Horseradish‐root‐modified carbon paste bioelectrode

Wang, Joseph; Lin, Min-Sheng

doi:10.1002/elan.1140010107

Cited by 77 publications

(19 citation statements)

References 5 publications

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“…O primeiro eletrodo de pasta de carbono 8 foi desenvolvido, em 1958, na tentativa de preparar um eletrodo de carbono renovável para ser usado em uma ampla faixa de potenciais positivos onde o eletrodo de mercúrio metálico não é aplicável devido à sua oxidação. No entanto, o primeiro eletrodo de pasta de carbono modificado com enzima (glicose oxidase) foi proposto apenas no final da déca-da de 80 9 , e desde então vários biossensores foram desenvolvidos usando diversos materiais biológicos [10][11][12][13][14][15][16] . A pasta de carbono é uma mistura de pó de grafite e aglutinante.…”

Section: Introductionunclassified

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Vieira¹,

Lupetti²,

Fatibello‐Filho³

2003

Quím. Nova

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were investigated as the source of peroxidase (POD: EC 1.11.1.7). Among those, zucchini (Cucurbita pepo) crude extract was found to be the best one. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetylp-benzoquinoneimine which the electrochemical reduction back to paracetamol was obtained at a peak potential of -0.10V. A cyclic voltammetric study was performed by scanning the potential from + 0.5 to -0.5 V. The recovery of paracetamol from two samples ranged from 97.3 to 106% and a rectilinear calibration curve for paracetamol concentration from 1.2x10 -4 to 2.5x10 -3 mol L -1 (r=0.9965) were obtained. The detection limit was 6.9x10 -5 mol L -1 and the relative standard deviation was less than 1.1% for a solution containing 2.5x10 -3 mol L -1 paracetamol and 2.0x10 -3 mol L -1 hydrogen peroxide (n=12). The results obtained for paracetamol in pharmaceutical products using the proposed biosensor and Pharmacopoeial procedures are in agreement at the 95% confidence level.

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Section: Introductionunclassified

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Vieira¹,

Lupetti²,

Fatibello‐Filho³

2003

Quím. Nova

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“…Tissues of several plants such as asparagus, 38,39 grape, 40 pineapple, 41 kohlrabi, 42 tobacco callus, 43 horseradish roots, 44,45 turnip 46 and sweet potato 47 have been used as POx sources. Vieira and Fatibello-Filho 48 have chosen the crude extract of zucchini as a POx source of very high specific activity and have used it for the spectrophotometric determination of H2O2.…”

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confidence: 99%

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

2006

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Crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared by a simple procedure and its enzymatic activity and total protein concentration were determined. It was found that this crude extract is a rich source of peroxidase (POx) and has high specific activity. Cross-linked polyvinylpyrrolidone was used as a stabilizer in the preparation of the crude extract. The POx activity of kohlrabi crude extract did not vary for at least 2 months when deoxygenated and stored at 4˚C. This extract was applied for the spectrofluorometric determination of hydrogen peroxide using homovanillic acid as a fluorogenic substrate. POx catalyzes the hydrogen peroxide oxidation of homovanillic acid to produce a dimer which shows strong fluorescence at 420 nm with excitation at 312 nm. In the optimum conditions, the calibration graph for hydrogen peroxide was linear up to 190 ng mL -1 , with a detection limit of 4.4 ng mL -1 . The relative standard deviation (RSD) was 1.48% for 50 ng mL -1 hydrogen peroxide. The proposed method was successfully applied to the determination of hydrogen peroxide in honey. The concentration-time profile of H2O2 produced upon dilution of honey was studied and H2O2 contents of some different honeys from various areas of Iran were determined.

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“…The tissue thus becomes an integral part of the sensing element, resulting in extremely short response times. The amperometric detection of dopamine [31], hydrogen peroxide [32], oxalate [33], ethanol [34], or phenols [35], at carbon paste electrodes modified with banana, horseradish root, beet, to- [36] and zucchini [37] (utilizing the papain and ascorbic acid oxidase activities of these tissues). Simultaneous elimination of other interferences (e.g., uric acid, lipids) has been accomplished via coimmobilization of the enzymes uricase and lipase.…”

Section: Biological Modificationmentioning

confidence: 99%

Modified electrodes for electrochemical sensors

Wang

1991

Electroanalysis

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170

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Chemically modified electrodes exhibit unique behavior that can greatly benefit electrochemical sensing. In this article we review the development and applications of modified electrodes for chemical sensing. After a general introduction, different strategies for surface modification, as employed in chemical sensing, are discussed in detail. Special attention is given to permselective coatings, which offer controlled access at the sensor surface or the surface immobilization of biological entities that specifically recognize the analyte. Better understanding of these modified electrodes is achieved by using high resolution surface characterization techniques. Future prospects are evaluated. mTRODUCTIOiVThe development of electrochemical sensors continues to be a rapidly growing area of electrochemistry. Improvements in the stability, selectivity, and scope of such sensors are highly desirable to meet new challenges posed by clinical and environmental samples. The utility of solidelectrode-based sensors is often hampered by a gradual fouling of the surface due to adsorption of large organic surfactants or of reaction products. Amperometric sensors lack the ability to discriminate between solutes possessing similar redox characteristics. Finally, the sensing of many important solutes is often hindered by their slow electron transfer kinetics at the commonly used electrodes materials.One field that offers great potential for alleviating the problems above, hence for enhancing the power and scope of electrochemical sensors, is that comprising chemically modified electrodes (CMEs) [ 1, 21. The ability to deliberately control and manipulate the surface properties can lead to a variety of attractive effects. Such tailoring of the surface can meet the needs of many sensing problems. The field of CMEs, which has experienced a period of rapid growth over the past decade, has now reached a level of maturity that allows the use of these electrodes for routine sensing applications. SURFACE-MODIFID AMPEROMETHIC SENSORSThe most common approaches for incorporating a modifier into surfaces of amperometric sensors have been coverage with an appropriate polymer film and doping of a carbon paste. The modifier should be firmly attached to the surface, and it should not dissolve in the surrounding solution. The long-term stability of the attached moieties is a key factor for the performance of reliable sensors. Stability of days or weeks is essential to achieve widespread acceptance.The preparation of structure interfaces of deliberately designed character offers many promising prospects with regard to the development of chemical sensors. There are different directions by which the resulting CMEs can benefit sensing applications. These include acceleration of electron transfer reactions, prevention of fouling, permselective transport, preferential accumulation, and biocatalysis. Chemical and biological surface modifiers can thus enhance the selectivity, sensitivity, and scope of electrochemical sensors. The following sections...

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Horseradish‐root‐modified carbon paste bioelectrode

Cited by 77 publications

References 5 publications

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Application of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Rich Source of Peroxidase in the Spectrofluorometric Determination of Hydrogen Peroxide in Honey Samples

Modified electrodes for electrochemical sensors

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