Host defense (antimicrobial) peptides

Sun, Evelyn; Belanger, Corrie R.; Haney, Evan F.; Hancock, Robert E. W.

doi:10.1016/b978-0-08-100736-5.00010-7

Cited by 45 publications

(34 citation statements)

References 158 publications

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“…In this context, since AMPs have a wide spectrum of activity and do not interact with specific receptors, it is rare to observe resistance phenotypes ( Sun et al, 2018 ). However, some cases have already been documented, such as the resistance to dermicidin developed by Staphylococcus aureus , and the reduction of sensitivity to cationic AMPs developed by some bacteria ( Ageitos et al, 2017 ).…”

Section: Introduction To Antimicrobial Peptides and Their Importancementioning

confidence: 99%

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

et al. 2018

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Antimicrobial peptides are sequences of amino acids, which present activity against microorganisms. These peptides were discovered over 70 years ago, and are abundant in nature from soil bacteria, insects, amphibians to mammals and plants. They vary in amino acids number, the distance between amino acids within individual peptide structure, net charge, solubility and other physical chemical properties as well as differ in mechanism of action. These peptides may provide an alternative treatment to conventional antibiotics, which encounter resistance such as the peptide nisin applied in treating methicillin resistant Staphylococcus aureus (MRSA) or may behave synergistically with known antibiotics against parasites for instance, nisin Z when used in synergy with ampicillin reported better activity against Pseudomonas fluorescens than when the antibiotic was alone. AMPs are known to be active against viruses, bacteria, fungi and protozoans. Nanotechnology is an arena which explores the synthesis, characterization and application of an array of delivery systems at a one billionth of meter scale. Such systems are implemented to deliver drugs, proteins, vaccines, and peptides. The role of nanotechnology in delivering AMPs is still at its early development stage. There are challenges of incorporating AMPs into drug delivery system. This review intends to explore in depth, the role of nanotechnology in delivering AMPs as well as presenting the current advances and accompanying challenges of the technology.

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Section: Introduction To Antimicrobial Peptides and Their Importancementioning

confidence: 99%

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

et al. 2018

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“…Increased bacterial resistance to conventional antibiotics requires the development of new therapeutic agents. Antimicrobial peptides, compounds that can combat infections and have an advantage over commonly used antibiotics, are regarded as promising molecules to effectively kill microorganisms [ 27 , 28 ]. During recent decades, a range of strategies have been developed to design new peptides [ 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides

Grafskaia

Pavlova

Babenko

et al. 2020

IJMS

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Antimicrobial peptides (AMPs) are considered a promising new class of anti-infectious agents. This study reports new antimicrobial peptides derived from the Hirudo medicinalis microbiome identified by a computational analysis method applied to the H. medicinalis metagenome. The identified AMPs possess a strong antimicrobial activity against Gram-positive and Gram-negative bacteria (MIC range: 5.3 to 22.4 μM), including Staphylococcus haemolyticus, an opportunistic coagulase–negative pathogen. The secondary structure analysis of peptides via CD spectroscopy showed that all the AMPs except pept_352 have mostly disordered structures that do not change under different conditions. For peptide pept_352, the α–helical content increases in the membrane environment. The examination of the mechanism of action of peptides suggests that peptide pept_352 exhibits a direct membranolytic activity. Furthermore, the cytotoxicity assay demonstrated that the nontoxic peptide pept_1545 is a promising candidate for drug development. Overall, the analysis method implemented in the study may serve as an effective tool for the identification of new AMPs.

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“…Alternatively, phage DNA can be electroporated into host cells (typical for filamentous phage-based systems; not shown). Exploiting bacterial transcription and translation mechanisms, progeny virions displaying foreign peptides are amplified and assembled (2), and released into growth medium (3). Amplified library is isolated and purified, and contacted with an immobilized target (4).…”

Section: Cellular Approachmentioning

confidence: 99%

“…The definition is rather vague in terms of chain length, although an arbitrary upper limit of 6000 Da has been assigned to label these molecules peptides, and polymers above that molecular mass are considered proteins [1]. They are ubiquitous in nature and have a role in most physiological processes as host defense (antimicrobial) agents [2], (neuro)hormones [3,4], and toxins [5]. Peptide research has experienced considerable development in the last decades, and over 7000 peptides have been identified in nature [6].…”

Section: Introductionmentioning

confidence: 99%

Evolving a Peptide: Library Platforms and Diversification Strategies

Bozovičar

Bratkovič

2019

IJMS

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Peptides are widely used in pharmaceutical industry as active pharmaceutical ingredients, versatile tools in drug discovery, and for drug delivery. They find themselves at the crossroads of small molecules and proteins, possessing favorable tissue penetration and the capability to engage into specific and high-affinity interactions with endogenous receptors. One of the commonly employed approaches in peptide discovery and design is to screen combinatorial libraries, comprising a myriad of peptide variants of either chemical or biological origin. In this review, we focus mainly on recombinant peptide libraries, discussing different platforms for their display or expression, and various diversification strategies for library design. We take a look at well-established technologies as well as new developments and future directions.

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Host defense (antimicrobial) peptides

Cited by 45 publications

References 158 publications

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides

Evolving a Peptide: Library Platforms and Diversification Strategies

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