Host Range, Purification, and Genetic Variability in <i>Sweet potato chlorotic fleck virus</i>

Aritua, V.; Barg, E.; Adipala, E.; Gibson, R. W.; Lesemann, D.‐E.; Vetten, H. J.

doi:10.1094/pdis-93-1-0087

Cited by 16 publications

(18 citation statements)

References 21 publications

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“…In addition, this might be in part explained by the fact that the tubers from the same origin are disseminated for multiplication during orchid production, resulting in the high degree of CP similarities. Based on the determination of only CP sequences of virus isolates and various analyses of the determined sequences observed from a few plant viruses, some studies showed no geographical variation of the analyzed viruses [25][26][27][28][29]. These studies support the validity of our data that the analysis of CP gene sequences is sufficient for investigating the geographical variation of CymMV isolates.…”

Section: Discussionsupporting

confidence: 84%

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

et al. 2011

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The variability in the nucleotide (nt) and amino acid (aa) sequences of the coat protein (CP) of Cymbidium mosaic virus (CymMV), which naturally infects orchids worldwide, was investigated. The CP genes of 55 CymMV isolates originating from different locations in Korea were amplified using RT-PCR and sequenced. The encoded CP consists of 223 aa. The CP sequences of the Korean isolates were compared with those of previously published CymMV isolates originating from different countries at both nt and aa levels. The Korean isolates shared 74.9-98.3 and 52.7-100% CP homology with CymMV isolates from other countries at the nt and aa levels, respectively. No particular region of variability could be found in either grouping of viruses. In the deduced CymMV CP aa sequence, the C-terminal region was more divergent than the N-terminal. The phylogenetic tree analysis based on nt sequence diversity of CP genes of CymMV isolates supported the hypothesis that CymMV isolates were divided into two subgroups. However, these subgroups were not formed by phylogenetic tree analysis of CP aa sequences. There was no distinct correlation between geographical locations and specific sequence identity, while recombination analysis revealed that there were no intra-specific recombination events among CymMV isolates.

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Section: Discussionsupporting

confidence: 84%

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

et al. 2011

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“…SPMMV is geographically restricted to East Africa [23, 71, 121], in contrast to SPCFV, which is found on many continents. Preliminary evidence suggests that SPCFV isolates from East Africa may be distinguished from those occurring elsewhere by phylogenetic analysis of CP sequences [54]. However, the inclusion of additional SPCFV isolates from East Africa and analysis of CP and NaBP sequences in this study clearly showed that SPCFV isolates from East Africa form a unique phylogenetic group.…”

Section: Discussionmentioning

confidence: 63%

“…purpurea and I . wightii were shown to be infectible with SPCFV following experimental inoculation [54], but this study showed that these species can be naturally infected with SPCFV. Furthermore, SPCSV was found to infect 12 species in the field, including H .…”

Section: Discussionmentioning

confidence: 99%

“…Similar but milder symptoms develop in sweetpotato plants co-infected with SPCSV and Sweet potato chlorotic fleck virus (SPCFV; genus Carlavirus , family Betaflexiviridae), Sweet potato mild mottle virus (SPMMV; genus Ipomovirus ; family Potyviridae) [49, 51] or sweepoviruses (genus Begomovirus , family Geminiviridae) [52]. The frequent co-infection of sweetpotatoes with SPCFV and SPFMV suggests that these viruses may be transmitted by a common vector [53, 54], but the vector of SPCFV remains to be identified [55]. Whiteflies transmit sweepoviruses and were also initially reported as vectors of SPMMV [56], but these results could not be confirmed in later studies [55].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, 12 wild Convolvulaceae species were found to be infected with SPCSV [24], but the geographical distribution of SPCSV in wild vegetation in Uganda and the wild host species and co-infection of SPCSV with other viruses in wild plants were not reported. Similarly, information regarding SPCFV infection in wild plants of Convolulacea is lacking, even though SPCFV occurs sweetpotatoes in Uganda [58] and other East African countries such as Kenya [53, 59], Tanzania [60] and Rwanda [61], as well as western Africa [62], Asia, Australia, East Timor and Latin America [54, 63–66]. …”

Section: Introductionmentioning

confidence: 99%

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Mixed Infections of Four Viruses, the Incidence and Phylogenetic Relationships of Sweet Potato Chlorotic Fleck Virus (Betaflexiviridae) Isolates in Wild Species and Sweetpotatoes in Uganda and Evidence of Distinct Isolates in East Africa

2016

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Viruses infecting wild flora may have a significant negative impact on nearby crops, and vice-versa. Only limited information is available on wild species able to host economically important viruses that infect sweetpotatoes (Ipomoea batatas). In this study, Sweet potato chlorotic fleck virus (SPCFV; Carlavirus, Betaflexiviridae) and Sweet potato chlorotic stunt virus (SPCSV; Crinivirus, Closteroviridae) were surveyed in wild plants of family Convolvulaceae (genera Astripomoea, Ipomoea, Hewittia and Lepistemon) in Uganda. Plants belonging to 26 wild species, including annuals, biannuals and perennials from four agro-ecological zones, were observed for virus-like symptoms in 2004 and 2007 and sampled for virus testing. SPCFV was detected in 84 (2.9%) of 2864 plants tested from 17 species. SPCSV was detected in 66 (5.4%) of the 1224 plants from 12 species sampled in 2007. Some SPCSV-infected plants were also infected with Sweet potato feathery mottle virus (SPFMV; Potyvirus, Potyviridae; 1.3%), Sweet potato mild mottle virus (SPMMV; Ipomovirus, Potyviridae; 0.5%) or both (0.4%), but none of these three viruses were detected in SPCFV-infected plants. Co-infection of SPFMV with SPMMV was detected in 1.2% of plants sampled. Virus-like symptoms were observed in 367 wild plants (12.8%), of which 42 plants (11.4%) were negative for the viruses tested. Almost all (92.4%) the 419 sweetpotato plants sampled from fields close to the tested wild plants displayed virus-like symptoms, and 87.1% were infected with one or more of the four viruses. Phylogenetic and evolutionary analyses of the 3′-proximal genomic region of SPCFV, including the silencing suppressor (NaBP)- and coat protein (CP)-coding regions implicated strong purifying selection on the CP and NaBP, and that the SPCFV strains from East Africa are distinguishable from those from other continents. However, the strains from wild species and sweetpotato were indistinguishable, suggesting reciprocal movement of SPCFV between wild and cultivated Convolvulaceae plants in the field.

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Ipomoea batatas (Sweet potato)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Host Range, Purification, and Genetic Variability in Sweet potato chlorotic fleck virus

Cited by 16 publications

References 21 publications

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

Mixed Infections of Four Viruses, the Incidence and Phylogenetic Relationships of Sweet Potato Chlorotic Fleck Virus (Betaflexiviridae) Isolates in Wild Species and Sweetpotatoes in Uganda and Evidence of Distinct Isolates in East Africa

Ipomoea batatas (Sweet potato)

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