Hollow silica microspheres as robust immobilization carriers

Snoch, Wojciech; Tataruch, Mateusz; Zastawny, Olga; Cichoń, Ewelina; Gosselin, Mathilde; Cabana, Hubert; Guzik, Maciej

doi:10.1016/j.bioorg.2019.02.038

Cited by 11 publications

(10 citation statements)

References 49 publications

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“…[19][20][21][22][23] Many methods of immobilization are described and used in the literature to circumvent the possible instability problems of the enzymes as well as to optimize the various applications. In recent years, the empirical use of these immobilization techniques (for example, covalent bonding, 24 physical adsorption, 25 ionic adsorption, 26 crosslinking, 27 encapsulation, 28 etc) and their influences on the specificity, activity and stability of the enzymatic molecules, as well as the usability of the biocatalysts for application-related [29][30][31] reactions. 29,[32][33][34][35] These immobilized enzymes, bound to the solid supports, can simply be removed from the reaction mixture.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

“…In recent years, the empirical use of these immobilization techniques (for example, covalent bonding, 24 physical adsorption, 25 ionic adsorption, 26 crosslinking, 27 encapsulation, 28 etc) and their influences on the specificity, activity and stability of the enzymatic molecules, as well as the usability of the biocatalysts for application-related [29][30][31] reactions. 29,[32][33][34][35] These immobilized enzymes, bound to the solid supports, can simply be removed from the reaction mixture. Thus, by minimizing the contamination of the final product containing the enzyme, it will be possible to reuse the recovered enzyme.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

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Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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The enzymatic processes are increasingly highlights, especially in the synthesis of chemical products with high added value. The enzyme immobilization can improve industrial biocatalytic processes. The immobilization of enzymes provides the production of efficient, stable biocatalysts, possibility of reuse and easy purification of the products, when compared to the free enzymes. There is a growing research for more efficient methods of enzyme immobilization. In this context, the choice of support and immobilization strategy can significantly improve the final enzymatic properties. In this review paper, we aimed to discuss the versatility of biocatalysts immobilized enzymes design, focusing on the opportunities and disadvantages for each method presented. They discussed the recent development of enzyme immobilization methods and applications relating the final properties of the produced biocatalysts with the desired goals.

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Section: Enzyme Immobilizationmentioning

confidence: 99%

Section: Enzyme Immobilizationmentioning

confidence: 99%

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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“…In this regard, fabrication of new materials, as hollow silica microspheres, allows achieving high protein loads and high flow rates without suffering pressure drops [29].…”

Section: Introductionmentioning

confidence: 99%

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Bolívar

López‐Gallego

2020

Current Opinion in Green and Sustainable Chemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…In MATSPHERE‐RES®‐NH 2 , electron acceptor depletion takes place due to the reduction mechanism caused by the reactive amino groups. This phenomenon is reported to cause a reduction in the activity of the enzyme immobilized on MATSPHERE‐RES®‐NH 2 .…”

Section: Carrier Materialsmentioning

confidence: 99%

Immobilization of Lipases – A Review. Part II: Carrier Materials

Thangaraj

Solomon

2019

ChemBioEng Reviews

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Carriers used in the immobilization process play a major role in enhancing the properties of the biocatalyst. Polymers are used as organic carriers for enzyme immobilization to improve the thermal, chemical, and operational stability of the enzyme. A variety of carriers are used in the immobilization of enzymes, e.g., mica, silica, zeolites, hydrotalcites, activated carbon, gold and magnetic nanoparticles. Silica‐based carriers offer suitable matrices for enzyme immobilization to manufacture industrial products. Immobilization on nanoparticles has become attractive in biocatalytic applications due to the combination of physical, chemical, catalytic, electronic, and optical properties. Gold nanoparticles also have received attention in the preparation of biocatalysts due to the above‐mentioned reasons. Magnetic nanoparticles are used as a carrier since they can be isolated by an external magnetic field, which is of special interest in the synthesis of organic products such as biodiesel. Different carrier materials involved in the immobilization of enzymes are discussed in this paper.

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Hollow silica microspheres as robust immobilization carriers

Cited by 11 publications

References 49 publications

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Immobilization of Lipases – A Review. Part II: Carrier Materials

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