HMGB proteins and transcriptional regulation

Ueda, Tetsuya; Yoshida, Michiteru

doi:10.1016/j.bbagrm.2009.11.005

Cited by 125 publications

(103 citation statements)

References 67 publications

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“…It has been well-established that HMGB proteins can regulate gene-specific transcription through interaction with transcription factors, as well as remodeling chromatin structure (Laurent et al, 2010;Ueda and Yoshida, 2010). Transcription factors identified as the partner of HMGB protein have been well reviewed (Agresti and Bianchi, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…It has been well documented that HMG proteins not only act as dynamic modulators of chromatin architecture but also especially influence DNA replication, recombination, repair and transcriptional regulation (Agresti and Bianchi, 2003;Bianchi and Agresti, 2005;Ueda and Yoshida, 2010). Multiple transcription factors have been verified to interact with HMG proteins at specific gene locus during the process of transcription (Ueda and Yoshida, 2010).…”

Section: Introductionmentioning

confidence: 99%

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HMGB2 regulates satellite-cell-mediated skeletal muscle regeneration through IGF2BP2

Zhou

Huang

et al. 2016

Journal of Cell Science

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Although the mechanism underlying modulation of transcription factors in myogenesis has been well elucidated, the function of the transcription cofactors involved in this process remains poorly understood. Here, we identified HMGB2 as an essential nuclear transcriptional co-regulator in myogenesis. HMGB2 was highly expressed in undifferentiated myoblasts and regenerating muscle. Knockdown of HMGB2 inhibited myoblast proliferation and stimulated its differentiation. HMGB2 depletion downregulated Myf5 and cyclin A2 at the protein but not mRNA level. In contrast, overexpression of HMGB2 promoted Myf5 and cyclin A2 protein upregulation. Furthermore, we found that the RNA-binding protein IGF2BP2 is a downstream target of HMGB2, as previously shown for HMGA2. IGF2BP2 binds to mRNAs of Myf5 or cyclin A2, resulting in translation enhancement or mRNA stabilization, respectively. Notably, overexpression of IGF2BP2 could partially rescue protein levels of Myf5 and cyclin A2, in response to HMGB2 decrease. Moreover, depletion of HMGB2 in vivo severely attenuated muscle repair; this was due to a decrease in satellite cells. Taken together, these results highlight the previously undiscovered and crucial role of the HMGB2-IGF2BP2 axis in myogenesis and muscle regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HMGB2 regulates satellite-cell-mediated skeletal muscle regeneration through IGF2BP2

Zhou

Huang

et al. 2016

Journal of Cell Science

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“…Traditionally serving as architectural factors of chromatin, HMGBs play pivotal roles in transcriptional regulation, DNA repair, recombination, differentiation, extracellular signaling and nucleosome remodeling. 1,6 Recent discoveries illustrate that HMGBs, particularly HMGB1, function as cytokines or pro-inflammatory factors, playing a pivotal role in crosslinking innate and adaptive immunity. [7][8][9] Various studies have also highlighted the novel feature of HMGBs that function as universal sentinels for nucleic-acid-mediated innate immune responses.…”

