HOTAIR in Relation to Epithelial-Mesenchymal Transition and Cancer Stem Cells in Molecular Subtypes of Endometrial Cancer

Łuczak, Agata; Supernat, Anna; Łapińska‐Szumczyk, Sylwia; Jachimowicz, Daniel; Majewska, Hanna; Gulczyński, Jacek; Żaczek, Anna

doi:10.5301/jbm.5000187

Cited by 23 publications

(18 citation statements)

References 23 publications

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“…Simultaneously, HOTAIR could facilitate transformation of gastric cancer tissues from polarized epithelial cells to mobile mesenchymal cells, and also trigger abnormal expressions of genes relevant to EMT and cancer metastasis, including ICAM‐1, MMP1, MMP3, MMP9, and Snail . Correspondingly, knockout of HOTAIR could induce hindered EMT process, which could be reversed by addition of exogenous Snail . Hence, it was well‐explained that HOTAIR might facilitate the progression of gastric cancer through mediation of the EMT process in this study (Figure ).…”

Section: Discussionmentioning

confidence: 53%

The contrary functions of lncRNA HOTAIR/miR‐17‐5p/PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells

Jia

Zhan

et al. 2018

J Cellular Molecular Medi

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This study was implemented to figure out whether lncRNA HOTAIR/miR‐17‐5p/PTEN axis played a role that was opposite to Shenqifuzheng (SQFZ) injection in regulating the chemosensitivity of gastric cancer cells. The gastric cancer tissues were gathered and four gastric cancer cell lines were prepared, including BGC‐823, MGC‐803, SGC‐7901, and MKN28. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluoroura were managed as the chemo‐therapeutics, and SQFZ was prepared as a Chinese medicine. Striking distinctions of HOTAIR, miR‐17‐5p, and PTEN expressions were observed between gastric cancer tissues and para‐carcinoma normal tissues (P < 0.05). MKN28 was associated with the highest resistance to cisplatin, adriamycin, mitomycin, and 5‐fluoroura among all the cell types, and SQFZ significantly improved the MKN28 cells’ sensitivity to the drugs (P < 0.05). The over‐expressed HOTAIR and miR‐17‐5p, as well as under‐expressed PTEN tended to significantly facilitate the viability, EMT process and proliferation of MKN28 cells that were subject to treatment of chemo‐therapies (P < 0.05). SQFZ could amplify the effects of si‐HOTAIR, miR‐17‐5p inhibitor, and pcDNA‐PTEN on boosting the chemosensitivity of gastric cancer cells (P < 0.05). In addition, HOTAIR was also found to directly target miR‐17‐5p, and PTEN appeared to be subject to the modification of HOTAIR and miR‐17‐5p in its acting on the viability, proliferation, EMT process, and apoptosis of gastric cancer cells. The HOTAIR/miR‐17‐5p/PTEN axis could be regarded as the potential treatment targets for gastric cancer, and adjuvant therapy of SQFZ injection could assist in further improving the treatment efficacy of chemo‐therapies for gastric cancer.

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Section: Discussionmentioning

confidence: 53%

The contrary functions of lncRNA HOTAIR/miR‐17‐5p/PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells

Jia

Zhan

et al. 2018

J Cellular Molecular Medi

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“…For instance, long noncoding RNA HOX antisense intergenic RNA (HOTAIR), closely associated with metastasis, epithelial–mesenchymal transition (EMT), and poor prognosis, has emerged as a key metastatic molecule in many cancers [8–10]. The metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), the first lncRNA identified as an independent prognostic marker for nonsmall-cell lung cancer [11], was found to play an important role in the metastasis of various types of cancers [12–14].…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO

