Honeybees Exposure to Natural Feed Additives: How Is the Gut Microbiota Affected?

Alberoni, Daniele; Baffoni, Loredana; Braglia, Chiara; Gaggìa, Francesca; Gioia, Diana Di

doi:10.3390/microorganisms9051009

Cited by 15 publications

(17 citation statements)

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“…Moreover, species belonging to the Lactobacillus genus have been recently reclassified ( 76 ) but databases for NGS OTUs assignment were not yet updated with the new classification at the time of the bioinformatic analysis of the presented data. Therefore, the data set was manually adjusted according to Alberoni et al ( 77 ) in order to reassign former Lactobacillus sp. Firm-4 to the Bombilactobacillus spp.…”

Section: Methodsmentioning

confidence: 99%

Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?

et al. 2021

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Section: Methodsmentioning

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Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?

et al. 2021

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“…The total number of bacteria (Eubacteria) in the honeybee gut content was determined at day 9 (as reported in Section "2.4") via qPCR assay according to Alberoni et al [39], using the primers Eub338-F 3'ACT CCT ACG GGA GGC AGC AG-5' and Eub518-R 3'ATT ACC GCG GCT GCTGG-5'. After analysing the NGS data (see "Results" below), the genus Serratia was considered worthy of absolute quantification using qPCR.…”

Section: Quantitative Pcrmentioning

confidence: 99%

“…When compared to [C], all the other cage Fig.2NGS relative abundance normalized with qPCR on total bacteria according to Baffoni et al[24] (normalized relative abundance). Bar chart reporting the major cumulated microbial genera per sample and experimental condition: [C] honeybees fed with sugar syrup; (P) honeybees fed with sugar syrup enriched with BB mixture; [N] hon-eybees fed with sugar syrup and infected with N. ceranae; [NP] honeybees fed with sugar syrup, enriched with BB mixture and infected with N. ceranae; [FC] honeybees reared in field condition with sugar syrup supply as in Alberoni et al[39]. The OTUs below 0.1% are grouped in the category named "Others" experimental conditions showed a significant increase in…”

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Alterations in the Microbiota of Caged Honeybees in the Presence of Nosema ceranae Infection and Related Changes in Functionality

2022

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Several studies have outlined that changes in the honeybee gut microbial composition may impair important metabolic functions supporting the honeybees’ life. Gut dysbiosis may be caused by diseases like Nosema ceranae or by other anthropic, environmental or experimental stressors. The present work contributes to increasing knowledge on the dynamics of the gut microbiome acquisition in caged honeybees, an experimental condition frequently adopted by researchers, with or without infection with N. ceranae, and fed with a bacterial mixture to control N. ceranae development. Changes of the gut microbiota were elucidated comparing microbial profile of caged and open-field reared honeybees. The absolute abundance of the major gut microbial taxa was studied with both NGS and qPCR approaches, whereas changes in the functionality were based on RAST annotations and manually curated. In general, all caged honeybees showed important changes in the gut microbiota, with $$\gamma$$ γ -proteobacteria (Frischella, Gilliamella and Snodgrassella) lacking in all caged experimental groups. Caged honeybees infected with N. ceranae showed also a strong colonization of environmental taxa like Citrobacter, Cosenzaea and Morganella, as well as possibly pathogenic bacteria such as Serratia. The colonization of Serratia did not occur in presence of the bacterial mixture. The functionality prediction revealed that environmental bacteria or the supplemented bacterial mixture increased the metabolic potential of the honeybee gut microbiome compared to field and caged controls.

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“…До них належить наявність в організмах бджіл асоціації пробіотичних культур, формування якої залежить від видового складу медоносів, породності сімей, технологічних чинників їх утримання, стану навколишнього середовища та ін. (Nazarenko & Yevstafieva, 2019;Alberoni et al, 2021).…”

Section: обговоренняunclassified

Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

Postoienko¹,

Postoienko²,

Yefimenko³

et al. 2022

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Viral diseases of bees, in particular the sac brood, are an urgent problem of the industry, which brings great losses. The situation worsens in the case of parallel infestation of insects by the Varroa mite. Today, a complex approach is used for the treatment of viral diseases of bees, which is based on a connection of antiviral and antiparasitic measures in combination with drugs that have general strengthening and biostimulating effects. Nevertheless, the issue of developing ecologically safe and effective schemes for the prevention and treatment of bee diseases, in particular viral ones, is relevant today. In this work, the positive effect of using a specific probiotic for bees “Apinormin” for sac brood was investigated. Research in laboratory conditions was carried out on bees of the summer generation, selected into cages. The oral effect of the drug was determined by feeding bees with sugar syrup, the contact effect by spraying the body of insects with an aqueous solution in concentrations of 0.4; 0.8; 4.0; 8.0 %. Under natural conditions, the therapeutic effectiveness of a 4 % solution of “Apinormin” compared to the creation of an infertile period for the period of removal of queens for bee sac brood was determined. In laboratory conditions, the drug was proven to be harmless for bees when used orally in all tested concentrations. It has been established that “Apinormin” significantly increases the life expectancy of insects. Thus, feeding an 8 % probiotic solution slowed down the process of natural dying of bees from 7.24 to 27.75 % in the dynamics of observations. Under natural conditions, the drug did not show an antiviral effect. The effectiveness of the infertile period in the treatment of this viral disease is shown. The obtained positive effect of “Apinormin” on the life extension of bees and the presence of a number of biologically active compounds in its composition serve as a basis for the use of the drug to increase the strength and productivity of families, in case of stress, protein starvation, various technological violations of maintenance and the development of schemes for the prevention and treatment of bacterial diseases of bees. The expediency of using the infertile period for the period of removal of the queens in the following studies, both for self-administration and for working out the schemes of complex therapy of infectious diseases of bees together with the appropriate veterinary drugs, is substantiated.

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Honeybees Exposure to Natural Feed Additives: How Is the Gut Microbiota Affected?

Cited by 15 publications

References 60 publications

Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?

Honeybee Exposure to Veterinary Drugs: How Is the Gut Microbiota Affected?

Alterations in the Microbiota of Caged Honeybees in the Presence of Nosema ceranae Infection and Related Changes in Functionality

Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

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