How can environmental conditions influence dicofol genotoxicity on the edible Asiatic clam, Meretrix meretrix?

Ivorra, Lucia; Cruzeiro, Catarina; Ramos, Altina; Tagulao, Karen Araño; Cardoso, P.G.

doi:10.1016/j.envpol.2021.118467

Cited by 3 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, under a fluorescence microscope, intact DNA appears as a round or oval structure (the 'head'), while damaged DNA forms a 'tail', resembling the comet [ 84 ]. This rapid and sensitive technique requires only a small number of cells, yet it provides information regarding the DNA damage and potential repair at the individual cell level [ 85 ].…”

Section: Chemical Physical and Microbiological Characterisationmentioning

confidence: 99%

“…For each animal, four slides were prepared (for assessing possible direct DNA damages and the H 2 O 2 -induced effects, respectively. Cell lysis and electrophoresis protocol were followed as mentioned in Ivorra et al [ 85 ]. Briefly, cells were lysed by incubating the slides in a lysis solution (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris base, 1% Triton-X pH 10) for 2 h at 4 °C in the dark.…”

Section: Chemical Physical and Microbiological Characterisationmentioning

confidence: 99%

See 1 more Smart Citation

To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse

Bertanza,

Abbà,

Alias

et al. 2024

MethodsX

Self Cite

View full text Add to dashboard Cite

Section: Chemical Physical and Microbiological Characterisationmentioning

confidence: 99%

Section: Chemical Physical and Microbiological Characterisationmentioning

confidence: 99%

To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse

Bertanza,

Abbà,

Alias

et al. 2024

MethodsX

Self Cite

View full text Add to dashboard Cite

“…Dicofol (2,2,2-trichloro-1,1-bis (4-chlorophenyl) ethanol, CAS 115-32-2), as one of the major classes of organochlorine pesticides, was used on a worldwide variety of fruit, ornamentals, and field crops previously [14,15]. Despite the agricultural benefits of dicofol, numerous studies have demonstrated its toxicity, capacity for endocrine disruption, and carcinogenicity via the biological accumulation effect [16,17]. Stockholm Convention identified dicofol as a persistent organic pollutant (POP) that many countries banned or limited its use [14].…”

Section: Introductionmentioning

confidence: 99%

Spontaneous In-Source Fragmentation Reaction Mechanism and Highly Sensitive Analysis of Dicofol by Electrospray Ionization Mass Spectrometry

Xie

Guo

et al. 2023

Molecules

View full text Add to dashboard Cite

Although dicofol has been widely banned all over the world as a kind of organochlorine contaminant, it still exists in the environment. This study developed a high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) detection technique for dicofol, an environmental pollutant, for the first time using in-source fragmentation. The results confirmed that m/z 251 was the only precursor ion of dicofol after in-source fragmentation, and m/z 139 and m/z 111 were reasonable product ions. The main factors triggering the in-source fragmentation were the H+ content and solution conductivity when dicofol entered the mass spectrometer. Density functional theory can be used to analyze and interpret the mechanism of dicofol fragmentation reaction in ESI source. Dicofol reduced the molecular energy from 8.8 ± 0.05 kcal/mol to 1.0 ± 0.05 kcal/mol, indicating that the internal energy release from high to low was the key driving force of in-source fragmentation. A method based on HPLC-MS/MS was developed to analyze dicofol residues in environmental water. The LOQ was 0.1 μg/L, which was better than the previous GC or GC-MS methods. This study not only proposed an HPLC-MS/MS analysis method for dicofol for the first time but also explained the in-source fragmentation mechanism of compounds in ESI source, which has positive significance for the study of compounds with unconventional mass spectrometry behavior in the field of organic pollutant analysis and metabonomics.

show abstract

“…Comet assay is a sensitive, simple, flexible, rapid, and inexpensive technique for detecting DNA damage as DNA strand breaks in an individual cell of almost any eukaryotic organism (Azqueta & Collins, 2013;Cvetković et al, 2018;Tatin et al, 2021). Due to its ability and practical advantages, this assay has already been used as a genotoxicity test for various genotoxicants both direct and indirect-acting (Erraud et al, 2018;González et al, 2018;Gajski et al, 2019;Cordelli et al, 2021;Rajendran et al, 2021) for various aquatic organisms from vertebrate (Žegura & Filipič, 2019;Camargo Filho et al, 2022;Cant et al, 2022;Costa, 2022;Gazo et al, 2022;Mahboob et al, 2022) to invertebrate (Erraud et al, 2018;Khan et al, 2019;Guidi et al, 2021;Cosentino et al, 2022;Ivorra et al, 2022) Mussels (Mytilus edulis and their relatives) are a very common aquatic invertebrate species that have been used as sentinel organisms in aquatic environment monitoring since Goldberg (1975) has proposed the Mussel Watch program. Mussels were thought to fulfil what was required by environmental monitoring principles because of their behaviour as a sessile organism, wide geographical distribution, availability, and resistance to general stress.…”

Section: Introductionmentioning

confidence: 99%

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

Yaqin

2022

Akuatikisle: J. Aqua. Coast. & Isle

View full text Add to dashboard Cite

Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour. The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters. DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro. The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site. Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.

show abstract

How can environmental conditions influence dicofol genotoxicity on the edible Asiatic clam, Meretrix meretrix?

Cited by 3 publications

References 38 publications

To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse

To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse

Spontaneous In-Source Fragmentation Reaction Mechanism and Highly Sensitive Analysis of Dicofol by Electrospray Ionization Mass Spectrometry

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

Contact Info

Product

Resources

About