Homemade bread: Repurposing an ancient technology for in vitro tissue engineering

Holmes, Jessica T.; Jaberansari, Ziba; Collins, W. M.; Latour, Maxime Leblanc; Modulevsky, Daniel J.; Pelling, Andrew E.

doi:10.1016/j.biomaterials.2021.121267

Cited by 25 publications

(10 citation statements)

References 66 publications

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“…Plant-derived scaffolds have demonstrated their efficiency for tissue engineering applications (Bilirgen et al, 2021;Cheng et al, 2020;Contessi Negrini et al, 2020;Dikici et al, 2019;Fontana et al, 2017;Gershlak et al, 2017;Harris et al, 2021;Hickey et al, 2018;Holmes et al, 2022;Lee et al, 2019;Modulevsky et al, 2016Modulevsky et al, , 2014Robbins et al, 2020;S. H et al, 2021;Toker et al, 2020;Wang et al, 2020;Zhu et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Mechanosensitive Osteogenesis on Native Cellulose Scaffolds for Bone Tissue Engineering

Pelling

2021

Preprint

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Accurate physical representation of the tissue microenvironment is essential for implant development. In this study, we applied cyclic hydrostatic pressure (HP) to mimic the effect of cyclic hydrostatic pressure (HP) in a bone-like environment, using a custom-made, remote controlled bioreactor. In recent years, plant-derived cellulosic biomaterials have become a popular way to create scaffolds for a variety of tissue engineering applications. Moreover, such scaffolds possess similar physical properties (porosity, stiffness) that resemble bone tissues and have been explored as potential biomaterials for tissue engineering applications. Here, plant-derived cellulose scaffolds (derived from apple hypanthium tissue) were seeded with MC3T3-E1 pre-osteoblast cells. After 1 week of proliferation, cell-seeded scaffolds were exposed to HP up to 270 KPa at a frequency of 1Hz, once per day, for up to 2 weeks. Scaffolds were incubated in osteogenic inducing media or regular culture media. The effect of cyclic hydrostatic pressure combined with osteogenic inducing media on cell-seeded scaffolds resulted in an increase of differentiated cells. This corresponded with an upregulation of alkaline phosphatase activity and scaffold mineralization. The results reveal that in vitro, the mechanosensitive pathways which regulate osteogenesis appear to be functional on novel plant-derived cellulosic biomaterials.

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Section: Discussionmentioning

confidence: 99%

Mechanosensitive Osteogenesis on Native Cellulose Scaffolds for Bone Tissue Engineering

Pelling

2021

Preprint

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“…As for their composition, scaffolds can be made from synthetic polymers or natural edible polymers. The latter can be animal-derived [ 29 ], plant-derived [ 102 , 105 , 106 ], fungi-derived, algae-derived [ 107 ] or composites of different polymers [ 108 ].…”

Section: Major Cell Types With Potential For Use In Cultivated Meat (...mentioning

confidence: 99%

Bioengineering Outlook on Cultivated Meat Production

et al. 2022

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Cultured meat (also referred to as cultivated meat or cell-based meat)—CM—is fabricated through the process of cellular agriculture (CA), which entails application of bioengineering, i.e., tissue engineering (TE) principles to the production of food. The main TE principles include usage of cells, grown in a controlled environment provided by bioreactors and cultivation media supplemented with growth factors and other needed nutrients and signaling molecules, and seeded onto the immobilization elements—microcarriers and scaffolds that provide the adhesion surfaces necessary for anchor-dependent cells and offer 3D organization for multiple cell types. Theoretically, many solutions from regenerative medicine and biomedical engineering can be applied in CM-TE, i.e., CA. However, in practice, there are a number of specificities regarding fabrication of a CM product that needs to fulfill not only the majority of functional criteria of muscle and fat TE, but also has to possess the sensory and nutritional qualities of a traditional food component, i.e., the meat it aims to replace. This is the reason that bioengineering aimed at CM production needs to be regarded as a specific scientific discipline of a multidisciplinary nature, integrating principles from biomedical engineering as well as from food manufacturing, design and development, i.e., food engineering. An important requirement is also the need to use as little as possible of animal-derived components in the whole CM bioprocess. In this review, we aim to present the current knowledge on different bioengineering aspects, pertinent to different current scientific disciplines but all relevant for CM engineering, relevant for muscle TE, including different cell sources, bioreactor types, media requirements, bioprocess monitoring and kinetics and their modifications for use in CA, all in view of their potential for efficient CM bioprocess scale-up. We believe such a review will offer a good overview of different bioengineering strategies for CM production and will be useful to a range of interested stakeholders, from students just entering the CA field to experienced researchers looking for the latest innovations in the field.

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“…In contrast, decellularized scaffolds can truly solve this challenge. A large number of plant tissues even have been shown to support the growth of a wide range of mammalian cell types [ 31 ] ( Table 1 ).…”

Section: Challenges Of Cell-cultured Meat Bioprocessingmentioning

confidence: 99%

Bioprocessing by Decellularized Scaffold Biomaterials in Cultured Meat: A Review

Ying

Shi

et al. 2022

Bioengineering

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As novel carrier biomaterials, decellularized scaffolds have promising potential in the development of cellular agriculture and edible cell-cultured meat applications. Decellularized scaffold biomaterials have characteristics of high biocompatibility, bio-degradation, biological safety and various bioactivities, which could potentially compensate for the shortcomings of synthetic bio-scaffold materials. They can provide suitable microstructure and mechanical support for cell adhesion, differentiation and proliferation. To our best knowledge, the preparation and application of plant and animal decellularized scaffolds have not been summarized. Herein, a comprehensive presentation of the principles, preparation methods and application progress of animal-derived and plant-derived decellularized scaffolds has been reported in detail. Additionally, their application in the culture of skeletal muscle, fat and connective tissue, which constitute the main components of edible cultured meat, have also been generally discussed. We also illustrate the potential applications and prospects of decellularized scaffold materials in future foods. This review of cultured meat and decellularized scaffold biomaterials provides new insight and great potential research prospects in food application and cellular agriculture.

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Homemade bread: Repurposing an ancient technology for in vitro tissue engineering

Cited by 25 publications

References 66 publications

Mechanosensitive Osteogenesis on Native Cellulose Scaffolds for Bone Tissue Engineering

Mechanosensitive Osteogenesis on Native Cellulose Scaffolds for Bone Tissue Engineering

Bioengineering Outlook on Cultivated Meat Production

Bioprocessing by Decellularized Scaffold Biomaterials in Cultured Meat: A Review

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