How stereochemistry affects the physicochemical features of gemini surfactant based cationic liposomes

Abstract

The physicochemical features of mixed cationic liposomes formulated with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcoline and one of three stereomeric cationic gemini surfactants were investigated by differential scanning calorimetry, fluorescence, TEM and dynamic laser light scattering. Actually, it was shown that the different stereochemistry of these gemini can strongly influence their capability of condensing and transfecting a plasmid DNA and of delivering a photosensitizer. The results obtained in this investigation show that the stereochemistry of liposome components and their percentage in the formulation may have dramatic effects on the organization of the lipid bilayer and hence on the morphological and physicochemical features of vesicles, thus being highly relevant to the biological features evaluated previously

Rifampicin–Liposomes for Mycobacterium abscessus Infection Treatment: Intracellular Uptake and Antibacterial Activity Evaluation

“…Samples for TEM were prepared on copper grids covered with a carbon film. The vesicle dispersions were pipetted onto the grids and stained with 1% phosphotungstic acid (pH adjusted to 7.4) as performed in previous investigations [ 22 ]. Measurements were carried out by means of a FEI TECNAI 12 G2 Twin (FEI Company, Hillsboro, OR, USA), operating at 120 kV and equipped with an electron energy filter (Gatan image filter) and a slow-scan charge-coupled device camera (Gatan multiscan).…”

Effect of surfactant structure on the mixed micelle formation of cationic gemini–zwitterionic phospholipid systems

“…Even stereochemistry of spacer affects their aggregation properties. Aleandri et al [25] studied mixed cationic liposomes formulated with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcoline and three stereomeric forms of cationic gemini surfactant 2,3-dimethoxy-1,4-bis(N-hexadecyl-N,Ndimethylammonium) butane dibromide, by differential scanning calorimetry, fluorescence, TEM and dynamic laser light scattering. They found that different stereochemistry of these gemini strongly affect their capability of condensing and transfecting a plasmid DNA and of delivering a photosensitizer.…”

“…A is the surface area of the head group. v and l c can be easily obtained using Tanford's formulas [52]: v = 27.4 +26.9n (25) l c = 1.54 + 1.265n (26) n is the number of carbon atoms present in the hydrophobic tails. As PL and gemini both have two tails in a molecule, the volume obtained for single tail was then doubled to get v. Therefore, R p value decreases and small micelles are formed.…”

How stereochemistry of lipid components can affect lipid organization and the route of liposome internalization into cells

“…12 In fact, it is known that the uptake of caveolae-mediated endocytosis occurs at a much slower rate than that of clathrin-mediated endocytosis. 6 We know from a previous investigation 17 that DMPC/1a liposomes are characterized by a higher transition temperature and by a minor extent of lipid miscibility with respect to DMPC/1b liposomes. It was also found that DMPC/1a liposomes feature a surface potential, Ψ s , higher than DMPC/1b liposomes.…”