously described. 22,23 Statistical analysis. Pearson correlation was used to compare the expression of all TCR V␤ gene families in serial analyses of the same PBMC samples.…”

Section: Methodsmentioning

confidence: 99%

T cell receptor Vbeta gene usage in Thai children with dengue virus infection.

Gagnon

Leporati²,

Green³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. T lymphocyte activation during dengue is thought to contribute to the pathogenesis of dengue hemorrhagic fever (DHF). We examined the T cell receptor V␤ gene usage by a reverse transcriptase-polymerase chain reaction assay during infection and after recovery in 13 children with DHF and 13 children with dengue fever (DF). There was no deletion of specific V␤ gene families. We detected significant expansions in usage of single V␤ families in six subjects with DHF and three subjects with DF over the course of infection, but these did not show an association with clinical diagnosis, viral serotype, or HLA alleles. Differences in V␤ gene usage between subjects with DHF and subjects with DF were of borderline significance. These data suggest that the differences in T cell activation in DHF and DF are quantitative rather than qualitative and that T cells are activated by conventional antigen(s) and not a viral superantigen.

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HLA-A, -B, -DRB1, -DQA1, and -DQB1 polymorphism in thais

Cited by 72 publications

References 16 publications

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

T Cell Responses in Dengue Hemorrhagic Fever: Are Cross-Reactive T Cells Suboptimal?

T cell receptor Vbeta gene usage in Thai children with dengue virus infection.

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