The formation of a network of protected objects under the conditions of the urbanized landscape is one of the effective measures for implementing the concept of sustainable urban development. The transformation of natural ecosystems within settlements causes significant changes in the structure of zoocenoses: some species disappear from the territory; others are forced to adapt to new conditions. Reducing the level of anthropogenic pressure in the created protected objects due to the limitation of certain types of economic activity will allow preserving the species wealth of fauna even under the conditions of the urban landscape. Work was carried out on the study of the taxonomic wealth of the vertebrate fauna of one of the tracts of the city of Vinnytsia for the further preparation of the scientific justification for the creation of a protected object within its boundaries. To study the seasonal structure of zoocenoses of chordate animals, the tract 'Brigantyna' was chosen, which is located on the left bank of the Sabariv reservoir (Southern Bug river), the mouth of the Tyazhilivka river and the surrounding area. The total area of the studied territory is about 5.1 hectares. Generally accepted methods were used to study the species composition of the chordate animals of the tract. During all seasonal periods of 2020-2022, the seasonal structure and nature of topical connections of representatives of 5 classes of Chordata were studied within the facility: class Actinopterygii, Amphibia, Reptilia, Aves, Mammalia. For the first time, the taxonomic and ecological structure of the zoocenoses of the tract were analyzed, in its species structure representatives were identified, which are subject to protection at the local or global level. Within the studied territory, as a result of the records, the presence of representatives of 99 species of animals of the Chordata type was found: class Actinopterygii (16 species), Amphibia (4 species), Reptilia (4 species), Aves (66 species), Mammalia (9 species). Taxonomically, they are united in 79 genera, 42 families and 21 orders. The vast majority of discovered chordates are typical representatives of the fauna of Eastern Podillia. The creation of a protected object within the surveyed tract will contribute to the preservation of the biological diversity of the urban zoocenosis.