Hop Resin Analysis by High-Performance Liquid Chromatography on Microparticulate Silica

A rapid reversed phase HPLC method for the analysis for a-and (5-acids in hops and hop products is described and has been evaluated. The method uses citric acid in the eluent as a complexing agent to overcome the irreversible adsorption effects shown by some columns, thus allowing optimum eluent pH to be selected. The precision of the method for analysis of hop extract has been deter mined giving relative standard deviations of 1 -0% and 2-1 % for a-and p-acids respectively. General agreement with results obtained using a polarimetric a-acids analysis method for hop extracts and hops has been demonstrated.

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SIMULTANEOUS DETERMINATION OF Α, Β AND ISO-α ACIDS IN HOPS AND HOP PRODUCTS

Silvester¹

1984

Journal of the Institute of Brewing

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A method for the simultaneous analysis of a, p* and iso-a acid in hops, hop extracts and isomerised hop extracts is described. It is based on the use of reversed phase high performance liquid chromatography and quantitative evaluation of the hop compounds is carried out with a computing integrator.The isomerisation reaction can be examined in detail, particularly in connection with the production of hop derived haze forming compounds in isomerised hop extracts used for post fermentation bittering.

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Hop Resin Analysis by High-Performance Liquid Chromatography on Microparticulate Silica

Cited by 14 publications

References 29 publications

Hop Extracts-a Review

Hop Extracts-a Review

The DETERMINATION OF Α- AND Β-Acids IN HOPS AND HOP PRODUCTS USING HPLC

SIMULTANEOUS DETERMINATION OF Α, Β AND ISO-α ACIDS IN HOPS AND HOP PRODUCTS

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