How membrane lipids influence plasma delivery of reactive oxygen species into cells and subsequent DNA damage: an experimental and computational study

Paal, Jonas Van der; Hong, Sung-Ha; Yusupov, Maksudbek; Gaur, Nishtha; Oh, Jun‐Seok; Short, Robert D.; Szili, Endre J.; Bogaerts, Annemie

doi:10.1039/c9cp03520f

Cited by 32 publications

(24 citation statements)

References 73 publications

(77 reference statements)

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“…Some of these factors include cancer cells generally having a higher number of aquaporin channels and altered levels of certain lipid types and cholesterol in such a way that these features reduce the barrier effects of the cell membrane to ROS [8][9][10][11][12]. These features allow cancer cells to easily uptake plasma generated ROS in certain circumstances, which makes these cells more susceptible to oxidative damage by the plasma treatment [13][14][15]. Plasma induced oxidative stress may also disrupt biomolecular binding events to inhibit proliferative signalling pathways in cancer cells [16].…”

Section: Introductionmentioning

confidence: 99%

Low-temperature Plasma Jet Activated Media Induces Apoptosis in Bladder Cancer Cells via the Cytochrome c/caspase 3 Pathway

Fukuhara

Szili²,

et al. 2021

Preprint

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Current methods used to treat non-muscle invasive bladder cancer are inadequate due to a high recurrence rate after surgery and occurrence of adverse events such as interstitial pneumonia following intravesical instillation therapy. Low-temperature plasma is a new form of physical therapy that provides a rich source of reactive oxygen species (ROS). Oxidative solutions, created by pre-treatment of aqueous media with plasma before application to target cells, lead to the destruction of cancer cells through oxidative stress pathways. This study focuses on the effects of plasma activated media (PAM) in bladder cancer cells. PAM treatment increases up regulation of p21 and down regulation of Cyclin D and CdK4 leading to cell cycle arrest, and concomitantly depolarises the mitochondrial membrane leading to increased mitochondrial ROS production. Cell cycle arrest and increased mitochondrial ROS production induce apoptosis in bladder cancer cells in vitro and in a bladder cancer tumour in vivo via the pro-apoptotic caspase 3/cytochrome c pathway. These observations highlight the potential of plasma activated solutions as a new adjuvant therapy in the clinical treatment of bladder cancer.

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Section: Introductionmentioning

confidence: 99%

Low-temperature Plasma Jet Activated Media Induces Apoptosis in Bladder Cancer Cells via the Cytochrome c/caspase 3 Pathway

Fukuhara

Szili²,

et al. 2021

Preprint

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“…As reported by van der Paal et al, [37] the ROS generated by the plasma could pass through cell membranes, which contain unsaturated lipids or cholesterol. The dead keratinocytes and lipid matrix of SC contain abundant unsaturated lipids and cholesterol.…”

Section: The Effect Of Ros On Tdd Efficiencymentioning

confidence: 76%

Cold atmospheric plasma jet array for transdermal drug delivery

Nie

Duan

et al. 2020

Plasma Processes & Polymers

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Transdermal drug delivery (TDD) is an important drug delivery method, and cold atmospheric plasma has been proven its potential as a TDD approach. In this study, a 9-channel plasma jet array was designed to enhance TDD efficiency. The Franz diffusion experiment showed that the TDD efficiency of the drug across the skin was enhanced about 2-110 times after the plasma jet array treatment. The histological analysis showed that the structure of the stratum corneum could be destroyed by the plasma. Further investigations indicated that the reactive oxygen species of the plasma jet play a key role in enhancing TDD efficiency. Our results indicated that the plasma jet array is a potential and effective TDD approach.

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“…For plasma medicine, molecules involved in this process might be related to, for instance, the Nrf2 pathway [ 36 , 74 , 75 ], heme oxygenase 1 (HMOX1) [ 76 ], or other antioxidant response elements [ 77 ]. Others have suggested that the lipid composition of tumor cells [ 78 , 79 , 80 ] and the expression of aquaporins [ 81 , 82 , 83 ] are also important for the selectivity of plasmas towards malignant cells. Plasma generates different ROS, such as ozone (O 3 ), superoxide (O 2 − ), singlet delta oxygen ( 1 O 2 ), atomic oxygen (O), hydroxyl radical ( • OH), and hydrogen peroxide (H 2 O 2 ) [ 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ].…”

Section: Discussionmentioning

confidence: 99%

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Pasqual-Melo

Nascimento

Sanches

et al. 2020

Cancers

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Cutaneous squamous cell carcinoma (SCC) is the most prevalent cancer worldwide, increasing the cost of healthcare services and with a high rate of morbidity. Its etiology is linked to chronic ultraviolet (UV) exposure that leads to malignant transformation of keratinocytes. Invasive growth and metastasis are severe consequences of this process. Therapy-resistant and highly aggressive SCC is frequently fatal, exemplifying the need for novel treatment strategies. Cold physical plasma is a partially ionized gas, expelling therapeutic doses of reactive oxygen and nitrogen species that were investigated for their anticancer capacity against SCC in vitro and SCC-like lesions in vivo. Using the kINPen argon plasma jet, a selective growth-reducing action of plasma treatment was identified in two SCC cell lines in 2D and 3D cultures. In vivo, plasma treatment limited the progression of UVB-induced SSC-like skin lesions and dermal degeneration without compromising lesional or non-lesional skin. In lesional tissue, this was associated with a decrease in cell proliferation and the antioxidant transcription factor Nrf2 following plasma treatment, while catalase expression was increased. Analysis of skin adjacent to the lesions and determination of global antioxidant parameters confirmed the local but not systemic action of the plasma anticancer therapy in vivo.

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How membrane lipids influence plasma delivery of reactive oxygen species into cells and subsequent DNA damage: an experimental and computational study

Abstract: The combination of phospholipid vesicle experiments and molecular dynamics (MD) simulations illustrate how lipid oxidation, lipid packing and rafts formation may influence the response of healthy and diseased cell membranes to plasma-derived RONS.

Cited by 32 publications

References 73 publications

Low-temperature Plasma Jet Activated Media Induces Apoptosis in Bladder Cancer Cells via the Cytochrome c/caspase 3 Pathway

Low-temperature Plasma Jet Activated Media Induces Apoptosis in Bladder Cancer Cells via the Cytochrome c/caspase 3 Pathway

Cold atmospheric plasma jet array for transdermal drug delivery

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

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