Homogeneous osteogenesis and bone regeneration by demineralized bone matrix loading with collagen-targeting bone morphogenetic protein-2

Chen, Bing; Lin, Hang; Wang, Jianhua; Zhao, Yannan; Wang, Bin; Zhao, Wenxue; Sun, Wenjie; Dai, Jianwu

doi:10.1016/j.biomaterials.2006.10.013

Cited by 163 publications

(124 citation statements)

References 36 publications

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Section: In Vivo Engineering Of Bone Marrowsupporting

confidence: 79%

“…Histological analysis revealed that the marrow was largely inhabited by adipocytes and exhibited a low level of hematopoietic cell contribution, even 8 weeks after implantation (Fig. 1c), as previously noted by others using similar tissue engineering approaches with bone-inducing materials [19][20][21] .The presence of large numbers of adipocytes in bone marrow can inhibit hematopoiesis 23 . To reduce adipocyte content in the marrow, we sealed the top of central cavity in the implanted device by adding a solid layer of PDMS to restrict access of cells or soluble factors from the overlying adipocyte-rich hypodermis to the boneinducing materials, while maintaining accessibility to the underlying muscle tissue through the lower opening (Fig.…”

supporting

confidence: 74%

“…1a). We filled the hollow compartment with a type I collagen gel containing bone-inducing demineralized bone powder (DBP) and bone morphogenetic proteins (BMP2 and BMP4) [20][21][22] , and implanted it subcutaneously on the back of a mouse (Supplementary Fig. 1).…”

Section: In Vivo Engineering Of Bone Marrowmentioning

confidence: 99%

“…Tissue engineering methods have been used to induce formation of new bone with a central marrow compartment in vivo [18][19][20][21] . To explore the possibility of engineering an artificial bone marrow that can be explanted whole, we microfabricated a polydimethylsiloxane (PDMS) device with a central cylindrical cavity (1 mm high x 4 mm diameter) with openings at both ends (Fig.…”

Section: In Vivo Engineering Of Bone Marrowmentioning

confidence: 99%

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Bone marrow–on–a–chip replicates hematopoietic niche physiology in vitro

et al. 2014

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____________________________________________________________________________The bone marrow microenvironment contains a complex set of cellular, chemical, structural and physical cues necessary to maintain the viability and function of the hematopoietic system [1][2][3][4][5] . This hematopoietic niche regulates hematopoietic stem cells (HSCs), facilitating a delicate balance between self-renewal and differentiation into progenitor cells that produce all mature blood cell types 4,5 . Engineering an artificial bone marrow that reconstitutes natural marrow structure and function, and that can be maintained in culture, could be a powerful platform to study hematopoiesis and test new therapeutics. It has proven difficult, however, to recreate the complex bone marrow microenvironment needed to support the formation and maintenance of a complete, functional hematopoietic niche in vitro [6][7][8][9] . Although various in vitro culture systems have (Revised NMETH-A19608) been developed to maintain and expand hematopoietic stem and progenitor cells 6-11 , there is currently no method to recreate or study the intact bone marrow microenvironment in vitro. Therefore, studies on hematopoiesis commonly rely on animal models to ensure the presence of an intact bone marrow microenvironment that enables normal physiologic marrow responses [12][13][14][15] . Furthermore, although it has been reported that bone marrow can be engineered in vivo [16][17][18][19] , no method exists to culture engineered bone marrow in vitro. To bridge the functional gap between in vivo and in vitro systems, we developed a method to produce a "bone marrow-on-a-chip" culture system that contains artificial bone and a living marrow, which is first generated in mice, and then explanted whole and maintained in vitro within a microfluidic device. RESULTS In vivo engineering of bone marrowTissue engineering methods have been used to induce formation of new bone with a central marrow compartment in vivo [18][19][20][21] . To explore the possibility of engineering an artificial bone marrow that can be explanted whole, we microfabricated a polydimethylsiloxane (PDMS) device with a central cylindrical cavity (1 mm high x 4 mm diameter) with openings at both ends (Fig. 1a). We filled the hollow compartment with a type I collagen gel containing bone-inducing demineralized bone powder (DBP) and bone morphogenetic proteins (BMP2 and BMP4) [20][21][22] , and implanted it subcutaneously on the back of a mouse (Supplementary Fig. 1). Our goal was to engineer bone that would fill the cylindrical space within the implanted device so that it could be easily removed whole and inserted into a microfluidic system containing a similarly shaped (Revised NMETH-A19608) chamber for in vitro culture (Fig. 1 a,b). These initial studies resulted in the creation of new bone encasing a marrow compartment that formed within the PDMS device 4 to 8 weeks after subcutaneous implantation. Histological analysis revealed that the marrow was largely inhabited by adipocytes and exhibite...

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Section: In Vivo Engineering Of Bone Marrowsupporting

confidence: 79%

supporting

confidence: 74%

Section: In Vivo Engineering Of Bone Marrowmentioning

confidence: 99%

Section: In Vivo Engineering Of Bone Marrowmentioning

confidence: 99%

See 2 more Smart Citations

Bone marrow–on–a–chip replicates hematopoietic niche physiology in vitro

et al. 2014

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“…These biomaterials have been shown to facilitate the constructive remodeling of many different tissues in both preclinical animal studies and in human clinical applications, and they have been used in the field of abdominal wall reconstruction for many years. It has been reported that these biomaterials consist of a complex mixture of molecules that mediate structural and/ or biological properties, and also could effectively promoted cellular infiltration, new extracellular matrix deposition and neovascularization 2,3 . In addition, these biomaterials could become indistinguishable from native tissues.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

et al. 2014

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. Design of the study, critical revision. ABSTRACT PURPOSE:To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. METHODS:Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPRO TM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. RESULTS:After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. CONCLUSION:Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.Key words: Abdominal Wall. Biocompatible Materials. Intestinal, Fibroblast Growth Factor 2. Rats. Reconstruction of abdominal wall defects using small intestinal submucosa coated withgelatin hydrogel incorporating basic fibroblast growth factor

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Biologic Agents to Enhance Fracture Healing

Markel

Seeherman²

2019

Equine Fracture Repair

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Homogeneous osteogenesis and bone regeneration by demineralized bone matrix loading with collagen-targeting bone morphogenetic protein-2

Cited by 163 publications

References 36 publications

Bone marrow–on–a–chip replicates hematopoietic niche physiology in vitro

Bone marrow–on–a–chip replicates hematopoietic niche physiology in vitro

Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

Biologic Agents to Enhance Fracture Healing

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