Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.)

Abstract

The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analyzed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis

Barley Varieties Discrimination Using Maldi-Tof Mass Spectrometry

“…The diversity in the hordein family has made the analysis of these fractions very useful in varietal identification. Such diversity may be explained through differences within the B and C hordeins that occur between varieties, as well as grain protein levels and environments (Fox et al 2003;Perovic et al 1998). The aim of this study was to identify barely varieties by using Matrix Assisted Laser Desoption/Ionization Time-Of-Flight Mass Spectrometry (MALDI TOF MS).…”