Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

Elnagar, Mohamed; Ibrahim, Mohamed F.; Albert, Magdy; M.Talal, Maya; Abdelfattah, Mahmoud M.; El-Dabaa, Ehab; Helwa, Reham

doi:10.1186/s13568-022-01483-x

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…The other side of the current situation is the delays in shipping of laboratories’ purchases. In the last two years, several research projects were carried out in our lab to make homemade solutions, in order to compromise with need gap (Abdelfattah et al 2022 ; Elnagar et al 2022 ; Mahmoud et al 2022 ). Hence, the aim of the present work is to make feasible homemade phenol-based reagents for RNA purification.…”

Section: Discussionmentioning

confidence: 99%

Back to Old Books Toward Affordable Research: Homemade Phenol-Based Reagent for Triphasic RNA Purification

Abdelfattah,

Hosny,

Elkady

et al. 2023

Biochem Genet

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Covid-19 crisis did hit many socio-economic aspects in the whole world. In the scientific research, the problem is getting even worse, since most of materials and consumable are allocated to the health sector. Many research laboratories around the world have big delay in receiving their purchases to accomplish their research projects. In the developing countries, the situation is much more difficult, since most of the funding resources are directed to the Covid-19 crisis and there is a notable increase in reagents’ prices. Therefore, the aim of the present study is to make a homemade reagents for RNA purification from eukaryotic cells/tissues. The homemade phenol-based RNA extraction reagents were prepared using saturated phenol pH 4.3 (adjusted by 0.5 M citrate buffer) and guanidine thiocyanate. To validate the phenol-based reagent, RNA was purified from different biological samples (cell line, tissues, and fungi) using homemade phenol-based versus a commercial one. Concentration of RNA samples extracted from the same number of cells were compared to assess the homemade preparation of phenol-based reagent. In conclusion, homemade phenol-based reagent is cost effective and comparable to the commercial one. Using homemade phenol-based, RNA extraction was successfully purified from different biological sources.

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Section: Discussionmentioning

confidence: 99%

Back to Old Books Toward Affordable Research: Homemade Phenol-Based Reagent for Triphasic RNA Purification

Abdelfattah,

Hosny,

Elkady

et al. 2023

Biochem Genet

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Making gene editing accessible in resource limited environments: recommendations to guide a first-time user

Goolab,

Scholefield

2024

Front. Genome Ed.

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The designer nuclease, CRISPR-Cas9 system has advanced the field of genome engineering owing to its programmability and ease of use. The application of these molecular scissors for genome engineering earned the developing researchers the Nobel prize in Chemistry in the year 2020. At present, the potential of this technology to improve global challenges continues to grow exponentially. CRISPR-Cas9 shows promise in the recent advances made in the Global North such as the FDA-approved gene therapy for the treatment of sickle cell anaemia and β-thalassemia and the gene editing of porcine kidney for xenotransplantation into humans affected by end-stage kidney failure. Limited resources, low government investment with an allocation of 1% of gross domestic production to research and development including a shortage of skilled professionals and lack of knowledge may preclude the use of this revolutionary technology in the Global South where the countries involved have reduced science and technology budgets. Focusing on the practical application of genome engineering, successful genetic manipulation is not easily accomplishable and is influenced by the chromatin landscape of the target locus, guide RNA selection, the experimental design including the profiling of the gene edited cells, which impacts the overall outcome achieved. Our assessment primarily delves into economical approaches of performing efficient genome engineering to support the first-time user restricted by limited resources with the aim of democratizing the use of the technology across low- and middle-income countries. Here we provide a comprehensive overview on existing experimental techniques, the significance for target locus analysis and current pitfalls such as the underrepresentation of global genetic diversity. Several perspectives of genome engineering approaches are outlined, which can be adopted in a resource limited setting to enable a higher success rate of genome editing-based innovations in low- and middle-income countries.

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Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

Cited by 2 publications

References 17 publications

Back to Old Books Toward Affordable Research: Homemade Phenol-Based Reagent for Triphasic RNA Purification

Back to Old Books Toward Affordable Research: Homemade Phenol-Based Reagent for Triphasic RNA Purification

Making gene editing accessible in resource limited environments: recommendations to guide a first-time user

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