How Does Fat Survive and Remodel After Grafting?

Mashiko, Takafumi; Yoshimura, Kotaro

doi:10.1016/j.cps.2014.12.008

Cited by 136 publications

(105 citation statements)

References 18 publications

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“…In addition, administration of small volumes (less than 0.1 ml per pass) of fat and multiple passes ensures improved fat graft take, since implanted tissue is easily vascularized and not exposed to prolonged hypoxia. 10,29,30 Cell-assisted lipotransfer, a procedure in which stromal vascular fraction cells are isolated and then mixed with adipose-tissue before re-injection, has been suggested to improve graft take. 31 However, the process to isolate ASCs from adipose tissue by collagenase digestion cannot be considered as "minimal manipulation" and requires compliance with good manufacturing process technical standards and regulatory limitations.…”

Section: Discussionmentioning

confidence: 99%

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Trivisonno¹,

Rossi²,

Monti³

et al. 2017

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Please cite this article as: Trivisonno, A.; Rossi, A.; Monti, M.; Di Nunno, D.; Desouches, C.; Cannistra, C.; Toietta, G. Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis. Journal of Plastic Reconstructive and Aesthetic Surgery 2017, 70, 1118-1128. DOI: 10.1016/j.bjps.2017 http://www.sciencedirect.com/science/article/pii/S1748681517301559 M A N U S C R I P T A C C E P T E D M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 2 SUMMARYCumulative, long-term exposure to solar ultraviolet radiation promotes premature skin aging characterized by wrinkle formation and reduced skin elasticity. In this study, we assessed whether microfat transfer could improve dermal and subcutaneous tissue thickness loss associated with photoaging. Twenty one patients affected by facial photoaging (photodamage grade II-IV; age range 35-62 years; 19 females, 2 males; all of Caucasian origin) were treated using minimally-invasive autologous dermal white fat transfer harvested with a recently designed microcannula. The results were determined by clinical assessment, patient self-evaluation and quantified by the Antera 3D ® dermal digital device for non-invasive, objective, reliable and accurate assessment of facial skin texture, color and wrinkle characteristics. Compared with the pretreatment condition, the increment in soft tissue volume and improvement in skin quality and texture was assessed by a dermatologist after treatment. In addition, instrumental evaluation by digital skin profilometry of the treated areas revealed:a 41% reduction in average wrinkle depth (7.29 ± 1.04 x 10 -2 mm pre-treatment vs. 4.31 ± 1.16 x 10 -2 mm at 90 days post-treatment; p < 0.001), improved skin texture, more homogeneous and uniform skin color and declined facial hemoglobin and melanin concentrations. The majority of patients (above 90%) reported improvements in self-perception. No significant complications were reported throughout the study. In conclusion, by using digital profilometry analysis as an objective and innovative tool to determine the outcome of treatment, we demonstrated that autologous microfat transfer is a safe and well-tolerated procedure with measurable beneficial effects on facial skin aging.

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Section: Discussionmentioning

confidence: 99%

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Trivisonno¹,

Rossi²,

Monti³

et al. 2017

Journal of Plastic, Reconstructive & Aesthetic Surgery

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“…Despite a variety of commercial products including synthetic implants, injectable filling material, and homologous or human-derived autografts, autologous fat transplantation is more commonly performed, due to its good tissue compatibility with minimal rejection reactions and donor site defects [1,2]. Since Neuber [1] first reported autologous fat transplantation, several technical and basic science landmark progress of autologous fat transplantation has been made including tumescent liposuction [1], structure lipotransfer [2], three zone theory [3,4], cell assisted lipotransfer (CAL) [5,6] and SVF-gel [7]. Nevertheless, the varying retention rate [8,9] after transplantation remains to be solved and when applied to large defects like breast reconstruction, a high number of grafting procedures are required [10].…”

Section: Introductionmentioning

confidence: 99%

Introduction of ligated vessels promote the retention and regeneration of free fat: constructing a fat flap in tissue engineering chamber

et al. 2020

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Background: Breast reconstruction with fat grafting has an unstable retention rate due to insufficient revascularization. Tissue Engineering Chamber (TEC) model can promote tissue regeneration in the chamber by introducing ligated vessels around the tissue. We introduced ligated vessels with free fat graft to investigate the retention rate and revascularization of grafted fat that in TEC model. Methods: SD rats (n=24) was divided into 3 groups randomly. Group A: Standard TEC model was constructed; Group B: the epigastric vessel bundles were dissected from the fat flap and ligated, fat flap was cut into granules and planted into the chamber; Group C: Free fat was planted in the chamber. At week 6, samples in the chamber were harvested. Results: Significant volume increase was observed in group A and B, while the volume decreased in group C (P<0.05). Regeneration morphology could be found according to the histological observation in A and B. Micro CT results showed the ligated vessels into grafted fat sprouting robustly, coordinated with volume changes. Conclusion: Fat grafts in TEC model could not only survive but also regenerate. The combination of fat graft and TEC could fabricate a vascularized fat flap, which was a promising method in breast reconstruction.

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“…Its main upside is the extremely harmless for mature adipocytes, leaving them intact and, therefore, less oil is released from their rupture . Another advantage of decantation is the great retention of SVF cells; indeed, gravity alone preserves such cells that are localized in the pericapillary area and intimately close to mature adipocytes . By contrast, centrifugation tends to detach SVF cells including them into the pellet .…”

mentioning

confidence: 99%

“…11,17 Another advantage of decantation is the great retention of SVF cells; indeed, gravity alone preserves such cells that are localized in the pericapillary area and intimately close to mature adipocytes. 18 By contrast, centrifugation tends to detach SVF cells including them into the pellet. 11,16 The main disadvantages of the decantation-based procedures are both lacking in concentration of beneficial cells and inadequate purification of harvested tissues from blood cells/contaminants, since the stratification process can only be partial within times compatible with a surgical operation.…”

mentioning

confidence: 99%

Lull pgm system: A suitable technique to improve the regenerative potential of autologous fat grafting

Morselli¹,

Giorgini²,

Pazzini

et al. 2017

Wound Repair Regeneration

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Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, that is, the decantation and Coleman's centrifugation techniques. The lowest number of erythrocytes, which are proinflammatory cells, was observed in the Lull samples, corresponding to about 50% of those isolated by decantation and centrifugation. The highest amount of SVF cells were isolated from the Lull samples whose number of colony forming units, representative of the amount of adipose-derived stem cells (ADSCs), was about fourfold and sixfold higher than in the decantation and centrifugation samples, respectively. Adipocyte and osteoblast commitment of SVF cells obtained from all the three procedures also confirmed that the subpopulation of ADSCs was actively represented in the processed aspirates. Moreover, the growth rate of the SVF cells was more accentuated in the samples obtained from decantation and Lull than centrifugation. In conclusion, Lull seems to be the best processing technique for adipose tissue graft with respect to decantation and centrifugation, because it clears more efficiently the fat from proinflammatory blood cells and provides the greatest number of proliferating SFV cells and ADSCs.

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How Does Fat Survive and Remodel After Grafting?

Cited by 136 publications

References 18 publications

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Introduction of ligated vessels promote the retention and regeneration of free fat: constructing a fat flap in tissue engineering chamber

Lull pgm system: A suitable technique to improve the regenerative potential of autologous fat grafting

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