Hormones and Flower Development in Arabidopsis

Zúñiga-Mayo, Víctor M.; Durán-Medina, Yolanda; Marsch-Martínez, Nayelli; Folter, Stefan de

doi:10.1007/978-1-0716-3299-4_5

Cited by 3 publications

(1 citation statement)

References 122 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present transcriptomic data revealed that MLP28 was downregulated in stk funiculi, which was confirmed by qPCR and promoter-driven GUS activity assays. Due to MLPs nature of binding to plant hormones already implicated in several processes of reproduction (Zuniga-Mayo et al, 2023), it seems likely their involvement with ligand transportation from the funiculus to the seed. The last gene selected was SCPL41, a serine carboxypeptidase-like protein containing a catalytic triad responsible for cleaving the C-terminal peptide bond in proteins or peptides (Fraser et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic landscape ofseedstickinArabidopsis thalianafuniculus after fertilisation

Ferreira,

Silva,

Takeuchi

et al. 2023

Preprint

View full text Add to dashboard Cite

In Angiosperms, the continuation of plant species is intricately dependent on the funiculus multifaceted role in nutrient transport, mechanical support, and dehiscence of seeds. SEEDSTICK (STK) is a MADS-box transcription factor involved in seed size and dehiscence, and one of the few genes identified as affecting funiculus growth. Given the importance of the funiculus to a correct seed development, allied with previous phenotypic observations ofstkmutants, we performed a transcriptomic analysis ofstkfuniculi, using RNA-sequencing, to infer on the deregulated networks of genes. The generated dataset of differentially expressed genes was enriched with cell wall biogenesis, cell cycle, sugar metabolism and transport terms, all in accordance withstkphenotype. We selected eight differentially expressed genes involved with abscission, seed development or novel functions instkfuniculus, such as hormones/secondary metabolites transport, for transcriptome validation using qPCR and/or promoter reporter lines. Overall, the analysis performed in this study allowed delving into the STK-network established in Arabidopsis funiculus, fulfilling a literature gap. Simultaneously, our findings reinforced the reliability of the transcriptome, and identified processes and new candidate genes that will enable a better understanding on the role of this sporophytic structure and how seed development may be affected by it.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomic landscape ofseedstickinArabidopsis thalianafuniculus after fertilisation

Ferreira,

Silva,

Takeuchi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Natural variations in the Cis‐elements of GhRPRS1 contributing to petal colour diversity in cotton

Hu,

Chen,

et al. 2024

Plant Biotechnology Journal

View full text Add to dashboard Cite

SummaryThe cotton genus comprises both diploid and allotetraploid species, and the diversity in petal colour within this genus offers valuable targets for studying orthologous gene function differentiation and evolution. However, the genetic basis for this diversity in petal colour remains largely unknown. The red petal colour primarily comes from C, G, K, and D genome species, and it is likely that the common ancestor of cotton had red petals. Here, by employing a clone mapping strategy, we mapped the red petal trait to a specific region on chromosome A07 in upland cotton. Genomic comparisons and phylogenetic analyses revealed that the red petal phenotype introgressed from G. bickii. Transcriptome analysis indicated that GhRPRS1, which encodes a glutathione S‐transferase, was the causative gene for the red petal colour. Knocking out GhRPRS1 resulted in white petals and the absence of red spots, while overexpression of both genotypes of GhRPRS1 led to red petals. Further analysis suggested that GhRPRS1 played a role in transporting pelargonidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside. Promoter activity analysis indicated that variations in the promoter, but not in the gene body of GhRPRS1, have led to different petal colours within the genus. Our findings provide new insights into orthologous gene evolution as well as new strategies for modifying promoters in cotton breeding.

show abstract

Transcriptomic landscape of seedstick in Arabidopsis thaliana funiculus after fertilisation

Ferreira,

Silva,

Takeuchi

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background In Angiosperms, the continuation of plant species is intricately dependent on the funiculus multifaceted role in nutrient transport, mechanical support, and dehiscence of seeds. SEEDSTICK (STK) is a MADS-box transcription factor involved in seed size and abscission, and one of the few genes identified as affecting funiculus growth. Given the importance of the funiculus to a correct seed development, allied with previous phenotypic observations of stk mutants, we performed a transcriptomic analysis of stk funiculi from floral stage 17, using RNA-sequencing, to infer on the deregulated networks of genes. Results The generated dataset of differentially expressed genes was enriched with cell wall biogenesis, cell cycle, sugar metabolism and transport terms, all in accordance with stk phenotype observed in funiculi from floral stage 17. We selected eight differentially expressed genes for transcriptome validation using qPCR and/or promoter reporter lines. Those genes were involved with abscission, seed development or novel functions in stk funiculus, such as hormones/secondary metabolites transport. Conclusion Overall, the analysis performed in this study allowed delving into the STK-network established in Arabidopsis funiculus, fulfilling a literature gap. Simultaneously, our findings reinforced the reliability of the transcriptome, making it a valuable resource for candidate genes selection for functional genetic studies in the funiculus. This will enhance our understanding on the regulatory network controlled by STK, on the role of the funiculus and how seed development may be affected by them. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-024-05489-4.

show abstract

Hormones and Flower Development in Arabidopsis

Cited by 3 publications

References 122 publications

Transcriptomic landscape ofseedstickinArabidopsis thalianafuniculus after fertilisation

Transcriptomic landscape ofseedstickinArabidopsis thalianafuniculus after fertilisation

Natural variations in the Cis‐elements of GhRPRS1 contributing to petal colour diversity in cotton

Transcriptomic landscape of seedstick in Arabidopsis thaliana funiculus after fertilisation

Contact Info

Product

Resources

About