In 2021, a field experiment was conducted out at ICAR-Krishi Vigyana Kendra in Bengaluru Rural District, Karnataka state, India, to investigate the impact of bio-stimulants on the growth, yield, and quality of chillies (Capsicum annuum L.). The company supplied Lakshmi Hybrid Inputs Seeds Pvt. Ltd. with Chilli Hybrid seeds (LHC-1835), that were utilized to produce the seedlings. In the present investigation, the application of amino acid-based biostimulants was carried out employing a randomized complete block design with seven treatments being replicated three times and spaced 90 by 45 cm apart. The results indicated that the maximum plant height (87.80 cm), primary branches (9.13), secondary branches (18.27), root length (27.07 cm), number of fruits per plant (58.33), fruit length (16.82 cm), and fruit diameter (1.31) with less incidence of pests such as white fly, thrips and murda complex were recorded with seedling dip and foliar application of amino acid-based bio-stimulant.