Yang

Chen

et al. 2016

Oncotarget

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This study aimed to investigate the role of long noncoding RNAs (lncRNAs) in the metastasis of colorectal cancer (CRC). Metastasis is an important prognostic factor of CRC, and lncRNAs have been implicated in tumor proliferation and metastasis. The human CRC cell lines HCT116, HT29, SW480, DLD-1, and SW620 were used in the study. Genome-wide lncRNA expression patterns in metastatic lymph nodes compared with paired normal lymph nodes of CRC were assessed by microarray analysis. Gastric adenocarcinoma predictive long intergenic noncoding (GAPLINC) RNA was detected via functional prediction. The increased expression of GAPLINC was found to be positively correlated with larger tumor size, advanced tumor stage (T stage), advanced node stage (N stage), increased death, and shorter survival of patients with CRC by in situ hybridization analysis. Besides, the decreased expression of GAPLINC could significantly repress CRC cell invasion in vitro and also inhibit proliferation in vitro and in vivo. RNA pull-down with mass spectrum experiments revealed that PTB-associated splicing factor (PSF) and non-POU-domain-containing octamer-binding (NONO) protein bound to GAPLINC and reversed the effect of GAPLINC on cell invasion. Gene array and bioinformatics analyses identified that snail family zinc finger 2 (SNAI2) was involved in the biological processes of GAPLINC/PSF/NONO. This study indicated the importance of GAPLINC in promoting CRC invasion via binding to PSF/NONO and partly by stimulating the expression of SNAI2. Hence, GAPLINC may serve as a promising target for CRC diagnosis and therapy. The findings may help in developing a novel therapeutic strategy for patients with CRC.

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“…[85][86][87][88][89] HOTAIR expression supports metastasis in breast cancer and has been linked to poor outcome in several malignancies. 85 Padua Alves et al 86 examined breast and colon cancer cell lines in studies of HOTAIR regulation and found that this lncRNA was increased after TGFβ1 treatment in both breast and colon cancers.…”

Section: Hox Transcript Antisense Intergenic Rna (Hotair)mentioning

confidence: 99%

“…86 HOTAIR has also been implicated in the regulation of CSCs in cervical cancer and endometrial cancer. [87][88][89] LINC-ROR Linc-ROR was first discovered in induced pluripotent stem cells (iPSCs) where it was regulated by the key pluripotency factors Oct4, Sox2 and Nanog. 90 Linc-ROR has a principal role in maintenance of iPSCs and embryonic stem cells by preventing the activation of cellular stress pathways including the p53 response.…”

Section: Hox Transcript Antisense Intergenic Rna (Hotair)mentioning

confidence: 99%

The evolving concept of cancer stem-like cells in thyroid cancer and other solid tumors

Hardin

Zhang

Helein

et al. 2017

Laboratory Investigation

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The cancer stem-like cell (CSC) hypothesis postulates that a small population of cells in a cancer has self-renewal and clonal tumor initiation properties. These cells are responsible for tumor initiation, growth, recurrence and for resistance to chemotherapy and radiation therapy. CSCs can be characterized using markers such as SSEA-1, SSEA-4, CD44, CD24, ALDEFLUOR and others. CSCs form spheres when they are cultured in serum-free condition in low attachment plates and can generate tumors when injected into immune-deficient mice. During epithelial to mesenchymal transition (EMT), cells lose cellular adhesion and polarity and acquire an invasive phenotype. Recent studies have established a relationship between EMT and increased numbers of CSCs in some solid malignancies. Non-coding RNAs such as microRNAs and long non-coding RNAs (lncRNAs) have been shown to have important roles during EMT and some of these molecules also have regulatory roles in the proliferation of CSCs. Specific lncRNAs enhanced cell migration and invasion in breast carcinomas, which was associated with the generation of stem cell properties. The tumor microenvironment of CSCs also has an important role in tumor progression. Recent studies have shown that the interaction between tumor cells and the local microenvironment at the metastatic site leads to the development of premetastatic niche(s) and allows for the proliferation of the metastatic cells during colonization. The role of exosomes in the microenvironment during the EMT program is currently a major area of research. This review examines CSCs and the relationship between EMT and CSCs in solid tumors with emphasis on thyroid CSCs. The role of non-coding RNAs and of the microenvironment in EMT and in tumor progression are also examined. This review also highlights the growing number of studies that show the close association of EMT and CSCs and the role of exosomes and other elements of the tissue microenvironment in CSC metastasis. A better understanding of these mechanisms will lead to more effective targeting of primary and metastatic malignancies.

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HOTAIR in Relation to Epithelial-Mesenchymal Transition and Cancer Stem Cells in Molecular Subtypes of Endometrial Cancer

Cited by 23 publications

References 23 publications

The contrary functions of lncRNA HOTAIR/miR‐17‐5p/PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells

The contrary functions of lncRNA HOTAIR/miR‐17‐5p/PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells

Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO

The evolving concept of cancer stem-like cells in thyroid cancer and other solid tumors

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