HLA-DP typing by amplified fragment length polymorphisms (AFLPs)

Dekker, James; Easteal, Simon

doi:10.1007/bf01787330

Cited by 35 publications

(11 citation statements)

References 10 publications

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“…DNA was extracted from washed buffy coat cell pellets using a Genepure Nucleic Acid Purification System -Model 341 (Applied Biosystems Inc., Warrington, UK). HLA-DPBl typing of 19 recognized alleles [16] was performed by restriction enzyme analysis of the second exon of DPBl amplified by PCR as described [17]. HLA-DRBl typing of 12 alleles was performed using sequence-specific oligonucleotide probing of PCR products as described previously [18].…”

Section: Methodsmentioning

confidence: 99%

Aspirin-induced asthma and HLA-DRB1 and HLA-DPB1 genotypes

DEKKER¹,

NIZANKOWSKA²,

SCHMITZ-SCHUMANN³

et al. 1997

Clin Exp Allergy

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Section: Methodsmentioning

confidence: 99%

Aspirin-induced asthma and HLA-DRB1 and HLA-DPB1 genotypes

DEKKER¹,

NIZANKOWSKA²,

SCHMITZ-SCHUMANN³

et al. 1997

Clin Exp Allergy

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“…To distinguish between Cw*0102 and Cw*0103, two primer pairs, 5C01: 5ø-CCC ACT CCA TGA AGT ATT TCT T-3ø for a forward primer and 3C102: 5ø-CGT CGT AGG CGT ACT GGT C-3ø or 3C103: 5ø-CGT CGT AGG CGA ACT GGT T-3ø for a reverse primer were 523 Tissue Antigens 2000: 56: 522-529 designed, giving a 593-bp PCR fragment from Cw*0102 or Cw*0103, respectively. HLA-DRB1, DQB1 and DPB1 allele typing was mainly performed by PCR-RFLP as previously described (15)(16)(17)(18)(19)(20)(21). As a supplementary method, PCR-SSP was also used (22-24).…”

Section: Hla Class I and Class Ii Allele Typingmentioning

confidence: 99%

Allele frequencies and haplotypic associations defined by allelic DNA typing at HLA class I and class II loci in the Japanese population

et al. 2000

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HLA class I and class II allelic genotypes were determined in 371 unrelated individuals and 309 members of 81 families inhabiting the central Japan area. A total of 20 HLA-A alleles, 16 HLA-Cw alleles, 38 HLA-B alleles, 27 HLA-DRB1 alleles, 15 HLA-DQB1 alleles and 12 HLA-DPB1 alleles were detected. By the two-, three-, four-, five- and six-locus allelic association analyses extracted from the HLA-A to -DPB1 locus, 26 HLA-Cw-B haplotypes, 25 HLA-DRB1-DQB1 haplotypes, 42 HLA-Cw-B-DRB1 haplotypes, 37 HLA-Cw-B-DRB1-DQB1 haplotypes, 29 HLA-A-Cw-B-DRB1-DQB1 haplotypes and 21 HLA-A-Cw-B-DRB1-DQB1-DPB1 haplotypes with the frequencies of higher than 0.005 were recognized. Among 19 HLA-B alleles with the high allele frequencies (above 0.007), 9 HLA-B alleles, B*0702, B*1301, B*3701, B*3901, B*4006, B*4403, B*5201, B*5901 and B*6701 were found to be tightly associated with single HLA-Cw alleles. Most of HLA-DRB1 alleles showed strong associations with single HLA-DQB1 alleles, but DRB1*0802 and DRB1*1401 were associated with two different DQB1 alleles. Extended haplotypes carrying infrequent class I alleles with the allele frequencies of lower than 0.007 were defined by family studies. Gene frequencies and haplotypic associations within the entire HLA classical loci elucidated at the high resolution (four-digital) allelic level will provide useful information on anthropology, marrow donor registry, legal medicine and disease-association studies.

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“…and HLA DRB 1*12) and for HLA-DRB 1*06 (HLA-DRB 1*13 and HLA-DRB 1*14). The typing of 19 recognized HLA DPBl alleles (HLA DPB1*1-19) was performed by amplified fragment length polymorphism (AFLP) analysis, using the following restriction endonucleases Bst Ul, Dde I, Eco NI, Eok I, Mnl I, Rsa I and Sau 96 I, [18],…”

Section: Hla Typingmentioning

confidence: 99%

Sensitivity to bee and wasp venoms: association with specific IgE responses to the bee and wasp venom and HLA DRB1 and DPB1

Faux

Moffatt

Lalvani

et al. 1997

Clin Experimental Allergy

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Patients monosensitive to bee venom had a significantly greater prevalence of DRB1*07 alleles than the non-venom, control population suggesting that IgE responses in these patients may, in part be controlled by immune response HLA class II genes. These results are also suggestive of wasp-sensitive patients having a higher prevalence of DRB1*11 and a lower prevalence of DRB1*04 than the control population.

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HLA-DP typing by amplified fragment length polymorphisms (AFLPs)

Cited by 35 publications

References 10 publications

Aspirin-induced asthma and HLA-DRB1 and HLA-DPB1 genotypes

Aspirin-induced asthma and HLA-DRB1 and HLA-DPB1 genotypes

Allele frequencies and haplotypic associations defined by allelic DNA typing at HLA class I and class II loci in the Japanese population

Sensitivity to bee and wasp venoms: association with specific IgE responses to the bee and wasp venom and HLA DRB1 and DPB1

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