How Similar Are Inbred Rats? The Influence of Anatomical Variations, Shipment and Sampling Time on Experimental Surgery

Jin, Hao; Huang, Hai; Zhang, J.; Dirsch, Olaf; Dahmen, Uta

doi:10.1159/000333395

Cited by 1 publication

(1 citation statement)

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“…This becomes apparent by a positive correlation between organ and organism mass [32]. In order to test whether portal blood supply is crucial for liver lobe size regulation, portal vein ligation (PVL) experiments are performed.…”

Section: Methodsmentioning

confidence: 99%

How Does a Single Cell Know When the Liver Has Reached Its Correct Size?

et al. 2014

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The liver is a multi-functional organ that regulates major physiological processes and that possesses a remarkable regeneration capacity. After loss of functional liver mass the liver grows back to its original, individual size through hepatocyte proliferation and apoptosis. How does a single hepatocyte ‘know’ when the organ has grown to its final size? This work considers the initial growth phase of liver regeneration after partial hepatectomy in which the mass is restored. There are strong and valid arguments that the trigger of proliferation after partial hepatectomy is mediated through the portal blood flow. It remains unclear, if either or both the concentration of metabolites in the blood or the shear stress are crucial to hepatocyte proliferation and liver size control. A cell-based mathematical model is developed that helps discriminate the effects of these two potential triggers. Analysis of the mathematical model shows that a metabolic load and a hemodynamical hypothesis imply different feedback mechanisms at the cellular scale. The predictions of the developed mathematical model are compared to experimental data in rats. The assumption that hepatocytes are able to buffer the metabolic load leads to a robustness against short-term fluctuations of the trigger which can not be achieved with a purely hemodynamical trigger.

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Section: Methodsmentioning

confidence: 99%