Hormone interactions confer specific proliferative and histomorphogenic responses in the porcine mammary gland

Horigan, Katherine C; Trott, Josephine F.; Barndollar, A.S.; Scudder, J.M.; Blauwiekel, Ruth; Hovey, Russell C.

doi:10.1016/j.domaniend.2009.04.002

Cited by 36 publications

(51 citation statements)

References 54 publications

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“…Consistent with our previous findings [14], the epithelial structures in the MG of control-fed pubertal female pigs included TDLU types 1-3, with a slightly higher abundance of type 1. After pigs were fed the alcohol-supplemented diet their MG had an increased proportion of TDLU2 (P = 0.02) that was accompanied by a corresponding decrease in the incidence of TDLU1 (P = 0.02).…”

Section: Resultssupporting

confidence: 91%

“…In a previous study we recorded that epithelial proliferation was induced synergistically by exogenous estrogen and prolactin [14]. Based on our unpublished microarray data, we examined whether the expression of genes that were up-or down-regulated by the combination of these hormones (SLC28A3, AK345252, AK235587, TNXB, ATP6V1C2 and c-jun) was also affected by alcohol consumption.…”

Section: Resultsmentioning

confidence: 99%

“…Morphological development of epithelial TDLU structures within the pig MG was determined as we previously described and validated [14], using criteria described for the human breast [22]. Briefly, a TDLU1 in the human breast is a simple collection of approximately 11 ductules, a TDLU2 contains an average of 47 ductules, and the largest and most complex TDLU3 structures contain approximately 81 ductules [22].…”

Section: Mammary Gland Assessmentsmentioning

confidence: 99%

“…Fixed tissues obtained from three MG, distributed across the entire mammary chain in each pig, were prepared as whole mounts, without acetone defatting, as described [14,21]. Carmine alum stained semi-thick sections (n = 3 per MG) were mounted in Permount (Thermo Fisher Scientific, Hampton, New Hampshire, USA) and imaged with a dissecting microscope (Olympus SZX16, Shinjuku, Tokyo, Japan).…”

Section: Mammary Gland Assessmentsmentioning

confidence: 99%

“…Given that the breast undergoes substantial development during adolescence in response to a changing endocrine environment [11], any increase in epithelial proliferation or exposure to transforming events during this window may increase the risk for developing breast cancer later in life [12,13]. The mammary glands (MG) of pigs represent an excellent model for the human breast given similarities in the histological and morphological features of the epithelium and stroma [14,15]. At the same time, pigs are genetically more similar to humans than mice [16] and have a similar physiology to humans [17].…”

mentioning

confidence: 99%

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Alcohol intake stimulates epithelial proliferation in an authentic model of the human breast

Schennink

Trott

Berryhill

et al. 2015

Reproductive Toxicology

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The voluntary consumption of alcohol by humans is a modifiable lifestyle factor that has been consistently linked to a woman's risk of developing breast cancer. We have used an animal model that closely recapitulates breast development in humans to study the effect of alcohol intake on breast growth and morphology. Pubertal female pigs were fed alcohol for 4-5 weeks at 19-21% of total caloric intake, which led to average blood alcohol concentrations of 115-130mg/dL. Alongside increased liver mass, alcohol intake promoted the formation of distended ductules within lobular units in association with increased epithelial proliferation. Alcohol consumption also increased phosphorylation of the transcription factor STAT5 in the mammary epithelium, but did not lead to any evidence of precocious lactogenesis. In conclusion, feeding alcohol to female pigs having a similar physiology and mammary gland morphology to humans during a reproductive state equivalent to human adolescence leads to increased mammary gland proliferation and development of atypical lobular structures. These changes may phenocopy how alcohol intake increases the risk for developing breast cancer in humans.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Mammary Gland Assessmentsmentioning

confidence: 99%

Section: Mammary Gland Assessmentsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Alcohol intake stimulates epithelial proliferation in an authentic model of the human breast

Schennink

Trott

Berryhill

et al. 2015

Reproductive Toxicology

View full text Add to dashboard Cite

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Development and Pathology of the Equine Mammary Gland

Hughes

2020

J Mammary Gland Biol Neoplasia

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An understanding of the anatomy, histology, and development of the equine mammary gland underpins study of the pathology of diseases including galactorrhoea, agalactia, mastitis, and mammary tumour development. This review examines the prenatal development of the equine mammary gland and the striking degree to which the tissue undergoes postnatal development associated with the reproductive cycle. The gland is characterised by epithelial structures arranged in terminal duct lobular units, similar to those of the human breast, supported by distinct zones of intra- and interlobular collagenous stroma. Mastitis and mammary carcinomas are two of the most frequently described equine mammary pathologies and have an overlap in associated clinical signs. Mastitis is most frequently associated with bacterial aetiologies, particularly Streptococcus spp., and knowledge of the process of post-lactational regression can be applied to preventative husbandry strategies. Equine mammary tumours are rare and carry a poor prognosis in many cases. Recent studies have used mammosphere assays to reveal novel insights into the identification and potential behaviour of mammary stem/progenitor cell populations. These suggest that mammospheres derived from equine cells have different growth dynamics compared to those from other species. In parallel with studying the equine mammary gland in order to advance knowledge of equine mammary disease at the interface of basic and clinical science, there is a need to better understand equine lactational biology. This is driven in part by the recognition of the potential value of horse and donkey milk for human consumption, particularly donkey milk in children with ‘Cow Milk Protein Allergy’.

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In Utero Exposure to trans-10, cis-12 Conjugated Linoleic Acid Modifies Postnatal Development of the Mammary Gland and its Hormone Responsiveness

Berryhill

Gloviczki

Trott

et al. 2021

J Mammary Gland Biol Neoplasia

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We previously showed that dietary trans-10, cis-12 conjugated linoleic acid (10,12 CLA) stimulates estrogen-independent mammary growth in young ovariectomized mice. Here we investigated the effects of in utero or postnatal exposure to cis-9, trans-11 (9,11 CLA) and 10,12 CLA on postnatal development of the mammary gland and its responsiveness to ovarian steroids. In the first experiment we fed dams different CLA prior to and during gestation, then cross fostered female pups onto control fed dams prior to assessing the histomorphology of their mammary glands. Pregnant dams in the second experiment were similarly exposed to CLA, after which their female pups were ovariectomized then treated with 17β-estradiol (E), progesterone (P) or E + P for 5 days. In a third experiment, mature female mice were fed different CLA for 28 days prior to ovariectomy, then treated with E, P or E + P. Our data indicate that 10,12 CLA modifies the responsiveness of the mammary glands to E or E + P when exposure occurs either in utero, or postnatally. These findings underline the sensitivity of the mammary glands to dietary fatty acids and reinforce the potential for maternal nutrition to impact postnatal development of the mammary glands and their risk for developing cancer.

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Hormone interactions confer specific proliferative and histomorphogenic responses in the porcine mammary gland

Cited by 36 publications

References 54 publications

Alcohol intake stimulates epithelial proliferation in an authentic model of the human breast

Alcohol intake stimulates epithelial proliferation in an authentic model of the human breast

Development and Pathology of the Equine Mammary Gland

In Utero Exposure to trans-10, cis-12 Conjugated Linoleic Acid Modifies Postnatal Development of the Mammary Gland and its Hormone Responsiveness

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