Section: Introductionmentioning

confidence: 99%

Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

Rao

Yang

et al. 2014

Cell Mol Immunol

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High-mobility group box (HMGB) proteins, a family of chromatin-associated nuclear proteins, play amazingly multifaceted roles in the immune system of mammals. Thus far, little is known about the nucleocytoplasmic distribution of HMGBs in teleosts. The present study systematically investigated the dynamic localization of all six HMGB proteins in Ctenopharyngodon idella kidney (CIK) cells. Under basal conditions, all HMGBs exclusively localized to the nucleus. Grass carp reovirus (GCRV), polyinosinic-polycytidylic (poly(I : C)) potassium salt and lipopolysaccharide (LPS) challenge evoked the nuclear export of HMGBs to various degrees: GCRV challenge induced the highest nuclear export of CiHMGB2b, and poly(I : C) and LPS evoked the highest nucleocytoplasmic shuttling of CiHMGB1b. Overall, the nucleocytoplasmic shuttling of CiHMGB2a and CiHMGB3b was rarely induced by these challenges. Dynamic imaging uncovered that the nucleocytoplasmic GCRV-induced relocation of CiHMGB2b occurred in cells undergoing karyotheca rupture, apoptosis or proliferation. Western blot analyses were used to examine HMGB-EGFP fusion proteins in whole cell lysates, cytosol, nuclear fractions and culture medium. Further investigation demonstrated the nuclear retention of N-terminal HMG-boxes and the nucleocytoplasmic distribution of the C-terminal acidic tails. Comparative analyses of the dynamic relocation of full-length, truncated or chimeric HMGBs confirmed that the intramolecular interaction between HMG-boxes and C-tail domains mediated the nucleocytoplasmic translocation of HMGBs. These results not only provide an overall understanding of the subcellular localization of HMGBs, but also reveal the induction mechanism of the nucleocytoplasmic translocation of HMGBs by GCRV challenge, which lays a foundation for further studies on the interactions among pathogens, HMGBs and pattern recognition receptors in the innate immunity of teleosts. Keywords: grass carp (Ctenopharyngodon idella); grass carp reovirus; HMGBs; innate immunity; subcellular localization INTRODUCTION High-mobility group box (HMGB) proteins are abundant non-histone chromatin components implicated in major DNA transactions. In mammals, there are four family members (HMGB1-4), of which HMGB1, 2 and 3 are characterized by two DNA-binding domains (HMG-box A and B) and a Cterminal acidic tail domain and HMGB4 possesses two HMG-boxes but lacks the acidic tail. 1 In some teleosts, such as fugu (Takifugu), medaka (Oryzias latipes), Tetraodon and stickleback, two paralogous genes were detected for HMGB1 and HMGB2 but not for HMGB3. 2 However, in zebrafish (Danio rerio), salmon (Oncorhynchus), carp (Cyprinus carpio) 2 and grass carp (Ctenopharyngodon idella), 3-5 two paralogs are present within each of the HMGB subfamilies (HMGB1,

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“…Previous study showed that inflammation cytokine like high mobility group box 1 (HMGB1) was always associated with organ worsening [2] [4]. HMGB1 is a nuclear protein that has been known as transcription and growth factor [5] [6] [7].…”

Section: Introductionmentioning

confidence: 99%

The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury

Sirait¹,

Hatta²,

Ramli³

et al. 2017

OJAnes

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A severe injury can trigger an inflammation response and excessive response can cause multiple organ failure. HMGB1 is an early inflammation mediator in sterile injury and a late inflammation mediator in infection. It is an impor-tant mediator in severe sepsis and always associated with the severity of organ failure. Previous studies showed that the administration of systemic lidocaine could inhibit the expression of HMGB1 on septic mice with musculoskeletal injury. Nine male adult Balb/c mice were grouped by simple random sampling method into three groups of intravenous lidocaine injection dosages: 2 mg/kg, 3 mg/kg, 4 mg/kg. Musculoskeletal injury was done by breaking the left femo-ral bone in a close manner. Peripheral blood sampling was done 4 hours after injury and 2 hours after lidocaine therapy. To evaluate the expression level of HMGB1 mRNA, RT-PCR was used. The result of our study showed that intravenous lidoaine administration on the 3 groups could decrease the level of HMGB1. In conclusion, lidocaine hold an important role in clinical diseases by inhibiting HMGB1.

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HMGB proteins and transcriptional regulation

Cited by 125 publications

References 67 publications

HMGB2 regulates satellite-cell-mediated skeletal muscle regeneration through IGF2BP2

HMGB2 regulates satellite-cell-mediated skeletal muscle regeneration through IGF2BP2

Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury

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