How do neutrophils and pathogens interact?

Mayer‐Scholl, Anne; Averhoff, Petra; Zychlinsky, Arturo

doi:10.1016/j.mib.2003.12.004

Cited by 119 publications

(81 citation statements)

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“…Ainda, esta lectina pode desfavorecer a produção de EROs em neutrófilos não ativados por regular a localização de elementos de sinalização celular em microdomínios lipídicos da superfíce celular "Lipid Rafts" (PRIOR et al, 2003) e estes microdomínios são importantes para a produção adequada de EROS por estes leucócitos (FUHLER et al, 2007). Com base nestes achados, sugere-se que a Gal-1 pode participar do ajuste da produção de EROs, desfavorecendo, por um lado, lesões teciduais mediadas pela produção de radicais livres na ausência de infecção (SMITH, 1994) e favorecendo, por outro, a eliminação de agentes infecciosos no local da inflamação por otimizar a produção de EROs de neutrófilos ativados (MAYER-SCHOLL et al, 2004).…”

Section: Discussionunclassified

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

Rodrigues¹

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Section: Discussionunclassified

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

Rodrigues¹

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“…Além do núcleo multi-lobulado (com 3 a 5 lóbulos), os neutrófilos apresentam formato esférico com cerca de 12 a 15 µm de diâmetro, e grânulos em abundância no citoplasma. Tais grânulos são classificados como azurófilos (ou primários), específicos (ou secundários), de gelatinase (ou terciários) e vesículas secretoras (Mayer-Scholl et al, 2004;Borregaard et al, 2007).…”

Section: Aspectos Geraisunclassified

“…A destruição dos patógenos ocorre tanto através de mecanismos independentes de oxigênio (com a ação das substâncias tóxicas contidas nos grânulos) como através de mecanismos dependentes de oxigênio (com a produção de ERO com grande potencial microbicida) (Lee et al, 2003;Mayer-Scholl et al, 2004;Amulic et al, 2012;Kolaczkowska;Kubes, 2013).…”

Section: -Fagocitoseunclassified

“…A fusão dos grânulos com a membrana plasmática ou com a membrana dos fagossomas e a liberação das substâncias microbicidas podem causar a morte dos patógenos. Tais substâncias microbicidas são, principalmente, proteínas e enzimas antimicrobianas como a defensina e a lisozima (que funcionam rompendo a superfície aniônica bacteriana) e proteases (que degradam proteínas bacterianas) (Mayer-Scholl et al, 2004;Pham, 2006;Bardoel et al, 2014). (Faurschou;Borregaard, 2003;Mayer-Scholl et al, 2004;Klüter et al, 2014).…”

Section: -O Processo De Desgranulaçãounclassified

“…A NADPH oxidase reduz o oxigênio molecular presente no meio a ânion superóxido (O 2 •-), que é a primeira ERO formada e a precursora de todas as demais produzidas durante o metabolismo oxidativo (Freitas et al, 2009;Bardoel et al, 2014 (Mayer-Scholl et al, 2004;Freitas et al, 2009;van der Veen et al, 2009;Klebanoff et al, 2013 (Freitas et al, 2009;Amulic et al, 2012;Klebanoff et al, 2013;.…”

Section: -Produção De Espécies Reativas De Oxigênio (Ero)unclassified

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O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Rinhel¹

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RibeirãoNeutrophils are phagocytic immune cells mobilized primarily to fight against infections. These cells are capable to kill microorganisms through degranulation, which leads to the release of antimicrobial molecules, and production of reactive oxygen species. Although neutrophil recruitment is essential for host protection, excessive activation of these cells culminates in intense discharge of cytotoxic molecules to the extracellular milieu, which can elicit the development of and/or aggravate the clinical condition in autoimmune and inflammatory diseases such as rheumatoid arthritis. Modulation of the effector functions of neutrophils is a possible therapeutic approach to treat these diseases. Previous studies have shown that the ethanolic extract of Baccharis dracunculifolia leaves (EEBd) has a great therapeutic potential due to its significant antioxidant effect towards the oxidative metabolism of neutrophils. To continue investigating the anti-inflammatory and/or immunomodulatory action of such extract, the first part of the present work examined its modulator effect on three important effector functions of human neutrophils that mediate recognition and clearance of pathogens -phagocytosis, degranulation, and microbial killing -and on the activity of lysosomal enzymes. EEBd inhibited the oxidative metabolism of neutrophils without affecting their defense ability, depending on the extract concentration used. To propose possible chemical markers for the biological effects of the extract, the second part of this study evaluated the antioxidant activity of seven compounds isolated from EEBd -caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether, isosakuranetin, and hispidulin -towards the oxidative metabolism of neutrophils. The results pointed to caffeic acid as a potential chemical marker for this biological activity. The third part of this investigation aimed to improve the bioavailability and favor the therapeutic use of the natural products studied. EEBd and caffeic acid were incorporated into liposomal carrier systems. The incorporation of both samples into liposomes did not alter their antioxidant activity towards neutrophils, as compared with their respective free forms. Finally, this study examined the therapeutic effect of EEBd and caffeic acid, both in the free form and incorporated into liposomes, in an animal model of arthritis. The free samples markedly improved the joint inflammatory parameters: they reduced edema and migration of total cells and neutrophils, and slightly lowered the levels of the inflammatory cytokines tumor necrosis factor  and interleukin 6 and 1 (TNF-, IL-6, and IL-1, respectively). In addition, incorporation of EEBd and caffeic acid into liposomes can help to reduce their concentrations required to achieve the desired therapeutic effect. Based on the set of results obtained in this study, we conclude that EEBd has a great potential to become a therapeutic adjuvant in the treatment of neutrophil-mediated inflammatory diseases, and that...

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Leveraging H₂O₂ Levels for Biomedical Applications

et al. 2017

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Hydrogen peroxide (H 2 O 2 )-responsive materials have been employed as drug delivery or diagnosis systems to treat or detect diseases with abnormal oxidative stress. A number of H 2 O 2 -responsive systems have been developed and they have achieved great progress in controlled drug delivery for disease treatment. However, pathological sites with elevated H 2 O 2 level, such as cancer and inflammation, have their own characteristics, therefore the design of material structures and the subsequent formulations should be reasonably designed to acquire maximized therapeutic effects.In this progress report, we overview the development of H 2 O 2 -responsive functional groups for constructing H 2 O 2 -responsive formulations, as well as the guidance for designing suitable formulations to treat each specific pathological condition. The challenges and perspectives in this field are also discussed.Hydrogen peroxide (H 2 O 2 )-stimulated systems have recently been employed to treat pathologic sites with abnormal oxidative stress. Here, an overview over the designing principles and strategies of drug delivery systems composing of H 2 O 2 -responsive materials for cancer, inflammation and diabetes is provided. Additionally, the diagnosis of diseases using H 2 O 2 -responsive fluorescent compounds is discussed.

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How do neutrophils and pathogens interact?

Cited by 119 publications

References 30 publications

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Leveraging H₂O₂ Levels for Biomedical Applications

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How do neutrophils and pathogens interact?

Cited by 119 publications

References 30 publications

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

Avaliação do impacto biológico da Galectina-1, endógena e exógena, sobre funções de neutrófilos

O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Leveraging H2O2 Levels for Biomedical Applications

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Leveraging H₂O₂ Levels for Biomedical Applications