“Host Tissue Damage” Signal ATP Promotes Non-directional Migration and Negatively Regulates Toll-like Receptor Signaling in Human Monocytes

Kaufmann, Andreas M.; Musset, Boris; Limberg, Sven H.; Renigunta, Vijay; Sus, Rainer; Dalpke, Alexander H.; Heeg, Klaus; Robaye, Bernard; Hanley, Peter J.

doi:10.1074/jbc.m505301200

Cited by 80 publications

(67 citation statements)

References 47 publications

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“…Of the seven P2X receptors (P2X 1 , P2X 2 , P2X 3 , P2X 4 , P2X 5 , P2X 6 , and P2X 7 ) currently known, human monocytes express P2X 1 , P2X 4 , and P2X 7 receptors as revealed at the mRNA level by reverse transcription PCR analysis [24]. Measuring surface expression of the receptor proteins by FACS analysis revealed expression of both P2X 1 (Fig.…”

Section: Resultsmentioning

confidence: 93%

Involvement of P2X receptors in the NAD+-induced rise in [Ca2+]i in human monocytes

Grahnert

Klein

Hauschildt

2009

Purinergic Signalling

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In the present study, we show that the extracellular addition of nicotinamide adenine dinucleotide (NAD + ) induces a transient rise in [Ca 2+ ] i in human monocytes caused by an influx of extracellular calcium. The NAD + -induced Ca 2+ response was prevented by adenosine triphosphate (ATP), suggesting the involvement of ATP receptors. Of the two subtypes of ATP receptors (P2X and P2Y), the P2X receptors were considered the most likely candidates. By the use of subtype preferential agonists and antagonists, we identified P2X 1 , P2X 4 , and P2X 7 receptors being engaged in the NAD + -induced rise in [Ca 2+ ] i . Among the P2X receptor subtypes, the P2X 7 receptor is unique in facilitating the induction of nonselective pores that allow entry of ethidium upon stimulation with ATP. In monocytes, opening of P2X 7 receptor-dependent pores strongly depends on specific ionic conditions. Measuring pore formation in response to NAD + , we found that NAD + unlike ATP lacks the ability to induce this pore-forming response. Whereas as little as 100 μM ATP was sufficient to activate the nonselective pore, NAD + at concentrations up to 2 mM had no effect. Taken together, these data indicate that despite similarities in the action of extracellular NAD + and ATP there are nucleotide-specific variations. So far, common and distinct features of the two nucleotides are only beginning to be understood.

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Section: Resultsmentioning

confidence: 93%

Involvement of P2X receptors in the NAD+-induced rise in [Ca2+]i in human monocytes

Grahnert

Klein

Hauschildt

2009

Purinergic Signalling

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“…Thus, injury-induced release of ATP and its subsequent induction of Ca 2+ waves may serve as an early response to stress including wounding and/or infection (Kaufmann et al, 2005), and provide a tissue damage signal to promote wound healing and functional recovery in epithelial cells. Because ATP and other P2Y-receptor agonists, such as diuridine tetraphosphate (diquafosol) and the uracil-cytosine dinucleotide denufosol, are already in clinical trials for certain diseases, including dry-eye disease, retinal detachment, upper respiratory tract symptoms, cystic fibrosis and lung cancer (Agteresch et al, 2000;Agteresch et al, 2002;Brunschweiger and Muller, 2006), these findings may eventually lead to applications in preventing and/or treating wound-healingrelated diseases in the cornea and other tissues and organs.…”

Section: Fig 7 Atp-␥-s-induced Hb-egf Shedding and Egfr Activation mentioning

confidence: 99%

Wound-induced ATP release and EGF receptor activation in epithelial cells

Yin

Zhang

et al. 2007

Journal of Cell Science

158

173

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“…185,186 Hypoxia and ischemia cause the release of intracellular substances (ie, organelles, heat shock proteins, ATPγ [*PLS. SPELL OUT*], etc) that may play a role in promoting inflammation.…”

Section: Reduction Of Local Hypoxia/ Cross-tolerancementioning

confidence: 99%

“…SPELL OUT*], etc) that may play a role in promoting inflammation. 185,186 Ischemia and hypoxia are physiological responses associated with both exercise-induced damage to skeletal muscle and adipocytes.Flynn et al …”

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confidence: 99%

State of the Art Reviews: The Anti-Inflammatory Actions of Exercise Training

Flynn

McFarlin

Markofski³

2007

American Journal of Lifestyle Medicine

111

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The list of diseases with a known inflammatory etiology is growing. Cardiovascular disease, osteoporosis, diabetes, geriatric cachexia, and Alzheimer's disease have all been shown to be linked to or exacerbated by aberrantly regulated inflammatory processes. Nevertheless, there is mounting evidence that those who are physically active, or who become physically active, have a reduction in biomarkers associated with chronic inflammation. There was strong early consensus that exercise-induced reductions in inflammation were explained by body mass index or body fatness, but recent studies provide support for the contention that exercise has body fatindependent anti-inflammatory effects. With few exceptions, the anti-inflammatory effects of exercise appear to occur regardless of age or the presence of chronic diseases. What remains unclear are the mechanisms by which exercise training induces these anti-inflammatory effects, but there are several intriguing possibilities, including release of endogenous products, such as heat shock proteins; selective reduction of visceral adipose tissue mass or reducing infiltration of adipocytes by macrophages; shift in immune cell phenotype; cross-tolerizing effects; or exerciseinduced shifts in accessory proteins of toll-like receptor signaling. However, future research endeavors are likely to uncover additional potential mechanisms, and it could be some time before functional mechanisms are made clear. In summary, the potential anti-inflammatory influences of exercise training may provide a low-cost, readily available, and effective treatment for low-grade systemic inflammation and could contribute significantly to the positive effects of exercise training on chronic disease. Keywordsanti-inflammatory; exercise training; chronic disease Cardiovascular disease, diabetes, osteoporosis, and several other chronic diseases are now known to be strongly linked to inflammatory processes. [1][2][3][4] Chronic diseases linked to inflammation are known to occur in greater frequency in older and sedentary individuals, 5 but evidence is emerging that exercise has anti-inflammatory effects. [6][7][8][9] For example, there is a substantial reduction in biomarkers of low-grade systemic inflammation in physically active adults or adults who undertake exercise training. [10][11][12][13][14] Address for correspondence: Michael G. Flynn, FACSM, Wastl Human Performance Lab, Department of Health and Kinesiology, Purdue University West Lafayette, IN 47907; mickflyn@purdue.edu. NIH Public Access Author ManuscriptAm J Lifestyle Med. Author manuscript; available in PMC 2014 November 25. NIH-PA Author ManuscriptChronic low-grade inflammation, "persistent but more subtle than the acute phase response," 15 is frequently assessed by measurement of circulating C-reactive protein (CRP) and, somewhat less frequently, serum amyloid A. C-reactive protein concentration is higher in those with high body mass index, 16,17 metabolic syndrome and/or diabetes, 1,18 belownormal high-density lipoprotein choleste...

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“Host Tissue Damage” Signal ATP Promotes Non-directional Migration and Negatively Regulates Toll-like Receptor Signaling in Human Monocytes

Cited by 80 publications

References 47 publications

Involvement of P2X receptors in the NAD+-induced rise in [Ca2+]i in human monocytes

Involvement of P2X receptors in the NAD+-induced rise in [Ca2+]i in human monocytes

Wound-induced ATP release and EGF receptor activation in epithelial cells

State of the Art Reviews: The Anti-Inflammatory Actions of Exercise Training

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