How abundant are superoxide and hydrogen peroxide in the vasculature lumen, how far can they reach?

Sousa, Teresa; Gouveia, Marcos; Travasso, Rui D. M.; Salvador, Armindo

doi:10.1016/j.redox.2022.102527

Cited by 12 publications

(10 citation statements)

References 81 publications

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“…Second, H 2 O 2 is known to be able to diffuse in the extracellular space, transmitting oxidative cell injury. However, the distance of diffusion is limited [35][36][37]. The oxidized serum proteins could serve as an even more critical mediator transmitting the oxidative injury from the local source of H 2 O 2 to the remote organs, such as kidney.…”

Section: Discussionmentioning

confidence: 99%

The Combined Administration of Vitamin C and Copper Induces a Systemic Oxidative Stress and Kidney Injury

et al. 2023

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Vitamin C (ascorbic acid; AA) and copper (Cu2+) are well used supplements with many health-promoting actions. However, when they are used in combination, the Fenton reaction occurs, leading to the formation of highly reactive hydroxyl radicals. Given that kidney is vulnerable to many toxicants including free radicals, we speculated that the in vivo administration of AA plus Cu2+ may cause oxidative kidney injury. The purpose of this study was to address this possibility. Mice were administered with AA and Cu2+, alone or in combination, via oral gavage once a day for various periods. Changes in the systemic oxidative status, as well renal structure and functions, were examined. The administration of AA plus Cu2+ elevated protein oxidation in serum, intestine, bladder, and kidney, as evidenced by the increased sulfenic acid formation and decreased level of free sulfhydryl groups (-SH). The systemic oxidative stress induced by AA plus Cu2+ was associated with a significant loss of renal function and structure, as indicated by the increased blood urea nitrogen (BUN), creatinine and urinary proteins, as well as glomerular and tubular cell injury. These effects of AA and Cu2+ were only observed when used in combination, and could be entirely prevented by thiol antioxidant NAC. Further analysis using cultured renal tubular epithelial cells revealed that AA plus Cu2+ caused cellular protein oxidation and cell death, which could be abolished by NAC and catalase. Moreover, coincubation of AA and Cu2+ led to H2O2 production. Collectively, our study revealed that a combined administration of AA and Cu2+ resulted in systemic oxidative stress and renal cell injury. As health-promoting supplements, AA and Cu2+ should not be used together.

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Section: Discussionmentioning

confidence: 99%

The Combined Administration of Vitamin C and Copper Induces a Systemic Oxidative Stress and Kidney Injury

et al. 2023

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“…These reactive species have the potential to target erythrocytes and may enter through various channels, such as aquaporins 123 . A recent computational study found that 36% and 82% of the plasma hydrogen peroxide is absorbed by the erythrocyte in the capillaries and arterioles, respectively 189 . This finding highlights the potential impact of reactive species of plasma on erythrocyte redox metabolism.…”

Section: The Map Of Erythrocyte Metabolismmentioning

confidence: 98%

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

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Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map. The focus of this map is on the main biochemical pathways consisting of glycolysis, the pentose phosphate pathway, redox metabolism, oxygen metabolism, purine/nucleoside metabolism, and membrane transport. Other recently emerging pathways are also curated, like the methionine salvage pathway, the glyoxalase system, carnitine metabolism, and the lands cycle, as well as remnants of the carboxylic acid metabolism. An additional goal of this review is to present the dynamics of erythrocyte metabolism, providing key numbers used to perform basic quantitative analyses. By synthesizing experimental and computational data, we conclude that glycolysis, pentose phosphate pathway, and redox metabolism are the foundations of erythrocyte metabolism. Additionally, the erythrocyte can sense oxygen levels and oxidative stress adjusting its mechanics, metabolism, and function. In conclusion, fine‐tuning of erythrocyte metabolism controls one of the most important biological processes, that is, oxygen loading, transport, and delivery.

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“…Overall, unless H 2 O 2 -generating mechanisms are packed into exosomes, it is believed that the distance an extracellular H 2 O 2 signal can travel in mammalian tissues is in the range of 100–200 μm, and potentially up to 1 mm 19-21,23 . This limitation is mainly attributed to the H 2 O 2 scavenging activity of the extracellular matrix/neighboring cells, that generates a gradient from the producing cells, all the way up to the point H 2 O 2 levels drop to normal levels at the tissue(s) they are generated at [ [19] , [20] , [21] , 23 ]. Recent studies in plant and mammalian cells have, however, challenged this concept showing that H 2 O 2 signals can propagate within minutes over long distances (measured in centimeters) in different plant tissues, as well as in monolayers of human MDA-MB-231 breast cancer and rat H9c2 cardiomyocyte cells, and isolated mice hearts [ 22 , [24] , [25] , [26] , [27] , [28] , [29] ].…”

Section: Introductionmentioning

confidence: 99%

Propagation of a rapid cell-to-cell H2O2 signal over long distances in a monolayer of cardiomyocyte cells

Fichman,

Rowland,

Nguyen

et al. 2024

Redox Biology

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How abundant are superoxide and hydrogen peroxide in the vasculature lumen, how far can they reach?

Cited by 12 publications

References 81 publications

The Combined Administration of Vitamin C and Copper Induces a Systemic Oxidative Stress and Kidney Injury

The Combined Administration of Vitamin C and Copper Induces a Systemic Oxidative Stress and Kidney Injury

Erythrocyte metabolism

Propagation of a rapid cell-to-cell H2O2 signal over long distances in a monolayer of cardiomyocyte cells

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