How to use the development of individual Drosophila larvae as a metabolic sensor

Trautenberg, Laura Christin; Knittelfelder, Oskar; Hofmann, Carla; Shevchenko, Andrej; Brankatschk, Marko; Prince, Elodie

doi:10.1016/j.jinsphys.2020.104095

Cited by 3 publications

(6 citation statements)

References 38 publications

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“…Yeast-free food fed animals have limited IPC activity, low systemic IS levels and show decreased survival at high temperatures (1). To rule out the effects of yeast products, we have repeated all experiments using C orn f ood (CF), a yeast-free diet that resembles the ingredient proportions found in YF ((39); Table 1 and Table 2, respectively). At 28°C, controls are unable to survive on CF.…”

Section: Resultsmentioning

confidence: 99%

“…We found that D7N never show high Ca 2+ levels on CF contrary to flies kept on YF (Figure 1). To increase Ca 2+ yields in D7Ns, we expressed the temperature-sensitive Ca 2+ channel, TRPA1, and assayed the survival of larvae kept on yeast-free CF ((39) and Table recipes). Interestingly, survival rates of dIlp 7 >> TRPA1 ( TRPA1 ) larvae increased significantly (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…It was shown that dIlp7 expression is strongly dependent on nutritional variables including protein:sugar ratio and caloric load (47). CF is rich in calories, its protein:sugar ratio is optimized and contains about 10% lipids ((39) and Table 1). Nevertheless, on CF, systemic Insulin levels are low and wild-type larvae struggle to complete their development (39,48).…”

Section: Discussionmentioning

confidence: 99%

“…CF is rich in calories, its protein:sugar ratio is optimized and contains about 10% lipids ((39) and Table 1). Nevertheless, on CF, systemic Insulin levels are low and wild-type larvae struggle to complete their development (39,48). Astonishingly, we found that the absence of both dIlp2 and dIlp7 improved larval survival on CF up to numbers shown by controls.…”

Section: Discussionmentioning

confidence: 99%

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Diet and heat - one neuronal subset two responses

Prince¹,

Kretzschmar²,

Trautenberg³

et al. 2020

Preprint

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The Insulin signal cascade is one of the best studied metabolic circuits, and shows a remarkable high molecular and functional conservation across the animal kingdom. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control is rapid and integrates a wide range of variables including dietary change or environmental temperature. However, despite various detailed studies that demonstrated the potential of neuronal regulation the physiological relevance of this circuit remains elusive.In Drosophila, Insulin-like peptide 7 (dIlp7)-producing neurons are wired with Insulin-producing cells. We found a dual role for this neuronal subset: a.) activated dilp7-producing neurons are required to facilitate development at high temperatures, and if confronted with calorie-rich food that represses neuronal activity b.) their product, dIlp7, regulates Insulin signalling levels. Our work shows that Insulin-producing cells not simply integrate signals from circulating nutritional cues and neuronal inputs, but switch to neuronal control in response to dietary composition.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diet and heat - one neuronal subset two responses

Prince¹,

Kretzschmar²,

Trautenberg³

et al. 2020

Preprint

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“…Yeast food was prepared as published by Brankatschk et al (2018) (Table 1). Corn food recipe is described in Table 2 and in Trautenberg et al (2020).…”

Section: Dietsmentioning

confidence: 99%

DIlp7-Producing Neurons Regulate Insulin-Producing Cells in Drosophila

Prince

Kretzschmar²,

Trautenberg³

et al. 2021

Front. Physiol.

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Cellular Insulin signaling shows a remarkable high molecular and functional conservation. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control integrates a wide range of variables including dietary change or environmental temperature. Although it is shown that neuronal input is sufficient to regulate Insulin-producing cells, the physiological relevance of this network remains elusive. In Drosophila melanogaster, Insulin-like peptide7-producing neurons are wired with Insulin-producing cells. We found that the former cells regulate the latter to facilitate larval development at high temperatures, and to regulate systemic Insulin signaling in adults feeding on calorie-rich food lacking dietary yeast. Our results demonstrate a role for neuronal innervation of Insulin-producing cells important for fruit flies to survive unfavorable environmental conditions.

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Uric acid metabolism modulates diet-dependent responses to intraspecific competition in Drosophila larvae

Morimoto¹

2022

iScience

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How to use the development of individual Drosophila larvae as a metabolic sensor

Cited by 3 publications

References 38 publications

Diet and heat - one neuronal subset two responses

Diet and heat - one neuronal subset two responses

DIlp7-Producing Neurons Regulate Insulin-Producing Cells in Drosophila

Uric acid metabolism modulates diet-dependent responses to intraspecific competition in Drosophila larvae

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