Host‐specific effect of P1 exchange between two potyviruses

Abstract

The potyviruses Plum pox virus (PPV) and Tobacco vein mottling virus (TVMV) have distinct host ranges and induce different symptoms in their common herbaceous hosts. To test the relevance of the P1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the P1 coding sequence of PPV was completely or partially replaced by the corresponding sequences from TVMV. Infections induced by these chimeric viruses revealed that the TVMV P1 and a PPV/TVMV hybrid P1 proteins are functionally equivalent in herbaceous plants to the P1 protein of a PPV isolate adapted to these hosts, in spite of having high sequence divergence. Moreover, the presence of TVMV P1 sequences enhanced the competence of a low-infectivity PPV-D-derived chimera in Nicotiana clevelandii. Conversely, all PPV/TVMV hybrids were unable to infect Prunus persicae, a specific host for PPV, suggesting that TVMV P1 is not functionally competent in this plant. Together, these data highlight the importance of the P1 protein in defining the virus host range.

Recombination and selection pressure in the ipomovirus sweet potato mild mottle virus (Potyviridae) in wild species and cultivated sweetpotato in the centre of evolution in East Africa

“…In comparison to a number of viruses in the family Potyviridae studied previously (reviewed by García-Arenal et al, 2001), SPMMV exhibited relatively high nucleotide diversity in the P1 region (Adams et al, 2005b). Data of previous studies suggest that P1 may be crucial for host adaptation in potyviruses (Shi et al, 2007;Salvador et al, 2008a). P1 proteins of potyviruses such as turnip mosaic virus and potato virus Y (PVY) have the largest v values among the mature viral proteins (at the population level), (Colinet et al, 1998) and E/P (Mukasa et al, 2003b) (broken arrows) are also shown.…”

Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

“…At least 36 recombination events were found between PRSV-P India and 11 other PRSV genomes from the rest of the world. The textured boxes are recombination hotspots assessed by the recombination detection program GeneConv range determination is also reported for Plum pox virus (PPV) and Tobacco vein mottling virus (TVMV) (Salvador et al 2008).…”

P1 peptidase – a mysterious protein of family Potyviridae

“…On the one hand, there are those trying to solve the real function of P1 protease in several potyviruses: Plum pox virus (PPV) (Salvador et al 2008), Tobacco vein mottling virus (TVMV) (Brantley and Hunt 1993;Klein et al 1994;Salvador et al 2008), Cucumber vein yellowing virus (CVYV) (Valli et al 2006(Valli et al , 2008, Soybean mosaic virus (SMV) (Gagarinova et al…”