Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds

Abstract

Background Although the treatments of skin wounds have greatly improved with the increase in therapeutic methods and agents, available interventions still cannot meet the current clinical needs. Therefore, the development of new pro-regenerative therapies remains urgent. Owing to their unique characteristics, both nanomaterials and peptides have provided novel clues for the development of pro-regenerative agents, however, more efforts were still be awaited and anticipated. Results In the current research, Hollow polydopamine (HPDA) nanoparticles were synthesized and HPDA nanoparticles loading with RL-QN15 (HPDAlR) that was an amphibian-derived peptide with obvious prohealing activities were prepared successfully. The characterization, biodistribution and clearance of both HPDA nanoparticles and HPDAlR were evaluated, the loading efficiency of HPDA against RL-QN15 and the slow-releasing rate of RL-QN15 from HPDAlR were also determined. Our results showed that both HPDA nanoparticles and HPDAlR exerted no obvious toxicity against keratinocyte, macrophage and mice, and HPDA nanoparticles showed no prohealing potency in vivo and in vitro. Interestingly, HPDAlR significantly enhanced the ability of RL-QN15 to accelerate the healing of scratch of keratinocytes and selectively modulate the release of healing-involved cytokines from macrophages. More importantly, in comparison with RL-QN15, by evaluating on animal models of full-thickness injured skin wounds in mice and oral ulcers in rats, HPDAlR showed significant increasing in the pro-regenerative potency of 50 and 10 times, respectively. Moreover, HPDAlR also enhanced the prohealing efficiency of peptide RL-QN15 against skin scald in mice and full-thickness injured wounds in swine. Conclusions HPDA obviously enhanced the pro-regenerative potency of RL-QN15 in vitro and in vivo, hence HPDAlR exhibited great potential in the development of therapeutics for skin wound healing. Graphic Abstract

Amphibian-derived peptide homodimer OA-GL17d promotes skin wound regeneration through the miR-663a/TGF-β1/Smad axis

“…Amphibian skin-secreted peptides are thus considered a valuable resource for the development of regenerative wound-healing agents due to their high affinity, high specificity, high safety and fast clearance rate. When combined with other treatment strategies, these peptides showed a strong pro-repair effect on skin wound regeneration [ 23 ]. Therefore, the discovery of new peptides has important clinical significance in skin wound repair.…”

“…The cytotoxic effects of OA-GL17d against HaCaT cells were analyzed using a Calcein-AM/PI Double Staining Kit [ 23 , 24 ]. Briefly, HaCaT cells were provided by the Kunming Cell Bank of Kunming Institute of Zoology, the Chinese Academy of Science (Yunnan, China).…”

“…Two dorsal-skin scald wounds were created by using a 5-ml centrifuge tube (inner diameter 12 mm) with the bottom cut off and flattened. The device was pressed onto the backs of the mice and boiling water was injected into the tube with a 2-ml syringe for 20 s to form skin scald wounds [ 23 , 31 ]. From days 0 to 14, scald skin wounds were topically treated with 20 μl of vehicle, OA-GL17d (0.5, 5 and 50 nM) or EGF (50 nM).…”

Short Hexapeptide Optimized from Rice-Derived Peptide 1 Shows Promising Anti-hyperuricemia Activities

“…Fluorescein isothiocyanate-labeled AAAAGA (FITC-labeled RDP1-M3) samples were obtained from Wuhan Biological Co., Ltd. (Wuhan, China) . The nude mice were treated with FITC-labeled RDP1-M3 (100 μL, 10 μg/μL) intraperitoneally.…”

“…Fluorescein isothiocyanate-labeled AAAAGA (FITC-labeled RDP1-M3) samples were obtained from Wuhan Biological Co., Ltd. (Wuhan, China). 16 The nude mice were treated with FITC-labeled RDP1-M3 (100 μL, 10 μg/μL) intraperitoneally. The images of ventral and dorsal of mice were taken at 15, 25, 35, 45, and 60 min after injection using a Bio-Real in vivo imaging system (IVIS Lumina Series III, PerkinElmer, USA).…”