Hotair and Malat1 Long Noncoding RNAs regulate Bdnf Expression and Oligodendrocyte Precursor Cells Differentiation

Abstract

BDNF has remarkable protective roles in the central nervous system to ensure neurons and glial cell survival and proper functions. The regulatory processes behind the BDNF expression have not been revealed completely. Here, it was explored whether Malat1 and Hotair lncRNAs play roles in the regulation of Bdnf expression level, modification of fingolimod downstream pathway, and oligodendrocytes precursor cells maturation. By Hotair and Malat1 downregulation, their regulatory mechanism on Bdnf expression was investigated. Immunostaining and RT-qPCR assays were employed to assess the effects of fingolimod and lncRNAs on OPCs maturation. The results represented that Hotair and Malat1 lncRNAs may regulate Bdnf expression in primary glial cells significantly, and also can coordinate fingolimod stimulatory effect on Bdnf expression. Furthermore, Malat1 may have a role in the last stages of the intrinsic oligodendrocyte myelination. Here it was demonstrated that these lncRNAs have critical roles in the Bdnf level, fingolimod mechanism of action, and OPCs maturation. Understanding the regulatory mechanism of neurotrophins leads to a better comprehension of the pathogenesis of the neurodegenerative disorder and designing more effective treatments.

The kappa opioid receptor agonist, nalfurafine, and its mechanism of action in promoting remyelination

“…Zhou et al, 2021). Malat1 can modulate immune responses (Hewitson et al, 2020;Wu et al, 2018) and is involved in myelination (Khani-Habibabadi et al, 2022;Scherer et al, 2020).…”