Host Range Restriction of Insect-Specific Flaviviruses Occurs at Several Levels of the Viral Life Cycle

Junglen, Sandra; Korries, Marvin; Grasse, Wolfgang; Wieseler, Janett; Kopp, Anne; Hermanns, Kyra; León-Juárez, Moisés; Drosten, Christian; Kümmerer, Beate M.

doi:10.1128/msphere.00375-16

Cited by 65 publications

(60 citation statements)

References 49 publications

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“…Each of these steps is an obstacle to be overcome by the virus [ 32 ]. To study the mechanisms behind host-range restrictions reverse genetic systems have proven to be a valuable tool through the ability of creating chimeric viruses [ 28 , 31 , 33 ].…”

Section: Host-range Restrictions Of Isvsmentioning

confidence: 99%

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Insect-specific virus evolution and potential effects on vector competence

2019

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The advancement in high-throughput sequencing technology and bioinformatics tools has spurred a new age of viral discovery. Arthropods is the largest group of animals and has shown to be a major reservoir of different viruses, including a group known as insect-specific viruses (ISVs). The majority of known ISVs have been isolated from mosquitoes and shown to belong to viral families associated with animal arbovirus pathogens, such as Flaviviridae, Togaviridae and Phenuiviridae . These insect-specific viruses have a strict tropism and are unable to replicate in vertebrate cells, these properties are interesting for many reasons. One is that these viruses could potentially be utilised as biocontrol agents using a similar strategy as for Wolbachia . Mosquitoes infected with the viral agent could have inferior vectorial capacity of arboviruses resulting in a decrease of circulating arboviruses of public health importance. Moreover, insect-specific viruses are thought to be ancestral to arboviruses and could be used to study the evolution of the switch from single-host to dual-host. In this review, we discuss new discoveries and hypothesis in the field of arboviruses and insect-specific viruses.

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Section: Host-range Restrictions Of Isvsmentioning

confidence: 99%

“…A study by Junglen et al [ 33 ] also showed that the host-range restriction can be at several levels of the viral life cycle. In this study chimera Yellow fever virus carrying envelope proteins from the insect-specific flavivirus Niénokoué virus (NIEV) was use to study host range restrictions.…”

Section: Host-range Restrictions Of Isvsmentioning

confidence: 99%

Insect-specific virus evolution and potential effects on vector competence

2019

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“…WNV mutants lacking production of sfRNA1, or sfRNA1 and sfRNA2 were shown to exhibit reduced pathogenicity in mice (Pijlman et al, 2008). Later studies demonstrated the pivotal role of sfRNA in replication, dissemination and transmission of a wide range of flaviviruses (Chang et al, 2013;Donald et al, 2016;Filomatori et al, 2017;Junglen et al, 2017).…”

Section: Functions Of Sfrnamentioning

confidence: 99%

Subgenomic flaviviral RNAs: What do we know after the first decade of research

2018

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The common feature of flaviviral infection is the accumulation of abundant virus-derived noncoding RNA, named flaviviral subgenomic RNA (sfRNA) in infected cells. This RNA represents a product of incomplete degradation of viral genomic RNA by the cellular 5'-3' exoribonuclease XRN1 that stalls at the conserved highly structured elements in the 3' untranslated region (UTR). This mechanism of sfRNA generation was discovered a decade ago and since then sfRNA has been a focus of intense research. The ability of flaviviruses to produce sfRNA was shown to be evolutionary conserved in all members of Flavivirus genus. Mutations in the 3'UTR that affect production of sfRNAs and their interactions with host factors showed that sfRNAs are responsible for viral pathogenicity, host adaptation, and emergence of new pathogenic strains. RNA structural elements required for XRN1 stalling have been elucidated and the role of sfRNAs in inhibiting host antiviral responses in arthropod and vertebrate hosts has been demonstrated. Some molecular mechanisms determining these properties of sfRNA have been recently characterized, while other aspects of sfRNA functions remain an open avenue for future research. In this review we summarise the current state of knowledge on the mechanisms of generation and functional roles of sfRNAs in the life cycle of flaviviruses and highlight the gaps in our knowledge to be addressed in the future.

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“…All together, the current studies highlight gaps in the understanding of interactions between ISVs and other pathogenic viruses. It has been shown that the host range restriction of insect-specific flaviviruses and alphaviruses occurs at different levels of viral replication cycle (Nasar et al, 2015;Junglen et al, 2017). Among intrinsic and extrinsic factors affecting viral replication and transmissibility, temperature was shown to be of an extreme importance (Samuel et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Dianke virus: A new mesonivirus species isolated from mosquitoes in Eastern Senegal

et al. 2020

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An increasing number of insect-specific viruses are found around the world. Very recently, a new group of insectspecific viruses, the Mesoniviridae family, was discovered in Africa, Asia, North America and Australia. Here we report the first detection and isolation of a new virus belonging to Mesonivirus genus in Senegal, West Africa. The so-called Dianke virus was detected in 21 species of arthropods trapped in the eastern part of the country. Male individuals were also infected, supporting vertical transmission assertion of insect specific viruses. As described for other mesoniviruses, no viral replication was observed after inoculation of mammalian cells. Viral replication in mosquito cells was blocked at a temperature of 37°C, highlighting the importance of thermal conditions in Mesonivirus host restriction. Similar to our study, where a diverse range of arthropod vectors were found infected by the new virus, several studies have detected mesonivirus infection in mosquitoes with concerns for human health. It has been shown that dual infections in mosquito can alter viral infectivity. Due to their extensive geographic distribution and host range, as well as their use as potential disease control agents in vector populations, more studies should be done for a better knowledge of arthropod-restricted viruses prevalence and diversity.

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Host Range Restriction of Insect-Specific Flaviviruses Occurs at Several Levels of the Viral Life Cycle

Cited by 65 publications

References 49 publications

Insect-specific virus evolution and potential effects on vector competence

Insect-specific virus evolution and potential effects on vector competence

Subgenomic flaviviral RNAs: What do we know after the first decade of research

Dianke virus: A new mesonivirus species isolated from mosquitoes in Eastern Senegal

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