How hyaluronan–protein complexes modulate the hyaluronidase activity: The model

Abstract

Please cite this article as: Jean-Claude Vincent, Hélène Lenormand, How hyaluronanprotein complexes modulate the hyaluronidase activity: The model, Biophysical Chemistry (2009Chemistry ( ), doi: 10.1016Chemistry ( /j.bpc.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Simulations performed by introducing experimental data produce a theoretical behaviour similar to the experimental one, including all the atypical phenomena observed : substratedependence, enzyme-dependence and protein-dependence of HAase. This shows that our assumptions are sufficient to explain the behaviour of the system and allows us to estimate unknown parameters and suggest new developments. A C C E P T E D

pH effects on the hyaluronan hydrolysis catalysed by hyaluronidase in the presence of proteins: Part I. Dual aspect of the pH-dependence

“…HAase activity thus depends on the concentration of its substrate in a very unusual manner (Astériou et al, 2006). The mechanism responsible for this has been clearly shown by modelling (Lenormand et al, 2007;Vincent and Lenormand, 2009). In the following text, unless otherwise mentioned, by "complexes" we mean "non-specific electrostatic complexes" and by "HAase" we mean "bovine testicular HAase".…”

Hyaluronidase Inhibitory Activity of Pentacylic Triterpenoids from Prismatomeris tetrandra (Roxb.) K. Schum: Isolation, Synthesis and QSAR Study

“…It can be found in an extracellular matrix, especially in soft connective tissues of all vertebrates and in the capsule of some bacteria [ 2 ]. HA plays an important role in biological processes such as cellular adhesion, mobility differentiation processes that act as lubricant and shock absorber, regulates water balance and osmotic pressure [ 3 ]. It is also the structure-forming molecule in the vitreous humor of the eye, in Wharton’s jelly and in joint fluids.…”

“…Hyaluronidase degrades HA by cleaving the N -acetylglucosamidic bonds of HA via a β-elimination process to produce HA oligosaccharides, with chain lengths of four to 25 disaccharides which possess angiogenic properties [ 3 , 4 ]. The process of HA oligosaccharide formation favors the production of new blood vessels, thus facilitating the development of cancer tumors.…”

Biological properties of butanol extracts from green pine cone of Pinus densiflora

“…HAase is involved in inflammation, cancer metastasis, and permeability of the vascular system. Therefore, modulation of HAase inhibition will be useful for maintaining the normal homeostasis in the body (Girish and Kemparaju, 2007;Vincent and Lenormand, 2009). Accordingly, an evaluation of HAase inhibition could be considered a useful method for identifying the anti-inflammatory activity during the screening of biological active compounds.…”