Hookworm infection in central China: morphological and molecular diagnosis

Xu, Fang; Niu, Yu Fei; Chen, Wen Qing; Liu, Shasha; Li, Jing Ru; Jiang, Peng; Wang, Zhong Quan; Cui, Jing; Zhang, Xi

doi:10.1186/s13071-021-05035-3

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…In coproparasitological studies, species identification may be problematic due to the low discriminatory value of characteristics of hookworm eggs. In addition, the accurate species identification of hookworms is difficult when the collected specimens are incomplete, especially when key taxonomic characters in the head or tail are damaged (Xu et al, 2021). In contrast, the genotypic characterization using several target genes has been useful for identification of parasite species.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Ancylostoma caninum from a gray fox Urocyon cinereoargenteus in southeastern Mexico

et al. 2022

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Summary The hookworm Ancylostoma caninum is a common nematode of wild and domestic canids worldwide. In Mexico, there are few records of helminths in wild canids, especially in the southeastern region. The aim of the present study was to examine the helminths from a gray fox Urocyon cinereoargenteus in southeastern Mexico. A road-killed female gray fox found in Merida, Yucatan, Mexico, was examined for helminths. Only nematodes were found in the intestine of the gray fox and identified using morphological studies and molecular analysis of 28S rRNA gene fragments. The characteristics exhibited by the nematode specimens were in accordance with descriptions of A. caninum: e. g. oral opening with a pair of prominent chitinous plates bearing three pairs of ventral teeth, lateral rays with a common trunk, dorsal ray divided into two branches with each branch terminating in three digitations. BLAST analysis of the 28S sequence showed similarity and coverage values of 99.8 % and 100 %, respectively, with a sequence of A. caninum from the domestic dog Canis familiaris in Australia. The genetic distance between the Australian specimen and the Yucatan specimen of A. caninum was 0.1 %, that is, they were only different in a single nucleotide. The gray fox examined in this study was found close to a rural community where A. caninum has been recorded from domestic dogs, which could be the source of infection. Our study increases the distribution of this nematode parasitizing the gray fox in Mexico and provides the first nucleotide sequence of A. caninum from the gray fox.

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Section: Resultsmentioning

confidence: 99%

Occurrence of Ancylostoma caninum from a gray fox Urocyon cinereoargenteus in southeastern Mexico

et al. 2022

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“…In this sense, genetic data can be an information source for the diagnosis and taxonomy of helminths, especially for those groups that present complications in their systematics (e.g. Dicrocoelidae) (Poulin et al ., 2019 ; Suleman et al ., 2020 ) or when key taxonomic characters cannot be studied due to insufficient or incomplete material (Xu et al ., 2021 ). Our results highlight the importance of complementing morphological identification of helminths of Neotropical bats with molecular tools.…”

Section: Discussionmentioning

confidence: 99%

Survey on helminths of bats in the Yucatan Peninsula: infection levels, molecular information and host–parasite networks

et al. 2022

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Helminth species of Neotropical bats are poorly known. In Mexico, few studies have been conducted on helminths of bats, especially in regions such as the Yucatan Peninsula where Chiroptera is the mammalian order with the greatest number of species. In this study, we characterized morphologically and molecularly the helminth species of bats and explored their infection levels and parasite–host interactions in the Yucatan Peninsula, Mexico. One hundred and sixty-three bats (representing 21 species) were captured between 2017 and 2022 in 15 sites throughout the Yucatan Peninsula. Conventional morphological techniques and molecular tools were used with the 28S gene to identify the collected helminths. Host–parasite network analyses were carried out to explore interactions by focusing on the level of host species. Helminths were found in 44 (26.9%) bats of 12 species. Twenty helminth taxa were recorded (7 trematodes, 3 cestodes and 10 nematodes), including 4 new host records for the Americas. Prevalence and mean intensity of infection values ranged from 7.1 to 100% and from 1 to 56, respectively. Molecular analyses confirmed the identity of some helminths at species and genus levels; however, some sequences did not correspond to any of the species available on GenBank. The parasite–host network suggests that most of the helminths recorded in bats were host-specific. The highest helminth richness was found in insectivorous bats. This study increases our knowledge of helminths parasitizing Neotropical bats, adding new records and nucleotide sequences.

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“…Further digital images remain available for referencing and auditing purposes. On the other hand, molecular techniques such as qPCR exhibit high sensitivity and can differentiate human and animal species based on their DNA/RNA sequences (Dos Santos et al 2022 ; Furtado et al 2020 ; Ngcamphalala et al 2020 ; Palma et al 2019 ; Xu et al 2021 ; Zhou et al 2021 ), significantly improving diagnostic sensitivity and offering the prospect of diagnosing even very low-level infection intensities (Benjamin-Chung et al 2020 ; Dunn et al 2020 ). This enables better monitoring of parasite infections and their response to treatment.…”

Section: One-health Approach To Anthelmintic Drug Resistancementioning

confidence: 99%

Anthelmintic resistance in soil-transmitted helminths: One-Health considerations

Ng’etich,

Amoah,

Bux

et al. 2023

Parasitol Res

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The One-Health approach recognizes the intricate connection between human, animal, and environmental health, and that cooperative effort from various professionals provides comprehensive awareness and potential solutions for issues relating to the health of people, animals, and the environment. This approach has increasingly gained appeal as the standard strategy for tackling emerging infectious diseases, most of which are zoonoses. Treatment with anthelmintics (AHs) without a doubt minimizes the severe consequences of soil-transmitted helminths (STHs); however, evidence of anthelmintic resistance (AR) development to different helminths of practically every animal species and the distinct groups of AHs is overwhelming globally. In this regard, the correlation between the application of anthelmintic drugs in both human and animal populations and the consequent development of anthelmintic resistance in STHs within the context of a One-Health framework is explored. This review provides an overview of the major human and animal STHs, treatment of the STHs, AR development and drug-related factors contributing towards AR, One-Health and STHs, and an outline of some One-Health strategies that may be used in combating AR.

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Hookworm infection in central China: morphological and molecular diagnosis

Cited by 6 publications

References 22 publications

Occurrence of Ancylostoma caninum from a gray fox Urocyon cinereoargenteus in southeastern Mexico

Occurrence of Ancylostoma caninum from a gray fox Urocyon cinereoargenteus in southeastern Mexico

Survey on helminths of bats in the Yucatan Peninsula: infection levels, molecular information and host–parasite networks

Anthelmintic resistance in soil-transmitted helminths: One-Health considerations

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