Hollow fiber membrane diffusive permeability regulates encapsulated cell line biomass, proliferation, and small molecule release

Broadhead, Kelly W.; Biran, Roy; Tresco, Patrick A.

doi:10.1016/s0142-9612(02)00212-0

Cited by 33 publications

(20 citation statements)

References 37 publications

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“…While inward diffusion is critical for metabolic supply to the implanted cells, factors released by the capsule might elicit an immune response. Additionally, membrane permeability has been proposed to affect the quantity of recombinant proteins delivered by macroencapsulation implants [13]. We compared the long-term survival of luciferase-expressing cells encapsulated using two types of polypropylene membranes, with pore sizes of either 0.22 mm or 0.45 mm (Fig.…”

Section: Membrane Permeability and Initial Cell Densitymentioning

confidence: 99%

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Lathuilière¹,

Cosson²,

Lütolf³

et al. 2014

Biomaterials

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a b s t r a c tThe rapid increase in the number of approved therapeutic proteins, including recombinant antibodies, for diseases necessitating chronic treatments raises the question of the overall costs imposed on healthcare systems. It is therefore important to investigate alternative methods for recombinant protein administration. The implantation of genetically engineered cells is an attractive strategy for the chronic long-term delivery of recombinant proteins. Here, we have developed a high-capacity cell encapsulation system for the implantation of allogeneic myoblasts, which survive at high density for at least one year. This flat sheet device is based on permeable polypropylene membranes sealed to a mechanically resistant frame which confine cells seeded in a tailored biomimetic poly(ethylene glycol) (PEG)-based hydrogel matrix. In order to quantitate the number of cells surviving in the device and optimize initial conditions leading to high-density survival, we implant devices containing C2C12 mouse myoblasts expressing a luciferase reporter in the mouse subcutaneous tissue. We show that initial cell load, hydrogel stiffness and permeable membrane porosity are critical parameters to achieve long-term implant survival and efficacy. Optimization of these parameters leads to the survival of encapsulated myogenic cells at high density for several months, with minimal inflammatory response and dense neovascularization in the adjacent host tissue. Therefore, this encapsulation system is an effective platform for the implantation of genetically engineered cells in allogeneic conditions, which could be adapted to the chronic administration of recombinant proteins.

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Section: Membrane Permeability and Initial Cell Densitymentioning

confidence: 99%

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Lathuilière¹,

Cosson²,

Lütolf³

et al. 2014

Biomaterials

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“…In particular, membranes in tubular configurations act as guidance channels to protect the lumen of regenerating axons from the external environment. Surprisingly, among the variety of tubular structures used for guidance channels, semi‐permeable hollow‐fibre membranes appear to be considered a favourable guide for the regeneration of neuronal tissue (He et al , 2009; Broadhead et al , 2002; Zhang et al , 2005).…”

Section: Introductionmentioning

confidence: 99%

Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network

Morelli

Piscioneri

Salerno

et al. 2011

J Tissue Eng Regen Med

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The selection of appropriate biomaterials that promote cellular adhesion and growth is particularly important for the in vitro reconstruction of neuronal network. This study focused on the development of new polymeric membranes in flat and tubular (hollow-fibre) configurations as novel biomaterials for neuronal outgrowth. Two membrane systems constituted by modified polyetheretherketone (PEEK-WC) and polyacrylonitrile (PAN) membranes were developed and used for the culture of hamster hippocampal neurons. We demonstrated that all investigated membranes supported the adhesion and growth of hippocampal neurons enhancing neuronal differentiation and neurite alignment. The differences in cell behaviours between cells cultured on flat and hollow-fibre (HF) membranes were highlighted by the quantitative analysis of neuronal marker fluorescence intensity, morphometric analysis, RT-PCR analysis and also by metabolic activity measurements. In particular, the PAN HF membranes showed ideal growth culture conditions, guaranteeing adequate levels of metabolic features. Primary hippocampal cells cultured on PAN HF membranes were able to recreate in vitro a 3D neural tissue-like structure that, mimicking the hippocampal tissue, could be used as a tool for the study of natural and pathological neurobiological events.

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“…Poly(acrylonitrile‐ co ‐vinyl chloride) (PAN‐PVC) hollow fiber membranes were fabricated using a wet‐phase inversion process as previous described 13. Briefly, solutions of PAN‐PVC polymer in dimethysulphoxide (DMSO; Sigma Chemical Co.) were prepared by mixing 12.5 wt % PAN‐PVC and 87.5 wt % DMSO with constant stirring.…”

Section: Methodsmentioning

confidence: 99%

Development of a poloxamer analogs/bioadhesive polymers‐based in situ gelling ophthalmic delivery system for tiopronin

Jiang

Sun

Shen

et al. 2009

J of Applied Polymer Sci

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The purpose of this study was to develop a poloxamer analogs/bioadhesive polymers-based in situ gelling ophthalmic delivery system aiming at enhancing bioavailability and anticataract effect. The effect of poloxamer 407 (P407), poloxamer 188 (P188), carbopol 934P (C934), and sodium hyaluronate (NaHA) concentration on the gelation temperature (GT) was examined. The GT of P407 based in situ gel increased with an increase in the P188 concentration. NaHA and C934 lowered the GT of poloxamer analogs based in situ gel. Correlation analysis demonstrated that in vitro drug release from in situ gel was controlled by gel dissolution and followed zero-order kinetics. Tiopronin in vitro transcorneal transit accorded with zero-order kinetics. Twenty-two percent P407 and 6% P188 containing 0.2% NaHA based formulation can be chosen as in situ gel matrix of tiopronin because of proper GT and sustained releasing ability. In vivo study showed that the area under the aqueous humor-concentration time curve of tiopronin increased by 1.6 folds for in situ gel, compared with tiopronin aqueous solution. High-dose tiopronin in situ gel and solution delayed the development of selenite cataract 6 d and 4 d, respectively. The results showed that tiopronin in situ gel exhibits higher bioavailability and therapeutical effect.

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Hollow fiber membrane diffusive permeability regulates encapsulated cell line biomass, proliferation, and small molecule release

Cited by 33 publications

References 37 publications

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

A high-capacity cell macroencapsulation system supporting the long-term survival of genetically engineered allogeneic cells

Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network

Development of a poloxamer analogs/bioadhesive polymers‐based in situ gelling ophthalmic delivery system for tiopronin

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